The experiment was conducted at the Laboratory of Biotechnology, Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur- 1701 during July 2007 to June 2008. An efficient and reproducible protocol for the production of transgenic potato plants was developed by inoculating internode explants of potato with Agrobacterium tumefaciens strain LBA4404 carrying a binary vector pBI121 having one reporter gene (gus) and selectable marker gene (nptII) resistant to Kanamycin. The transformation experiment was done by optimizing two important parameters names infection time and cocultivation period. Most of the explants produced shoots within 21 days on 5 mg/l Zeatin riboside (ZR) and 50 mg/l Kanamycin supplemented MS medium without introducing callus. The infected explants produced 8.27 and 6.42 shoots in Asterix and Diamant varieties, respectively within 21 days. Transgenes were confirmed by molecular analysis. DNA from well established rooted plants confirmed nptII positive through PCR analysis. The transformation rates were 28.97 and 24.37% in Asterix and Diamant, respectively. Putative transformed plants of Diamant and Asterix varieties produced roots in ½MS medium supplemented with 50/mg Cefotaxim, 50 mg/l Kanamycin and 0.5 mg/l IBA. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11220 Bangladesh J. Agril. Res. 37(2): 185-194, June 2012
Cotyledon explants of sweetgourd (Cucurbita moschata Duch.) were cultured on MS supplemented with different concentrations of BAP alone and in combination with NAA, TDZ and 2,4-D. High shooting frequency (94.4%) was obtained from BARI Mistikumra-1 when cotyledon segments were cultured on MS with 4.44 μM BAP alone. The highest number (8.9) of shoot development and minimum days (14.7) required for shoot induction were also observed in 4.44 μM BAP treatment for BARI Mistikumra-1. In contrast, 83.3% shooting frequency were observed from BARI Mistikumra-2 producing 11.9 shoots in 8.4 days on 8.88 μM BAP. The regenerated shoots of both varieties were rooted on half strength of MS and MS supplemented with 0.53 μM NAA and 2.46 μM IBA. Rooting was observed in BARI Mistikumra-1 with 92.6% frequency in 12.3 days whereas it took 8.6 days with 93.3% rooting frequency in BARI Mistikumra-2 when shoots were cultured on MS supplemented with 0.53 μM NAA. Welldeveloped rooted plantlets were transferred to pots containing sterile soil and covered with polythene bags in the greenhouse for hardening. The acclimated plants were planted in the field after three weeks.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.