In vitro Regeneration of Sweetgourd (Cucurbita moschata Duch.)

Abstract

Cotyledon explants of sweetgourd (Cucurbita moschata Duch.) were cultured on MS supplemented with different concentrations of BAP alone and in combination with NAA, TDZ and 2,4-D. High shooting frequency (94.4%) was obtained from BARI Mistikumra-1 when cotyledon segments were cultured on MS with 4.44 μM BAP alone. The highest number (8.9) of shoot development and minimum days (14.7) required for shoot induction were also observed in 4.44 μM BAP treatment for BARI Mistikumra-1. In contrast, 83.3% shooting frequency were observed from BARI Mistikumra-2 producing 11.9 shoots in 8.4 days on 8.88 μM BAP. The regenerated shoots of both varieties were rooted on half strength of MS and MS supplemented with 0.53 μM NAA and 2.46 μM IBA. Rooting was observed in BARI Mistikumra-1 with 92.6% frequency in 12.3 days whereas it took 8.6 days with 93.3% rooting frequency in BARI Mistikumra-2 when shoots were cultured on MS supplemented with 0.53 μM NAA. Welldeveloped rooted plantlets were transferred to pots containing sterile soil and covered with polythene bags in the greenhouse for hardening. The acclimated plants were planted in the field after three weeks.

Optimization of an Efficient Direct Shoot Induction System and Histological Evaluation of Shoot Formation in Cucurbita maxima Duch

“…Obtaining effective regenerated plants from commercial genotypes is a prerequisite for applying genetic transformation techniques for crop improvement [32]. Differences in embryo and shoot induction rates have been observed even when cultured on the same medium in plants such as summer squash (C. pepo) [25] and pumpkin (C. moschata) varieties [33]. This experiment evaluated the effect of genotype on regeneration using three widely cultivated varieties (L-1, L-2, and L-3) in China and two inbred lines (L-4 and L-5) from Hunan Province.…”

“…Kurtar et al rooted C. maxima and C. moschata plants on MS media supplemented with 0.01 mg/l IAA [42]. Kabir et al also used this medium to induce root growth in sweet gourd (C. moschata) [33]. We evaluated whether the rooting duration, rooting rate, and average rooting number of the winter squash plants were variable in the five different culture media (Table 6).…”

Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance

“…Shoot regeneration on the relationship between organogenic capacity and endogenous hormonal contents in pumpkin (C. moschata) was reported by Zhang et al (2008). In another study, cotyledon explants of sweetgourd (C. moschata) were cultured on MS culture medium supplemented with different plant growth regulators reaching a high shooting frequency (Kabir et al, 2016). Also, the efficiency of haploidization in C. maxima and C. moschata through another culture was evaluated, reaching up to 47.3% of haploid plants (n=20) (Kurtar et al, 2016).…”