Genotoxicity evaluation of kaurenoic acid, a bioactive diterpenoid present in Copaiba oil

Cavalcanti, Bruno C.; Costa-Lotufo, Letı́cia V.; Moraes, Milton O.; Burbano, Rommel Rodrı́guez; Silveira, Edilberto R.; Cunha, Karina M.A.; Rao, V. S. N.; Moura, Dinara Jaqueline; Rosa, Renato Moreira; Henriques, João Antônio Pêgas; Pessoa, Cláudia

doi:10.1016/j.fct.2005.08.011

Cited by 102 publications

(74 citation statements)

References 31 publications

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“…Moreover, it has been reported that the copaiba oil (Copaifera langsdorffii) had the capacity to reduce the time needed for tissue repair to take place in skin wounds in rats 18,19 , the formation of granulation tissue 20 , while it also showed anti-inflammatory and analgesic properties [19][20][21][22] . Based on positive results with copaiba oil in various models of healing, and taking into account the need for therapeutic resources to improve the viability of skin flaps, this study sought to evaluate the macroscopic and morphometric aspects of random skin flaps treated with 10% copaiba oil (Copaifera langsdorffii) ointment in rats.…”

Section: Introductionmentioning

confidence: 99%

Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats

et al. 2013

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PURPOSE:To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS:Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC -control; GCA -absolute control and GT -treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5μm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION:The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats.

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Section: Introductionmentioning

confidence: 99%

Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats

et al. 2013

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“…Revisão bibliográfica realizada pelos autores evidencia três estudos em que foi possível observar os efeitos citotóxicos da substância. Em um destes, realizado por Cavalcanti et al (2006), ficou evidenciado ainda o efeito genotóxico do ácido caurenoico em altas doses, ou seja, sua capacidade de induzir danos ao DNA.…”

Section: Resultsunclassified

Efeito modulador do óleo de copaíba (Copaifera officinalis L.) sobre a carcinogenicidade da doxorrubicina, avaliado por meio do teste para detecção de clones de tumores em Drosophila melanogaster

Maciel

Magalhães

Orsolin

2017

cmbio

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Introdução: o óleo de copaíba é o composto extraído do tronco da copaibeira (Copaifera sp.) que tem sido utilizado na medicina popular desde a chegada dos portugueses ao Brasil. Objetivo: o objetivo deste estudo foi avaliar possíveis efeitos carcinogênicos e/ ou anticarcinogênicos do óleo de copaíba (Copaifera officinalis L.), por meio do teste para detecção de clones de tumores epiteliais (warts) em Drosophila melanogaster. Metodologia: foram preparadas três soluções de óleo de copaíba, nas proporções de 0,5%, 1% e 2%. Nessas soluções foram cultivadas Drosophilas melanogaster expostas simultaneamente à doxorrubicina na concentração de 0,4 mM, agente conhecidamente cancerígeno, também utilizado como controle positivo na presente pesquisa. Para controle negativo foi utilizado Tween 80 (1%). O tratamento foi realizado com todas as larvas descendentes do cruzamento de fêmeas wts/ TM3 com machos mwh/mwh. Resultados: o óleo de copaíba apresentou atividade carcinogênica quando utilizado isoladamente na concentração 2%, visto que houve aumento estatisticamente significativo (p ≤ 0,05) na frequência de tumores em comparação com o controle negativo. Além disso, evidenciou-se potencialização do efeito carcinogênico da doxorrubicina nas concentrações 0,5% e 1%, uma vez que houve um aumento estatisticamente significativo (p ≤ 0,05) na frequência de tumores nessas concentrações, quando associadas à DXR, em comparação com o controle positivo. Conclusão: os resultados evidenciaram o efeito carcinogênico isolado do óleo de copaíba, bem como seu efeito potencializador quando associado à doxorrubicina

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“…However, lower concentrations (2.5, 5, and 10 μg/mL) failed to induce significant effects, whereas higher concentrations (30 and 60 μg/mL) lead to an increase in cell damage index and frequency. These data indicated that kaurenoic acid induces dose-dependent genotoxicity (Cavalcanti et al, 2006). Dihydrocoumarin is one of the most studied guaco metabolites in regards to its toxic effects.…”

Section: Toxicological Studiesmentioning

confidence: 99%

Mikania glomerata and M. laevigata: Clinical and Toxicological Advances

Gasparetto¹,

Pontarolo²,

Francisco³

et al. 2012

Toxicity and Drug Testing

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Genotoxicity evaluation of kaurenoic acid, a bioactive diterpenoid present in Copaiba oil

Cited by 102 publications

References 31 publications

Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats

Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats

Efeito modulador do óleo de copaíba (Copaifera officinalis L.) sobre a carcinogenicidade da doxorrubicina, avaliado por meio do teste para detecção de clones de tumores em Drosophila melanogaster

Mikania glomerata and M. laevigata: Clinical and Toxicological Advances

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