Genotoxicity of Thermopsis turcica on Allium cepa L. roots revealed by alkaline comet and random amplified polymorphic DNA assays

Abstract

This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC 50 and 29EC 50 values were 8 and 16 mg/ml concentrations of T. turcica aqueous extracts, respectively. The negative control (distilled water), positive control (methyl methane sulfonate, 10 mg/l) and 8 and 16 mg/ml concentrations of T. turcica extracts were introduced to the roots of onion bulbs for 24 and 96 h. The root growth, DNA damage in root cells and randomly amplified polymorphic DNA (RAPD) profiles of root tissue were used as endpoints of the genotoxicity. The comet assay clearly indicated that dose-dependent single strand DNA breaks in the root nuclei of onions were determined for the treatment concentrations of T. turcica extracts. In comparison to RAPD profile of negative control group, RAPD polymorphisms became evident as disappearance and/or appearance of RAPD bands in treated roots. The diagnostic and phenetic numerical analyses of RAPD profiles obviously indicated dosedependent genotoxicity induced by Thermopsis extracts. In conclusion, the results clearly indicated that water extract of T. turcica has genotoxic potential on the roots of onion bulbs as shown by comet assay and RAPD technique.

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

“…These results indicated that Sargassum dentifolium is more genetically affected by stress condition than D. dichotoma and can be used as a bioindicator. Changes in bands may be the consequences of genomic template instability (GTS) related to the level of DNA damage, the efficiency of DNA repair and replication (Atienzar et al, 2002;Atienzar andJha, 2004 andCiğerci et al, 2016). Yu et al (2013) reported that high genetic variability and differentiation existed among Sargassum horneri populations using ISSR and RAPD.…”

Effects of Fe3O4 Nanoparticle Stress on the Growth and Development of Rocket Eruca sativa

“…Therefore, it is essential to estimate the impact of iron NPs on plant genotoxicity. The randomly amplified polymorphic DNA (RAPD) technique has been efficiently used to evaluate mutagenic effects of NPs on various plant species [6][7][8].…”

iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

“…these results are consistent with earlier studies. Ciğerci et al (2016) showed that GTS % values was significantly decreased in onion bulbs treated with Thermopsis turcica extract for 24h. Changes in bands may be the consequences of genomic template instability, Egypt.…”

“…59, No. 1 (2019) GeNotoxic effects of some plaNt oils aNd abamectiN iN ViGNa uNGuiculata l. ... unguiculata using primers (1-10): 2224, 2229, 2270, 2391, 2392, 2274, 2394, 2370, 2415 and related to the level of DNA damage, the efficiency of DNa repair and replication (atienzar et al, 2002;Atienzar &Jha 2004 andCiğerci et al, 2016).…”