Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Li, Lu; Xu, Xihui; Chen, Chen; Shen, Zhenguo

doi:10.3390/ijms17101622

Cited by 45 publications

(75 citation statements)

References 66 publications

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“…Oxalate oxidase activity has been identified mainly in monocot plant species including wheat, barley, and rice (Svedruzic et al, 2005). Interestingly, germin-like proteins (GLPs) in Arabidopsis, which are homologous to oxalate oxidase, showed neither oxalate oxidase activity nor a Mndependent activation (Membré et al, 2000;Li et al, 2016). Intriguingly, the Mn 2+ cation itself may act as an antioxidant molecule.…”

Section: Functions Of Manganese In Plantsmentioning

confidence: 99%

Manganese in Plants: From Acquisition to Subcellular Allocation

et al. 2020

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Manganese (Mn) is an important micronutrient for plant growth and development and sustains metabolic roles within different plant cell compartments. The metal is an essential cofactor for the oxygen-evolving complex (OEC) of the photosynthetic machinery, catalyzing the water-splitting reaction in photosystem II (PSII). Despite the importance of Mn for photosynthesis and other processes, the physiological relevance of Mn uptake and compartmentation in plants has been underrated. The subcellular Mn homeostasis to maintain compartmented Mn-dependent metabolic processes like glycosylation, ROS scavenging, and photosynthesis is mediated by a multitude of transport proteins from diverse gene families. However, Mn homeostasis may be disturbed under suboptimal or excessive Mn availability. Mn deficiency is a serious, widespread plant nutritional disorder in dry, well-aerated and calcareous soils, as well as in soils containing high amounts of organic matter, where bio-availability of Mn can decrease far below the level that is required for normal plant growth. By contrast, Mn toxicity occurs on poorly drained and acidic soils in which high amounts of Mn are rendered available. Consequently, plants have evolved mechanisms to tightly regulate Mn uptake, trafficking, and storage. This review provides a comprehensive overview, with a focus on recent advances, on the multiple functions of transporters involved in Mn homeostasis, as well as their regulatory mechanisms in the plant's response to different conditions of Mn availability.

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Section: Functions Of Manganese In Plantsmentioning

confidence: 99%

Manganese in Plants: From Acquisition to Subcellular Allocation

et al. 2020

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“…From the plant host perspective, chitinases and GLPs were among the top 20 proteins identified by PLS-DA as the best at differentiating infected from mock-inoculated samples. An increased abundance of germin-like proteins (GLPs) in WB could be contributing to increased basal defense mechanisms, which are conserved in many plants and involve enzymatic activities like superoxide dismutase (SOD), oxalate oxidase, and polyphenol oxidase (PPO) [ 42 ]. Our results show that the host responses detected in WB are conserved in other Xanthomonas pathosystems, in which chitinases and GLPs in tolerant rice seedlings are prominent features.…”

Section: Discussionmentioning

confidence: 99%

Proteome Analysis of Walnut Bacterial Blight Disease

Sagawa

Assis

Zaini

et al. 2020

IJMS

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The interaction between the plant host, walnut (Juglans regia; Jr), and a deadly pathogen (Xanthomonas arboricola pv. juglandis 417; Xaj) can lead to walnut bacterial blight (WB), which depletes walnut productivity by degrading the nut quality. Here, we dissect this pathosystem using tandem mass tag quantitative proteomics. Walnut hull tissues inoculated with Xaj were compared to mock-inoculated tissues, and 3972 proteins were identified, of which 3296 are from Jr and 676 from Xaj. Proteins with differential abundance include oxidoreductases, proteases, and enzymes involved in energy metabolism and amino acid interconversion pathways. Defense responses and plant hormone biosynthesis were also increased. Xaj proteins detected in infected tissues demonstrate its ability to adapt to the host microenvironment, limiting iron availability, coping with copper toxicity, and maintaining energy and intermediary metabolism. Secreted proteases and extracellular secretion apparatus such as type IV pilus for twitching motility and type III secretion effectors indicate putative factors recognized by the host. Taken together, these results suggest intense degradation processes, oxidative stress, and general arrest of the biosynthetic metabolism in infected nuts. Our results provide insights into molecular mechanisms and highlight potential molecular tools for early detection and disease control strategies.

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“…For instance, the members of two blocks of GLPs on chromosome 4 are independently sorted in tandem (Table 1 ). Importantly, tandem duplications appear to play an important role in expansion of the GLP family in rice and Arabidopsis [ 57 ]. It is known that GLPs can function as a cofactor for reinforcement of the cell wall through the production of H 2 O 2 due to their SOD activity (e.g.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs

Cheng

Ming

et al. 2018

BMC Genomics

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BackgroundMetal tolerance is often an integrative result of metal uptake and distribution, which are fine-tuned by a network of signaling cascades and metal transporters. Thus, with the goal of advancing the molecular understanding of such metal homeostatic mechanisms, comparative RNAseq-based transcriptome analysis was conducted to dissect differentially expressed genes (DEGs) in maize roots exposed to cadmium (Cd) stress.ResultsTo unveil conserved Cd-responsive genes in cereal plants, the obtained 5166 maize DEGs were compared with 2567 Cd-regulated orthologs in rice roots, and this comparison generated 880 universal Cd-responsive orthologs groups composed of 1074 maize DEGs and 981 rice counterparts. More importantly, most of the orthologous DEGs showed coordinated expression pattern between Cd-treated maize and rice, and these include one large orthologs group of pleiotropic drug resistance (PDR)-type ABC transporters, two clusters of amino acid transporters, and 3 blocks of multidrug and toxic compound extrusion (MATE) efflux family transporters, and 3 clusters of heavy metal-associated domain (HMAD) isoprenylated plant proteins (HIPPs), as well as all 4 groups of zinc/iron regulated transporter protein (ZIPs). Additionally, several blocks of tandem maize paralogs, such as germin-like proteins (GLPs), phenylalanine ammonia-lyases (PALs) and several enzymes involved in JA biosynthesis, displayed consistent co-expression pattern under Cd stress.Out of the 1074 maize DEGs, approximately 30 maize Cd-responsive genes such as ZmHIPP27, stress-responsive NAC transcription factor (ZmSNAC1) and 9-cis-epoxycarotenoid dioxygenase (NCED, vp14) were also common stress-responsive genes reported to be uniformly regulated by multiple abiotic stresses. Moreover, the aforementioned three promising Cd-upregulated genes with rice counterparts were identified to be novel Cd-responsive genes in maize.Meanwhile, one maize glutamate decarboxylase (ZmGAD1) with Cd co-modulated rice ortholog was selected for further analysis of Cd tolerance via heterologous expression, and the results suggest that ZmGAD1 can confer Cd tolerance in yeast and tobacco leaves.ConclusionsThese novel findings revealed the conserved function of Cd-responsive orthologs and paralogs, which would be valuable for elucidating the genetic basis of the plant response to Cd stress and unraveling Cd tolerance genes.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5109-8) contains supplementary material, which is available to authorized users.

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Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Cited by 45 publications

References 66 publications

Manganese in Plants: From Acquisition to Subcellular Allocation

Manganese in Plants: From Acquisition to Subcellular Allocation

Proteome Analysis of Walnut Bacterial Blight Disease

Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs

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