Genome-wide phylogenetic analysis of differences in thermotolerance among closely related Acetobacter pasteurianus strains

Abstract

Acetobacter pasteurianus is a Gram-negative strictly aerobic bacterium that is widely used for the industrial production of vinegar. Three Acetobacter pasteurianus strains, SKU1108, NBRC 3283 and IFO 3191, have the same 16S rRNA sequence (100 % sequence identity) but show differences in thermotolerance. To clarify the relationships between phylogeny and thermotolerance of these strains, genome-wide analysis of these three strains was performed. Concatenated phylogenetic analysis of a dataset of 1864 orthologues has shown that the more thermotolerant strains, SKU1108 and NBRC 3283, are more closely related to each other than to the more thermosensitive strain, IFO 3191. In addition, we defined a dataset of 2010 unique orthologues among these three strains, and compared the frequency of amino acid mutations among them. Genes involved in translation, transcription and signal transduction are highly conserved among each unique orthologous dataset. The results also showed that there are several genes with increased mutation rates in IFO 3191 compared with the thermotolerant strains, SKU1108 and NBRC 3283. Analysis of the mutational directions of these genes suggested that some of them might be correlated with the thermosensitivity of IFO 3191. Concatenated phylogenetic analysis of these closely related strains revealed that there is a phylogenetic relationship associated with this phenotype among the thermotolerant and thermosensitive strains.

Protein Profile of Acetobacter pasteurianus HSZ3-21

“…In addition, ABC transporter proteins and transporter-related proteins were identified in this study (spot nos. 38, 157, 173, 194), which are involved in transport systems and might be responsible for the AcOH tolerance and thermotolerance [10,14].…”

“…Previous studies suggested that OMPs (OmpA, OmpW) might be related to the resistance to AcOH and thermotolerance [1,10]. Heat shock proteins (HSPs) are crucial in the inherent folding of proteins under normal growth conditions and can protect the proteins from aggregation and denaturation caused by heat or other environmental stresses [1,10,12]. In this study, HSPs and HSPs-related protein, including DnaK (Hsp70), HtpG (Hsp90), GroEL (Hsp60), HspA (Hsp20/alpha), Clp protease ATP-binding subunit ClpB, ATP-dependent Clp protease proteolytic subunit, and endopeptidase DegP (spot nos.…”

“…108,111,137,195,222) were also identified in this study. Previous studies suggested that OMPs (OmpA, OmpW) might be related to the resistance to AcOH and thermotolerance [1,10]. Heat shock proteins (HSPs) are crucial in the inherent folding of proteins under normal growth conditions and can protect the proteins from aggregation and denaturation caused by heat or other environmental stresses [1,10,12].…”

“…Recent decade has witnessed growing interest in studies of A. pasteurianus, such as A. pasteurianus isolation and identification from different vinegar fermentations, the strain improvement of A. pasteurianus, and the physiological characteristics research of A. pasteurianus in the acetification process [10,17,19]. Compared with thermosensitive strain IFO 3191 using genome-wide phylogenetic analysis, several genes in thermotolerant strains SKU 1108 and NBRC 3283 are mainly involved in stress response, cell wall or cell membrane biosynthesis, and transport systems [10], suggesting that A. pasteurianus do have certain resistance mechanism toward stress conditions. Further study revealed the underlying mechanisms of its metabolic features and tolerance toward stress conditions by genome analysis of A. pasteurianus 386B [7].…”

Expression of Gene uvrA from Acetobacter pasteurianus and Its Tolerance to Acetic Acid in Escherichia coli

“…As its important application value, the whole-genome of A. pasteurianus has been sequenced completely [9,10]. This research is focused on the repair excinuclease UvrA from A. pasteurianus AC2005.…”