Genome-Wide Identification and Characterization of the Calmodulin-Binding Transcription Activator (CAMTA) Gene Family in Plants and the Expression Pattern Analysis of CAMTA3/SR1 in Tomato under Abiotic Stress

Abstract

Calmodulin-binding transcription activator (CAMTA) plays an important regulatory role in plant growth, development, and stress response. This study identified the phylogenetic relationships of the CAMTA family in 42 plant species using a genome-wide search approach. Subsequently, the evolutionary relationships, gene structures, and conservative structural domain of CAMTA3/SR1 in different plants were analyzed. Meanwhile, in the promoter region, the cis-acting elements, protein clustering interaction, and tissue-specific expression of CAMTA3/SR1 in tomato were identified. The results show that SlCAMTA3/SR1 genes possess numerous cis-acting elements related to hormones, light response, and stress in the promoter regions. SlCAMTA3 might act together with other Ca2+ signaling components to regulate Ca2+-related biological processes. Then, the expression pattern of SlCAMTA3/SR1 was also investigated by quantitative real-time PCR (qRT-PCR) analysis. The results show that SlCAMTA3/SR1 might respond positively to various abiotic stresses, especially Cd stress. The expression of SlCAMTA3/SR1 was scarcely detected in tomato leaf at the seedling and flowering stages, whereas SlCAMTA3/SR1 was highly expressed in the root at the seedling stage. In addition, SlCAMTA3/SR1 had the highest expression levels in flowers at the reproductive stage. Here, we provide a basic reference for further studies about the functions of CAMTA3/SR1 proteins in plants.

Identification and Bioinformatic Analysis of CAMTA Genes in Olive (Olea europaea L.)

“…Özbilen / Uluslararası Fen Araştırmalarında Yenilikçi Yaklaşımlar Dergisi / International Journal of Innovative Approaches in Science Research, 2021, Vol. 6 (2), 64-78 74 Linum usitatissimum (Ali et al, 2020), 18 for Oryza sativa (Gain et al, 2022) and 7 for Solanum lycopersicum (Fang et al, 2022).…”

“…Thanks to genome sequencing and bioinformatic tools, many CAMTAs were identified in various plant species. For the phylogenetic analysis, CAMTA protein sequences of A. thaliana (Yang and Poovaiah, 2002), Citrus species (Zhang et al, 2019), Glycine max (Wang et al, 2015), Linum usitatissimum (Ali et al, 2020), Nicotiana tabacum (Kakar et al,2018), Oryza sativa (Gain et al, 2022), Phaseolus vulgaris (Büyük et al, 2019), Populus trichocarpa (Wei et al, 2017), Solanum lycopersicum (Fang et al, 2022) and Vitis vinifera (Shangguan et al, 2014) were downloaded from NCBI (National Center for Biotechnology Information, https://www.ncbi.nlm.nih.gov/) and from Phytozome v13 (https://phytozome-next.jgi.doe.gov/) databases. These sequences were aligned with olive sequences using MUSCLE algorithm (Edgar, 2004) in Geneious software with default parameters.…”

“…Since CAMTAs are first discovered in tobacco (Yang and Poovaiah, 2000), this gene family is studied widely in numerous plant species such as: Arabidopsis thaliana (Yang and Poovaiah, 2002), Vitis vinifera (Shangguan et al, 2014), Glycine max (Wang et al, 2015), Populus trichocarpa (Wei et al, 2017), Citrus species (Zhang et al, 2019), Phaseolus vulgaris (Büyük et al, 2019), Linum usitatissimum (Ali et al, 2020), Oryza sativa (Gain et al, 2022) and Solanum lycopersicum (Fang et al, 2022). CAMTAs are found to be involved in lots of biological process like drought (Pandey et al, 2013;Kakar et al, 2018;Zhang et al, 2019), cold (Doherty et al, 2009;Kim et al, 2013;Wei et al, 2017;Kakar et al, 2018;Primo-Capella et al, 2021), salt stress (Wei et al, 2017;Büyük et al, 2019;Zhang et al, 2019;Yuan et al, 2021), wounding or pathogenic invasions (Nie et al, 2012;Wei et al, 2017;Kakar et al, 2018), hormone signaling (Wei et al, 2017;Zhang et al, 2019), ethylene signaling and fruit ripening (Yang et al, 2012).…”

Identification and Molecular Characterization of the CAMTA Gene Family in Solanaceae with a Focus on the Expression Analysis of Eggplant Genes under Cold Stress

“…It has been reported that the CAMTA family is involved in fruit development and abiotic stress processes in tomato [46], soybean [26], and other plants [52]. In tomato, for example, SlCAMTA3 expression is scarce in leaves in the seedling and flowering stages, while it is highly expressed in roots in the seedling stage [53]. In the case of eggplant, the expression of the SmCAMTA3 and SmCAMTA4 genes is highly observed in the leaves but is lowly detected in eggplant flowers (Figure 5A).…”

Comprehensive identification and expression analysis of CAMTA gene family in Phyllostachys edulis under abiotic stress

“…During signal transduction, multiple cis-acting elements on a gene promoter work together to regulate multiple complex biological responses. Solanum lycopersicum CAMTA gene contains salt stress regulatory elements, including ABRE, G-box, MBS, and TGA ( Wang et al, 2021 ), and CAMTAs of different species have been reported to respond to a variety of biotic and abiotic stresses, including low temperature, hormones, high salt, and drought. Two genes, ZmCAMTA4 and ZmCAMTA6 , were highly expressed under stress treatment, and cis-element analysis revealed the involvement of CAMTA genes in the association between environmental stress and stress-related hormones, and the GhCAMTA gene family may also be involved in the phytohormone signaling pathway ( Liu, Hewei & Min, 2021 ; Pant et al, 2018 ).…”