Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

Islamov, R. R.; Gusev, Oleg; Tanabe, Aya; Terada, Masahiro; Tyapkina, O. V.; Петров, К. А.; Rizvanov, Albert A.; Kozlovskaya, I. B.; Nikolskiy, E. E.; Grigorjev, A.I.

doi:10.1016/j.actaastro.2016.01.026

Cited by 5 publications

(6 citation statements)

References 3 publications

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“…For clarity, differential performance of gene expression in mice lumbar spinal cord in three comparisons is presented in the form of volcano plots ( Figure 2 ). It is important to note that multiple groups comparison results in decrease of statistical power which did not reveal differentially expressed genes between S and C groups, as was shown earlier (Islamov et al, 2016). Full lists of genes considered as differentially expressed with appropriate statistics are presented in Table S2 .…”

Section: Resultssupporting

confidence: 51%

“…In comparison with group C, out of genes declared on the microarray platform increased and decreased expressions were documented in 134 genes and 41 genes, respectively. The list of up- and downregulated genes is published by Islamov R. and coauthors (Islamov et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…The microarray analysis procedures for group R were performed as described previously for groups S and C (Islamov et al, 2016). In short, total RNA was extracted from the entire sample of lumbar enlargements using the RNeasy Mini Kit (Qiagen, Valencia, CA).…”

Section: Methodsmentioning

confidence: 99%

“…To test the hypothesis that changes in spinal motor neurons provoke HMS, a full genome study of the mice lumbar spinal cord was performed after the 30-day Bion-M1 mission. The list of genes from the lumbar spine of mice with significant increases and decreases has been presented in our previous study (Islamov et al, 2016). Some changes in gene expression supported our hypothesis that molecular changes in spinal motor neurons are among the key factors in HMS pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Bioinformatic Study of Transcriptome Changes in the Mice Lumbar Spinal Cord After the 30-Day Spaceflight and Subsequent 7-Day Readaptation on Earth: New Insights Into Molecular Mechanisms of the Hypogravity Motor Syndrome

et al. 2019

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The hypogravity motor syndrome (HMS) is one of the deleterious impacts of weightlessness on the human body in orbital space missions. There is a hypothesis that disorders of musculoskeletal system as part of HMS arise in consequence of changes in spinal motor neurons. The study was aimed at bioinformatic analysis of transcriptome changes in lumbar spinal cords of mice after a 30-day spaceflight aboard biosatellite Bion-M1 (space group, S) and subsequent 7-day readaptation to the Earth’s gravity (recovery group, R) when compared with control mice (C group) housed in simulated biosatellite conditions on the Earth. Gene ontology and human phenotype ontology databases were used to detect biological processes, molecular functions, cellular components, and human phenotypes associated with HMS. Our results suggest resemblance of molecular changes developing in space orbit and during the postflight recovery to terrestrial neuromuscular disorders. Remarkably, more prominent transcriptome changes were revealed in R vs. S and R vs. C comparisons that are possibly related to the 7-day recovery period in the Earth’s gravity condition. These data may assist with establishment of HMS pathogenesis and proposing effective preventive and therapeutic options.

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Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioinformatic Study of Transcriptome Changes in the Mice Lumbar Spinal Cord After the 30-Day Spaceflight and Subsequent 7-Day Readaptation on Earth: New Insights Into Molecular Mechanisms of the Hypogravity Motor Syndrome

et al. 2019

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“…To test the hypothesis that changes in spinal motor neurons provoke HMS, researchers performed a full genome study of the mice lumbar spinal cord after the 30-day 'Bion-M1' mission [23]. Data published to date generally provide evidence for the spinal cord motoneuron functional plasticity under altered gravitational forces.…”

Section: Introductionmentioning

confidence: 99%

Cytoskeleton Markers in the Spinal Cord and Mechanoreceptors of Thick-Toed Geckos after Prolonged Space Flights

Proshchina

Gulimova

Kharlamova

et al. 2022

Life

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Spaceflight may cause hypogravitational motor syndrome (HMS). However, the role of the nervous system in the formation of HMS remains poorly understood. The aim of this study was to estimate the effects of space flights on the cytoskeleton of the neuronal and glial cells in the spinal cord and mechanoreceptors in the toes of thick-toed geckos (Chondrodactylus turneri GRAY, 1864). Thick-toed geckos are able to maintain attachment and natural locomotion in weightlessness. Different types of mechanoreceptors have been described in the toes of geckos. After flight, neurofilament 200 immunoreactivity in mechanoreceptors was lower than in control. In some motor neurons of flight geckos, nonspecific pathomorphological changes were observed, but they were also detected in the control. No signs of gliosis were detected after spaceflight. Cytoskeleton markers adequately reflect changes in the cells of the nervous system. We suggest that geckos’ adhesion is controlled by the nervous system. Our study revealed no significant disturbances in the morphology of the spinal cord after the prolonged space flight, supporting the hypothesis that geckos compensate the alterations, characteristic for other mammals in weightlessness, by tactile stimulation.

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Mice display learning and behavioral deficits after a 30-day spaceflight on Bion-M1 satellite

Andreev-Andrievskiy

Dolgov

Alberts

et al. 2022

Behavioural Brain Research

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Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

Cited by 5 publications

References 3 publications

Bioinformatic Study of Transcriptome Changes in the Mice Lumbar Spinal Cord After the 30-Day Spaceflight and Subsequent 7-Day Readaptation on Earth: New Insights Into Molecular Mechanisms of the Hypogravity Motor Syndrome

Bioinformatic Study of Transcriptome Changes in the Mice Lumbar Spinal Cord After the 30-Day Spaceflight and Subsequent 7-Day Readaptation on Earth: New Insights Into Molecular Mechanisms of the Hypogravity Motor Syndrome

Cytoskeleton Markers in the Spinal Cord and Mechanoreceptors of Thick-Toed Geckos after Prolonged Space Flights

Mice display learning and behavioral deficits after a 30-day spaceflight on Bion-M1 satellite

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