Genomic study of gene expression in the mouse lumbar spinal cord under the conditions of simulated microgravity

Islamov, R. R.; Rizvanov, Albert A.; Tyapkina, O. V.; Шенкман, Борис; Kozlovskaya, I. B.; Nikolskiy, E. E.; Grigoryev, A. I.

doi:10.1134/s0012496611040107

Cited by 6 publications

(3 citation statements)

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“…Many neurotrophic factors and neurotransmitters are also synthetized in the DRG, which exert important impacts on the nervous nutrition and injury repair. The changes of neurotrophic factor expressions and genomics and the degenerative alteration of myelin sheath occur in the fifth lumbar spine (L5) DRG in simulated microgravity, as shown previously in rats [4,5]. These aberrances illustrate the microscopic nervous damage.…”

Section: Introductionsupporting

confidence: 56%

“…Many previous studies demonstrate that astronauts are subjected to physiological and metabolic alterations and multi-organ injury in spaceflights, including bone loss and muscle atrophy, cerebral and spinal cord injury (SCI), cardiovascular, immune system disorders and so on. However, effective interventions are still lacking [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

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Oral Pyruvate Effects on Dorsal Root Ganglia in Simulated Weightlessness Rats

Zhang

Ren

et al. 2022

J Neurol Res

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Background: Long-term microgravity in spaceflight has been demonstrated with induction of multi-organ damage and dysfunction, including the dorsal root ganglia (DRG) injury. Present studies investigated protective effects of oral pyruvate in drinking water on lumbar 5 (L5) DRG tissues in rats subjected with hindlimb unweighting (HU).Methods: Sprague-Dawley male rats were randomly divided into four groups (N = 10): the control (CON) group, the suspension (SUS) group, the normal saline (SAL) group and the sodium pyruvate (PYR) group, respectively. Rats in the SUS, SAL and PYR groups were treated with simulated microgravity by HU with tail suspension for an 8-week period. Rats in the SAL and PYR groups were fed with normal saline and pyruvate saline, respectively. Histopathological hematoxylin-eosin (H&E) staining and Nissl-stained neurons as well as myelin sheath immunofluorescence examinations in L5 DRG tissues were carried out. The protein expression of glial cell line-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE) and S100 calcium-binding protein B (S100B) as well as the levels of adenosine triphosphate (ATP) and adenosine triphosphatase (ATPase) were also determined after HU procedures.Results: Data showed that nerve tissues' structure and function were robustly altered in L5 DRG of the SUS and SAL groups, whereas morphological changes were not significantly different between the PYR group and the CON group; levels of GDNF, GFAP, NSE and S100B were significantly changed in the SUS and SAL groups, but mostly preserved or overexpressed in group PYR following the HU injury. However, levels of ATP and ATPase were significantly retained, but still worse in the PYR group than in the CON group. The significance of oral pyruvate protection against DRG injury following the HU and the dose and formula of oral pyruvate solutions were discussed for the use of astronauts in space missions.Conclusions: This study indicates that oral pyruvate can effectively protect L5 DRG against the damage of pathological alterations and dysfunction induced by the HU in rats. Further investigations are needed.

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Section: Introductionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Oral Pyruvate Effects on Dorsal Root Ganglia in Simulated Weightlessness Rats

Zhang

Ren

et al. 2022

J Neurol Res

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“…A decrease in mRNA synthesis of several intracel lular signaling, cytoskeletal, and adhesive proteins and molecules of extracellular matrix was found in the lumbar spinal cord of mice, which underwent hypo gravity modulated on Earth [5]. This is indirect evi dence of the possible change in the phenotype and properties of glial cells leading to the impairment of neuron-glia interactions and further pathological changes in the morphological and functional state of motoneurons.…”

mentioning

confidence: 99%