Genome-Wide Identification of Banana Csl Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars

Abstract

The cell wall plays an important role in responses to various stresses. The cellulose synthase-like gene (Csl) family has been reported to be involved in the biosynthesis of the hemicellulose backbone. However, little information is available on their involvement in plant tolerance to low-temperature (LT) stress. In this study, a total of 42 Csls were identified in Musa acuminata and clustered into six subfamilies (CslA, CslC, CslD, CslE, CslG, and CslH) according to phylogenetic relationships. The genomic features of MaCsl genes were characterized to identify gene structures, conserved motifs and the distribution among chromosomes. A phylogenetic tree was constructed to show the diversity in these genes. Different changes in hemicellulose content between chilling-tolerant and chilling-sensitive banana cultivars under LT were observed, suggesting that certain types of hemicellulose are involved in LT stress tolerance in banana. Thus, the expression patterns of MaCsl genes in both cultivars after LT treatment were investigated by RNA sequencing (RNA-Seq) technique followed by quantitative real-time PCR (qPCR) validation. The results indicated that MaCslA4/12, MaCslD4 and MaCslE2 are promising candidates determining the chilling tolerance of banana. Our results provide the first genome-wide characterization of the MaCsls in banana, and open the door for further functional studies.

Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut

“…Among the “biological process” category, the most significantly enriched terms are “response to biotic stimulus” and “defense response.” In addition, the “metal ion transport” term included 14 genes encoding for heavy metal-associated isoprenylated plant proteins (HIPPs), which were reported to play important roles in response to biotic/abiotic stresses ( Zhang et al, 2015 ). The top enriched GO terms in “molecular functions” were related to “transferring acyl groups” and “glucosyltransferase activity.” Of these terms, 12 genes were annotated as putative cellulose synthases involved in the biosynthesis of the hemicellulose backbone, which might be helpful to confer abiotic stress tolerance to plants ( Endler et al, 2015 ; Huang et al, 2021 ; Yuan et al, 2021 ).…”

“…The top enriched GO terms in "molecular functions" were related to "transferring acyl groups" and "glucosyltransferase activity." Of these terms, 12 genes were annotated as putative cellulose synthases involved in the biosynthesis of the hemicellulose backbone, which might be helpful to confer abiotic stress tolerance to plants (Endler et al, 2015;Huang et al, 2021;Yuan et al, 2021).…”

Physio-biochemical and molecular stress regulators and their crosstalk for low-temperature stress responses in fruit crops: A review

“…The results provided background information on MaFLAs and suggested their involvement in plant chilling tolerance and their potential as candidate genes to be targeted when breeding for cold tolerance. Yuan et al (2021) reported that the Csl (Cellulose synthase-like) gene family imparts tolerance against LT in bananas by synthesizing a special kind of hemicellulose, an essential component of the cell wall, and strengthening the stiffness of the cell wall. The expression of genes MaCslD4 (Musa acuminate Cellulose synthase-like gene), MaCslA4/12, and MaCslE2 (member of the Csl gene family) were found to be significantly higher in the tolerant variety than in the susceptible banana variety under LTS (Yuan et al, 2021).…”

Genome-wide identification and functional analysis of Cellulose synthase gene superfamily in Fragaria vesca

“…The CesA and Csl gene families associated with cell wall polysaccharide biosynthesis play important roles in plant growth and development and responses to environmental stress ( McFarlane et al., 2014 ). To date, the CesA/Csl gene families have been extensively identified and studied in various species, such as Arabidopsis ( Persson et al., 2007 ), rice ( Hazen et al., 2002 ), cotton ( Pear et al., 1996 ) and banana ( Yuan et al., 2021 ), but they have not been reported previously in strawberry. In this study, eight FveCesA genes and 25 FveCsl genes were identified in diploid woodland strawberry by genome-wide sequence analysis.…”