Expression of Gene uvrA from Acetobacter pasteurianus and Its Tolerance to Acetic Acid in Escherichia coli

Abstract

The uvrA gene of Acetobacter pasteurianus AC2005 coding for subunit A of the excinuclease ABC complex involved in the nucleotide excision repair mechanism was identified. Gene uvrA was amplified using A. pasteurianus AC2005 genomic DNA as a template. Then the pMV24 plasmid, an expression vector of Acetobacter, was used for constructing the recombinant plasmid pMV24-uvrA. UvrA was expressed in Escherichia coli JM109, and its molecular weight was about 91.1 kDa. With 0.5 % acetic acid shock for 20 and 40 min, the survival rates of recombinant strain E. coli JM109/pMV24-uvrA were 0.48 and 0.056 %, which increased by 17.5 and 10.2 times, respectively, compared with those of E. coli JM109/pMV24. All these demonstrate that the expression of repair excinuclease UvrA could increase the acetic acid tolerance of the strain.