Genomic identification and characterization of MYC family genes in wheat (Triticum aestivum L.)

Abstract

BackgroundMYC transcriptional factors are members of the bHLH (basic helix-loop-helix) superfamily, and play important roles in plant growth and development. Recent studies have revealed that some MYCs are involved in the crosstalk between Jasmonic acid regulatory pathway and light signaling in Arabidopsis, but such kinds of studies are rare in wheat, especially in photo-thermo-sensitive genic male sterile (PTGMS) wheat line.Results27 non-redundant MYC gene copies, which belonged to 11 TaMYC genes, were identified in the whole genome of wheat (Chinese Spring). These gene copies were distributed on 13 different chromosomes, respectively. Based on the results of phylogenetic analysis, 27 TaMYC gene copies were clustered into group I, group III, and group IV. The identified TaMYC genes copies contained different numbers of light, stress, and hormone-responsive regulatory elements in their 1500 base pair promoter regions. Besides, we found that TaMYC3 was expressed highly in stem, TaMYC5 and TaMYC9 were expressed specially in glume, and the rest of TaMYC genes were expressed in all tissues (root, stem, leaf, pistil, stamen, and glume) of the PTGMS line BS366. Moreover, we found that TaMYC3, TaMYC7, TaMYC9, and TaMYC10 were highly sensitive to methyl jasmonate (MeJA), and other TaMYC genes responded at different levels. Furthermore, we confirmed the expression profiles of TaMYC family members under different light quality and plant hormone stimuli, and abiotic stresses. Finally, we predicted the wheat microRNAs that could interact with TaMYC family members, and built up a network to show their integrative relationships.ConclusionsThis study analyzed the size and composition of the MYC gene family in wheat, and investigated stress-responsive and light quality induced expression profiles of each TaMYC gene in the PTGMS wheat line BS366. In conclusion, we obtained lots of important information of TaMYC family, and the results of this study was supposed to contribute novel insights and gene and microRNA resources for wheat breeding, especially for the improvement of PTGMS wheat lines.

Genome Wide Identification and Characterization of Wheat GH9 Genes Reveals Their Roles in Pollen Development and Anther Dehiscence

“…The remaining 63 copies were all fragmental replications, suggesting that fragmental replication was associated with the evolution of the wheat GH9 family and the expansion of members. The findings indicated that fragmental replication may be the main driver of the wheat GH9 gene family expansion, in line with previous research [ 29 ].…”

“…Hormone-responsive elements are enriched in these TaGH9s , especially MeJA response elements (TGACG-motif and CGTCA-motif) and abscisic acid response elements (ABRE element) are enriched in the TaGH9 family ( Figure 4 ). The latest research has informed us that GA and JA signaling are associated with anther dehiscence in BS366 [ 29 , 37 ]. Phytohormone crosstalk as a massive network has been known to be involved in plant growth.…”

Expression profiling of the Dof gene family under abiotic stresses in spinach

“…At the C-terminal of the Dof proteins, there is a transcription regulation domain with diverse functions involving interaction with a variety of regulatory proteins and activating the gene expression 15 . Indeed, previous studies corroborated its functional role in plant growth and development, such as in flowering control 16 , 17 , maturation 18 , seed development 19 , and germination 20 , 21 . Specifically, mutant dag1 (encoding a Dof transcription factor in Arabidopsis) seeds are induced to germinate by much lower red light fluence rates 22 ; the COG1 gene (encoding a Dof protein in Arabidopsis) functions as a negative regulator in phytochrome signaling pathways 23 ; CDFs (CYCLING DOF FACTORS, Dof-type transcriptional repressors) that directly suppresses the expression of CONSTANS ( CO ), which could prevent the expression of photoperiodic gene, the perception of day-length and the floral transition in Arabidopsis 24 .…”

Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances

“…In order to name all wheat WRKY genes sequentially, we utilized a consistent naming pattern based on their phylogenetic relationships and chromosome locations in A, B, and D subgenomes (Bai et al, 2019). First, each name of the wheat WRKY genes was started with the abbreviation of the species name Triticum aestivum (Ta).…”