Genotoxic evaluation of two mercury compounds in the Drosophila wing spot test

Carmona, Erico R.; Kossatz, Elk; Creus, A.; Marcos, Ricard

doi:10.1016/j.chemosphere.2007.07.032

Cited by 20 publications

(8 citation statements)

References 38 publications

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“…In previous studies in vivo , MeHg decreased DNA synthesis in the brain. It altered cell cycle regulators (Burke et al , ) and reacted with SH groups of tubulins, impairing spindle function and leading to chromosomal aberrations and polyploidy (Silva‐Pereira et al , ; Carmona et al , ). Another mechanism that may contribute in DNA damage is the production of free radicals and hydrogen peroxide.…”

Section: Discussionmentioning

confidence: 99%

Cell damage through pentose phosphate pathway in fetus fibroblast cells exposed to methyl mercury

Amoli

Barin

Ebrahimi‐Rad

et al. 2011

J of Applied Toxicology

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Methylmercury (MeHg) is a global pollutant that causes malformations. There has been no direct evidence for the effect of MeHg on pentose phosphate pathway (PPP). In embryonic development, PPP is much more active. This pathway produces ribose for DNA/RNA production. It is possible that one of teratogenicity mechanisms of MeHg is through PPP. The fetus fibroblast cells were incubated with different concentrations of MeHg (0.1-100 μm). A dose-response dependence was observed in MTT assay. Transketolase activity and DNA content were determined in cell exposed to MeHg. A defect at the level of DNA content was observed. This amount of DNA was highly correlated with transketolase activity (r = 0.76). This study has demonstrated that the potential teratogenic action of MeHg is through PPP. To assess the protective effects of thiamin, the infected cells were incubated with different concentrations of thiamin. The obtained results show that thiamin pyrophosphate supplementation correlated with the toxicity. This finding confirms that thiamin therapy is suitable for the prevention of MeHg toxicity. Our study provides basic data for prevention and treatment of MeHg toxicity via boosting PPP.

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Section: Discussionmentioning

confidence: 99%

Cell damage through pentose phosphate pathway in fetus fibroblast cells exposed to methyl mercury

Amoli

Barin

Ebrahimi‐Rad

et al. 2011

J of Applied Toxicology

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“…HgCl 2 genotoxicity in rats following oral exposure was also established by comet assay (Rozgaj et al 2005). On the contrary, HgCl 2 exhibited a lack of genotoxic activity in the wing spot assay of Drosophila melanogaster (Carmona et al 2008). It was reported that the genotoxicity of mercuric compounds including HgCl 2 indicated by the CA and MN tests in peripheral blood lymphocytes could be partly due either to the disturbance of the spindle mechanism, or to the elevated level of 8-OH-dG (a product of oxidatively damaged DNA) brought by the generation of ROS.…”

Section: Discussionmentioning

confidence: 99%

Modulatory effects of Thymbra spicata L. different extracts against the mercury induced genotoxicity in human lymphocytes in vitro

2011

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Mercury, a xenobiotic metal, is a highly deleterious environmental pollutant. Moreover, in any form mercury is reported to be toxic. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, has long been investigated popularly of biological roles; mainly antimicrobial and antioxidant activities. However, there are very scarce data on the cytogenetic effects of thyme species. The purpose of this study was to investigate the genetic safety of different extracts from T. spicata (water extract, methanol extract, and ethanol extract) and the effects of T. spicata on mercury (as HgCl 2 ) induced genotoxicity. Sister chromatid exchange (SCE) and micronucleus (MN) assays were performed to assess DNA damages in cultured human lymphocytes (n = 5). Our results clearly revealed that, the SCE and MN rates induced by HgCl 2 were alleviated by the presence of T. spicata. As conclusion, this study demonstrated for the first time that the T. spicata provided increased resistance of DNA against HgCl 2 induced genetic damage in human lymphocytes. Based on the results of this study, it may be concluded that the T. spicata is a nontoxic material that could be used as a suppressor of heavy metal-induced genotoxicity.

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“…Many research studies conducted in the past have demonstrated the similarity in response to nanoparticle exposure between the fly and the mammalian model systems. This has articulated the attraction to fly model research for nanotoxicity assessment [20][21][22].…”

Section: Drosophila As a Model For Toxicity Assessmentmentioning

confidence: 99%

“…The ideal temperature is 22-24 ∘ C. Prolonged or continuous exposure to higher or lower temperature leads to altered fertility rates, impairment of viability, and distorted lifecycle durations. Fatality is recorded in unfavourable conditions [22][23][24].…”

Section: Drosophila As a Model For Toxicity Assessmentmentioning

confidence: 99%

Standardization of Alternative Methods for Nanogenotoxicity Testing inDrosophila melanogasterUsing Iron Nanoparticles: A Promising Link to Nanodosimetry

Parvathi

Rajagopal

Sumitha

2016

Journal of Nanotechnology

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The remarkable advancement of nanotechnology has triggered enormous production of metal nanoparticles and nanomaterials for diverse applications in clinical diagnostics and biomedical research. Nanotechnology has facilitated understanding and analysing nanotoxicology in a holistic approach. Iron nanoparticles have been of special interest in recent research owing to their dynamic, paramagnetic, and catalytic properties. Research studies (in vitromodel) have demonstrated the lack of toxicity in nanoiron. The present study design involvesin vivotoxicity assessment of nanoiron at specific concentrations of 0.1 mM, 1 mM, 5 mM, and 10 mM inDrosophila. DNA fragmentation assay in exposed and F1 population showed first-line toxicity to flies. Viability and reproductive ability were assessed at 24-hour and 48-hour intervals and thus indicated no statistical significance between the exposed and control groups. The wing spot assay has expressed transparent lack of toxicity in the studied concentrations of nanoiron. Protein profiling has demonstrated that the protein profiles have been intact in the larvae which confirm lack of toxicity of nanoiron. This leads to concluding that nanoiron at the defined concentrations is neither genotoxic nor mutagenic.

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Genotoxic evaluation of two mercury compounds in the Drosophila wing spot test

Cited by 20 publications

References 38 publications

Cell damage through pentose phosphate pathway in fetus fibroblast cells exposed to methyl mercury

Cell damage through pentose phosphate pathway in fetus fibroblast cells exposed to methyl mercury

Modulatory effects of Thymbra spicata L. different extracts against the mercury induced genotoxicity in human lymphocytes in vitro

Standardization of Alternative Methods for Nanogenotoxicity Testing inDrosophila melanogasterUsing Iron Nanoparticles: A Promising Link to Nanodosimetry

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