Genotoxicity biomarkers for airborne particulate matter (PM2.5) in an area under petrochemical influence

Similar polycyclic aromatic hydrocarbon and genotoxicity profiles of atmospheric particulate matter from cities on three different continents

“…Together, results suggest that the EOM samples could induce significant levels of DNA damage but not chromosomal breaks or aneuploidy. Previous studies suggest that the negative results in the micronucleus assay indicate that the DNA damage observed in the comet was repairable (Brito et al, 2013;Lemos et al, 2016) Our results on genotoxicity with the comet assay in human lung cells agreed with the mutagenicity results in the Salmonella/microsome assay (Maselli et al, 2019) for the same EOMs, where all samples were mutagenic. Genetic damage caused by air pollution has received great attention because genotoxic pollutants are able to induce serious human diseases, including cancer (Bafana et al, 2018).…”

“…Together, these results suggest that the EOM samples could induce significant levels of DNA damage but not chromosomal breaks or aneuploidy. Previous studies suggest that the negative results in the micronucleus assay indicate that the DNA damage observed in the comet was repairable (Brito et al ., 2013; Lemos et al ., 2016). Similarly, McCarrick et al .…”

“…The former measures the ability to induce DNA damage such as strand breaks and the latter to induce chromosomal breaks or aneuploidy. Both methods have been used extensively to study the genotoxic effects of PAHs, both as single compounds and as environmental samples, including atmospheric PM (Brito et al ., 2013; Dumax‐Vorzet et al ., 2015; IARC, 2016; Lemos et al ., 2016; Long et al ., 2016; Sogbanmu et al ., 2016; Bocchi et al ., 2017; McCarrick et al ., 2019). Human bronchial epithelial cells (HBEC3‐KT) were chosen here due to their relevance for the evaluation of genotoxic effects related to inhalation exposure (Åkerlund et al ., 2018; Lebedová et al ., 2018).…”

Alzheimer and depressive cognitive-like behaviors in male and female rats: A new method for exposure to ambient air pollution

“…The association between air pollution and neuroinflammatory and neurodegenerative as one of the routes for increasing neurodegenerative disorders (ND) was approved [16,17]. However, pathophysiological mechanisms on the central nervous system (CNS) due to PM exposure is elucidated [18].…”

Estudo histológico e molecular dos efeitos da exposição ao material particulado inalável fino da cidade de São Paulo nos testículos de camundongos

“…Além de mutagenicidade, há associação de genotoxicidade relacionada à exposição ao MP2,5. Estudos já demonstraram atividade clastogênica do MP, formação de adutos e dano oxidativo de DNA (de Kok, Hogervorst et al 2005, Gualtieri, Ovrevik et al 2011, Longhin, Holme et al 2013, Lemos, Lemos et al 2016. Culturas de células alveolares e macrófagos humanos expostos ao MP2,5 apresentaram aumento na formação de 8-hydroxy-2' -deoxyguanosine (8-OHdG) -um marcador de estresse oxidativo do DNA, e de aductos no DNA (Andre, Billet et al 2011).…”