Genome-Wide Identification of MicroRNAs in Response to Cadmium Stress in Oilseed Rape (Brassica napus L.) Using High-Throughput Sequencing

Jian, Hongju; Yang, Bo; Zhang, Aoxiang; Ma, Jinqi; Ding, Yiran; Chen, Zhiyou; Li, Jiana; Xu, Xinhua; Liu, Liezhao

doi:10.3390/ijms19051431

Cited by 39 publications

(33 citation statements)

References 64 publications

(80 reference statements)

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“…In a recent study using a plant model, Jian et al, demonstrated that exposure to Cd for up to 3 days resulted in 39 differentially expressed miRNAs that were involved in homeostasis, stress response, transcription factor regulation, and secondary cellular metabolic responses [46]. Consequently, the ingestion of contaminated plants will result in higher concentration and bioaccumulation of Cd that contributes to increased Cd body burden.…”

Section: Cadmium-associated Changes In Mirna Expressionmentioning

confidence: 99%

Toxic-Metal-Induced Alteration in miRNA Expression Profile as a Proposed Mechanism for Disease Development

Wallace

Taalab

Heinze

et al. 2020

Cells

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Toxic metals are extensively found in the environment, households, and workplaces and contaminate food and drinking water. The crosstalk between environmental exposure to toxic metals and human diseases has been frequently described. The toxic mechanism of action was classically viewed as the ability to dysregulate the redox status, production of inflammatory mediators and alteration of mitochondrial function. Recently, growing evidence showed that heavy metals might exert their toxicity through microRNAs (miRNA)-short, single-stranded, noncoding molecules that function as positive/negative regulators of gene expression. Aberrant alteration of the endogenous miRNA has been directly implicated in various pathophysiological conditions and signaling pathways, consequently leading to different types of cancer and human diseases. Additionally, the gene-regulatory capacity of miRNAs is particularly valuable in the brain-a complex organ with neurons demonstrating a significant ability to adapt following environmental stimuli. Accordingly, dysregulated miRNAs identified in patients suffering from neurological diseases might serve as biomarkers for the earlier diagnosis and monitoring of disease progression. This review will greatly emphasize the effect of the toxic metals on human miRNA activities and how this contributes to progression of diseases such as cancer and neurodegenerative disorders (NDDs).

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Section: Cadmium-associated Changes In Mirna Expressionmentioning

confidence: 99%

Toxic-Metal-Induced Alteration in miRNA Expression Profile as a Proposed Mechanism for Disease Development

Wallace

Taalab

Heinze

et al. 2020

Cells

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“…Solanum tuberosum L. under light stimulus found light-responsive miRNAs that are important regulators in alkaloid metabolism, UMP salvage, lipid biosynthesis, and cellulose catabolism [24]. Cadmium stress in oilseed rape (Brassica napus L.) reported miRNAs involved in the regulations of TFs, biotic stress defense, ion homeostasis, and secondary metabolism synthesis [25]. Nicotiana tabacum plants infected with tobacco mosaic virus (TMV), at the early stage of infection (5 dpi), show a cluster of miRNAs with down-accumulation, while most of the miRNAs were upregulated at 15 and 22 dpi, including both miRNAs and miRNA targets [26].…”

Section: Definition Of Mirnamentioning

confidence: 99%

MicroRNAs Associated with Secondary Metabolites Production

Vargas-Hernández¹,

Vázquez-Marrufo²,

Agustín³

et al. 2019

Plant Physiological Aspects of Phenolic Compounds

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MicroRNAs (miRNAs) are noncoding RNAs that play an important role in the regulation of the genetic expression in animals and plants by targeting mRNAs for cleavage or translational repression. Several miRNAs regulate the plant development, the metabolism, and the responses to biotic and abiotic stresses. Characterization of an miRNA has helped to show its role in fine tuning the mechanisms of posttranscriptional gene regulation. Although there is a lot of information related to miRNA regulation of some processes, the role of miRNA involved in the regulation of biosynthesis of secondary plant product is still poorly understood. In this chapter, we summarize the identification and characterization of miRNAs that participate in the regulation of the biosynthesis of secondary metabolites in plants and their use in the strategies to manipulate a controlled manipulation.

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“…They have been implicated in abiotic stress tolerance (Shriram et al 2016), including the response to heavy metal stress in plants (Noman and Aqeel, 2017). Some Cd responsive miRNAs have been identified in rice (Wang et al 2009;Ding et al 2011), B. napus (Zhou et al 2012a;Jian et al 2018), B. parachinensis (Zhou et al 2017), soybean (Fang et al 2013) and the hyper-accumulator Sedum alfredii (Han et al 2016). Additionally, specific functions of miR166 and miR395 in Cd accumulation and tolerance have been reported in rice and B. napus, respectively (Zhang et al 2013;Ding et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Cultivar- and tissue-specific transcriptomic changes induced by cadmium in Brassica parachinensis

Liu

Deng

et al. 2019

Preprint

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Background One of the main pathways for cadmium (Cd) transfer from the environment to the human body is through the consumption of leafy vegetables, and Brassica leafy crops tend to be Cd hyper-accumulators. But its response strategies to Cd still lack of systematic study. Results To investigate Brassica response strategies to Cd, we identified two cultivars with different Cd translocation efficiencies and performed mutli-transcriptomic sequencing studies under Cd treatments. Certain transporter families exhibited different temporal expression profiles in the two cultivars and may underlie the different Cd translocation efficiencies. Cd induced a drastic reduction of a 22 nt small RNA, the footprint of a pentatricopeptide repeat (PPR) protein on the chloroplast ndhB transcript and the concomitant down-regulation of the ndhB transcript. A global reduction in the expression of PPR genes was found, revealing previously unknown effects of Cd on organellar gene expression. Analyses of microRNAs (miRNAs) and their target genes by small RNA and degradome sequencing not only revealed Cd-induced changes in miRNAs but also implicated the existence of a regulatory cascade involving bra-miR156, its target gene, and bra-miR397 and bra-miR398 in Cd stress responses. Conclusions The present findings help uncover the impact of Cd stress on the transcriptome of B. parachinensis and provide candidate genes and miRNAs for further investigation.

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Genome-Wide Identification of MicroRNAs in Response to Cadmium Stress in Oilseed Rape (Brassica napus L.) Using High-Throughput Sequencing

Cited by 39 publications

References 64 publications

Toxic-Metal-Induced Alteration in miRNA Expression Profile as a Proposed Mechanism for Disease Development

Toxic-Metal-Induced Alteration in miRNA Expression Profile as a Proposed Mechanism for Disease Development

MicroRNAs Associated with Secondary Metabolites Production

Cultivar- and tissue-specific transcriptomic changes induced by cadmium in Brassica parachinensis

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