Genome-Wide Identification, Expression Pattern Analysis and Evolution of the Ces/Csl Gene Superfamily in Pineapple (Ananas comosus)

Abstract

The cellulose synthase (Ces) and cellulose synthase-like (Csl) gene families belonging to the cellulose synthase gene superfamily, are responsible for the biosynthesis of cellulose and hemicellulose of the plant cell wall, and play critical roles in plant development, growth and evolution. However, the Ces/Csl gene family remains to be characterized in pineapple, a highly valued and delicious tropical fruit. Here, we carried out genome-wide study and identified a total of seven Ces genes and 25 Csl genes in pineapple. Genomic features and phylogeny analysis of Ces/Csl genes were carried out, including phylogenetic tree, chromosomal locations, gene structures, and conserved motifs identification. In addition, we identified 32 pineapple AcoCes/Csl genes with 31 Arabidopsis AtCes/Csl genes as orthologs by the syntenic and phylogenetic approaches. Furthermore, a RNA-seq investigation exhibited the expression profile of several AcoCes/Csl genes in various tissues and multiple developmental stages. Collectively, we provided comprehensive information of the evolution and function of pineapple Ces/Csl gene superfamily, which would be useful for screening out and characterization of the putative genes responsible for tissue development in pineapple. The present study laid the foundation for future functional characterization of Ces/Csl genes in pineapple.

Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars

“…A genome-wide analysis of gene family is an efficient approach for understanding gene structure, function, and evolution. To date, detailed genome-wide explorations of Csls have been limited to less than 20 plant species, such as Arabidopsis, poplar, rice, sorghum ( Sorghum bicolor ), maize, and various grasses [ 50 , 53 , 56 , 57 , 59 , 64 , 65 ]. In this study, we conducted a comprehensive analysis of the MaCsl gene family, including the identification of members, phylogenetic relationships, chromosomal distribution, and expression profiles in two LT-treated banana cultivars with different tolerances to LT. A total of 42 putative Csl genes were identified in the Musa acuminata genome.…”

“…A detailed characterization of the plant Csl genes will be helpful for better understanding their functional and biochemical properties. To date, the whole Csl gene repertoire has been cataloged in rice ( Oryza sativa ) [ 13 ], poplar ( Populus trichocarpa ) [ 49 , 50 ], moss ( Physcomitrella patens ) [ 51 ], maize ( Zea mays ) [ 52 , 53 ], barley ( Hordeum vulgare ) [ 54 ], pine ( Pinus taed ) [ 55 ], tomato ( Solanum lycopersicum ) [ 56 ], pineapple ( Ananas comosus ) [ 57 ], bread wheat ( Triticum aestivum L.) [ 58 ] and white pears ( Pyrus bretschneideri ) [ 59 ]. However, these genes have not been extensively studied in banana ( Musa spp.…”

Bioinformatics Analysis of Cellulose Synthase <i>CesA</i> Gene from <i>Miscanthus lutarioriparius</i>

“…In Hordeum vulgare, the small RNA produced by HvCesA6 can selectively attenuate the expression of CesA gene, and this expression may affect the expression of other cell wall biosynthesis genes, thereby greatly affecting the content of barley cellulose ( Michael et al, 2008). In the plant cellulose synthesis pathway, in addition to CesA gene, the expression and mutation of CSL gene (Cao et al, 2019), KORRIGAN gene (Aggarwa et al, 2015), COBRA gene (Et al., 2020) and SUSY gene (Ahmed et al, 2019) in plants can also affect the synthesis of cellulose.…”

Genome-wide identification of Cellulose-like synthase D gene family in Dendrobium catenatum

“…Studies have shown that three CslA genes (CslA2, CslA7 and CslA9) and three CslD genes (CslD2, CslD3 and CslD5) participate in the synthesis of mannose-containing polysaccharides in Arabidopsis thaliana [16,17]. CslC genes are responsible for the biosynthesis of xyloglucan in Ananas comosus [18,19]. CslF and CslH gene families could mediate the synthesis of (1,3;1,4)-β-glucan, which is a polysaccharide characteristic of the evolutionarily successful grasses that is not widely distributed elsewhere in the plant kingdom [20][21][22].…”