The basic region/leucine zipper motif (bZIP) transcription factor family is one of the largest families of transcriptional regulators in plants. bZIP genes have been systematically characterized in some plants, but not in rapeseed (Brassica napus). In this study, we identified 247 BnbZIP genes in the rapeseed genome, which we classified into 10 subfamilies based on phylogenetic analysis of their deduced protein sequences. The BnbZIP genes were grouped into functional clades with Arabidopsis genes with similar putative functions, indicating functional conservation. Genome mapping analysis revealed that the BnbZIPs are distributed unevenly across all 19 chromosomes, and that some of these genes arose through whole-genome duplication and dispersed duplication events. All expression profiles of 247 bZIP genes were extracted from RNA-sequencing data obtained from 17 different B. napus ZS11 tissues with 42 various developmental stages. These genes exhibited different expression patterns in various tissues, revealing that these genes are differentially regulated. Our results provide a valuable foundation for functional dissection of the different BnbZIP homologs in B. napus and its parental lines and for molecular breeding studies of bZIP genes in B. napus.
BackgroundOptimum flowering time is a key agronomic trait in Brassica napus. To investigate the genetic architecture and genetic regulation of flowering time in this important crop, we conducted quantitative trait loci (QTL) analysis of flowering time in a recombinant inbred line (RIL) population, including lines with extreme differences in flowering time, in six environments, along with RNA-Seq analysis.ResultsWe detected 27 QTLs distributed on eight chromosomes among six environments, including one major QTL on chromosome C02 that explained 11–25% of the phenotypic variation and was stably detected in all six environments. RNA-Seq analysis revealed 105 flowering time-related differentially expressed genes (DEGs) that play roles in the circadian clock/photoperiod, autonomous pathway, and hormone and vernalization pathways. We focused on DEGs related to the regulation of flowering time, especially DEGs in QTL regions.ConclusionsWe identified 45 flowering time-related genes in these QTL regions, eight of which are DEGs, including key flowering time genes PSEUDO RESPONSE REGULATOR 7 (PRR7) and FY (located in a major QTL region on C02). These findings provide insights into the genetic architecture of flowering time in B. napus.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5356-8) contains supplementary material, which is available to authorized users.
Sucrose is the principal transported product of photosynthesis from source leaves to sink organs. SUTs/SUCs (sucrose transporters or sucrose carriers) and SWEETs (Sugars Will Eventually be Exported Transporters) play significant central roles in phloem loading and unloading. SUTs/SUCs and SWEETs are key players in sucrose translocation and are associated with crop yields. The SUT/SUC and SWEET genes have been characterized in several plant species, but a comprehensive analysis of these two gene families in oilseed rape has not yet been reported. In our study, 22 and 68 members of the SUT/SUCs and SWEET gene families, respectively, were identified in the oilseed rape (Brassica napus) genome through homology searches. An analysis of the chromosomal distribution, phylogenetic relationships, gene structures, motifs and the cis-acting regulatory elements in the promoters of BnSUC and BnSWEET genes were analyzed. Furthermore, we examined the expression of the 18 BnSUC and 16 BnSWEET genes in different tissues of “ZS11” and the expression of 9 BnSUC and 7 BnSWEET genes in “ZS11” under various conditions, including biotic stress (Sclerotinia sclerotiorum), abiotic stresses (drought, salt and heat), and hormone treatments (abscisic acid, auxin, cytokinin, brassinolide, gibberellin, and salicylic acid). In conclusion, our study provides the first comprehensive analysis of the oilseed rape SUC and SWEET gene families. Information regarding the phylogenetic relationships, gene structure and expression profiles of the SUC and SWEET genes in the different tissues of oilseed rape helps to identify candidates with potential roles in specific developmental processes. Our study advances our understanding of the important roles of sucrose transport in oilseed rape.
Glutathione transferases (GSTs) are multifunctional enzymes that play important roles in plant development and responses to biotic and abiotic stress. However, a systematic analysis of GST family members in Brassica napus has not yet been reported. In this study, we identified 179 full-length GST genes in B . napus , 44.2% of which are clustered on various chromosomes. In addition, we identified 141 duplicated GST gene pairs in B . napus . Molecular evolutionary analysis showed that speciation and whole-genome triplication played important roles in the divergence of the B . napus GST duplicated genes. Transcriptome analysis of 21 tissues at different developmental stages showed that 47.6% of duplicated GST gene pairs have divergent expression patterns, perhaps due to structural divergence. We constructed a GST gene coexpression network with genes encoding various transcription factors (NAC, MYB, WRKY and bZIP) and identified six modules, including genes expressed during late seed development (after 40 days; BnGSTU19 , BnGSTU20 and BnGSTZ1 ) and in the seed coat ( BnGSTF6 and BnGSTF12 ), stamen and anther ( BnGSTF8 ), root and stem ( BnGSTU21 ), leaves and funiculus, as well as during the late stage of pericarp development (after 40 days; BnGSTU12 and BnGSTF2 ) and in the radicle during seed germination ( BnGSTF14 , BnGSTU1 , BnGSTU28 , and BnGSTZ1 ). These findings lay the foundation for elucidating the roles of GSTs in B . napus .
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