iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

Abstract

E sseNtial oils and the bio-insecticides have been utilized to protect the plants aginst different kind of insects. However, it is really improtent to study the side effects of these essential oils and insecticides on plants. the iPBs technique based on long terminal Repeat (ltR) retrotransposons was applied in this study to detect the DNa damage in cowpea seeds exposed to three plant oils of Trigonella foenum-graecum, Panax ginseng, Origanum majorana and the bio-insecticide abamectin. Different levels of polymorphism ranged from 37.50% polymorphism for primer 2394 to 82.35% polymorphism for primer 2270 with a total average of 52.00%. also, decrease in genetic template stability (Gts%) values were recorded with all treatments as compared with the control. the value of Band sharing index (Bsi) for each treatment was calculated and the results showed that mixture 2 (lC20 abamectin + 2lC95 ginseng) showed the lowest (Bsi) as compared with all treatments. the obtained results, suggested that the assurance should be strongly taken in the consideration for the safety, efficacy, and toxicity of the preparation derived from plants. Furthermore, iPBs assay is a powerful tool for the detection of the genotoxic effects of environmental chemicals and offer great promise for future especially for the determination of genetic damage following exposure to contaminants.