Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs

Cheng, Dan; Ming, Tan; Yu, Haijuan; Li, Liang; Zhu, Dandan; Chen, Yahua; Jiang, Mingyi

doi:10.1186/s12864-018-5109-8

Cited by 34 publications

(16 citation statements)

References 94 publications

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“…The WRKY transcription factor family is one of the most important regulator families reported to be involved in a variety of heavy metal stresses. For example, several studies have revealed the differential transcript expression of WRKY transcription factors under Cd stress in different plant species (Yang et al, 2016;Hong et al, 2017;Cheng et al, 2018;Han et al, 2019;Sheng et al, 2019). An increasing number of WRKYs that were identified to confer Cd tolerance in plants also exemplify the importance of heavy metal tolerance in plants.…”

Section: Discussionmentioning

confidence: 99%

Transcription Factor GmWRKY142 Confers Cadmium Resistance by Up-Regulating the Cadmium Tolerance 1-Like Genes

et al. 2020

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Cadmium (Cd) is a widespread pollutant that is toxic to living organisms. Previous studies have identified certain WRKY transcription factors, which confer Cd tolerance in different plant species. In the present study, we have identified 29 Cd-responsive WRKY genes in Soybean [Glycine max (L.) Merr.], and confirmed that 26 of those GmWRKY genes were up-regulated, while 3 were down-regulated. We have also cloned the novel, positively regulated GmWRKY142 gene from soybean and investigated its regulatory mechanism in Cd tolerance. GmWRKY142 was highly expressed in the root, drastically up-regulated by Cd, localized in the nucleus, and displayed transcriptional activity. The overexpression of GmWRKY142 in Arabidopsis thaliana and soybean hairy roots significantly enhanced Cd tolerance and lead to extensive transcriptional reprogramming of stress-responsive genes. ATCDT1, GmCDT1-1, and GmCDT1-2 encoding cadmium tolerance 1 were induced in overexpression lines. Further analysis showed that GmWRKY142 activated the transcription of ATCDT1, GmCDT1-1, and GmCDT1-2 by directly binding to the W-box element in their promoters. In addition, the functions of GmCDT1-1 and GmCDT1-2, responsible for decreasing Cd uptake, were validated by heterologous expression in A. thaliana. Our combined results have determined GmWRKYs to be newly discovered participants in response to Cd stress, and have confirmed that GmWRKY142 directly targets ATCDT1, GmCDT1-1, and GmCDT1-2 to decrease Cd uptake and positively regulate Cd tolerance. The GmWRKY142-GmCDT1-1/2 cascade module provides a potential strategy to lower Cd accumulation in soybean.

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Section: Discussionmentioning

confidence: 99%

Transcription Factor GmWRKY142 Confers Cadmium Resistance by Up-Regulating the Cadmium Tolerance 1-Like Genes

et al. 2020

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“… 131 GAD genes are uniformly up-regulated in maize and rice roots by Cd stress, and the overexpression of ZmGAD1 and ZmGAD2 in Cd-sensitive yeast and tobacco leaves enhances Cd tolerance in the host cells. 132 All of the aforementioned studies indicate that GABA content does not always increase in response to different metal stresses and may be more related to metal concentrations. Furthermore, high GABA content does not always enhance plant tolerance to metal stress.…”

Section: Abiotic and Biotic Stressmentioning

confidence: 99%

The versatile GABA in plants

Dou

Zhang

et al. 2021

Plant Signaling & Behavior

175

100

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Gamma-aminobutyric acid (GABA) is a ubiquitous four-carbon, non-protein amino acid. GABA has been widely studied in animal central nervous systems, where it acts as an inhibitory neurotransmitter. In plants, it is metabolized through the GABA shunt pathway, a bypass of the tricarboxylic acid (TCA) cycle. Additionally, it can be synthesized through the polyamine metabolic pathway. GABA acts as a signal in Agrobacterium tumefaciens -mediated plant gene transformation and in plant development, especially in pollen tube elongation (to enter the ovule), root growth, fruit ripening, and seed germination. It is accumulated during plant responses to environmental stresses and pathogen and insect attacks. A high concentration of GABA elevates plant stress tolerance by improving photosynthesis, inhibiting reactive oxygen species (ROS) generation, activating antioxidant enzymes, and regulating stomatal opening in drought stress. The transporters of GABA in plants are reviewed in this work. We summarize the recent research on GABA function and transporters with the goal of providing a review of GABA in plants.

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“…Previous study conducted in maize and rice plants have revealed that cd-responsive orthologous are conserved in both plants. Among which ZmGAD1 gene, responsible for glutamate decarboxylase (GAD) enzyme (a key enzyme in GABA biosynthesis) has been introduced as a novel Cd-responsive gene and successfully conferred with Cd tolerance in yeast 75 . Here we have focused on GABA modulatory effect on PAs as different line of the tolerance pathway in maize plants.…”

mentioning

confidence: 99%

γ-Aminobutyric acid confers cadmium tolerance in maize plants by concerted regulation of polyamine metabolism and antioxidant defense systems

Seifikalhor

Aliniaeifard

Bernard

et al. 2020

Sci Rep

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Gamma-Aminobutyric acid (GABA) accumulates in plants following exposure to heavy metals. to investigate the role of GABA in cadmium (cd) tolerance and elucidate the underlying mechanisms, GABA (0, 25 and 50 µM) was applied to cd-treated maize plants. Vegetative growth parameters were improved in both cd-treated and control plants due to GABA application. cd uptake and translocation were considerably inhibited by GABA. Antioxidant enzyme activity was enhanced in plants subjected to cd. concurrently GABA caused further increases in catalase and superoxide dismutase activities, which led to a significant reduction in hydrogen peroxide, superoxide anion and malondealdehyde contents under stress conditions. polyamine biosynthesis-responsive genes, namely ornithine decarboxylase and spermidine synthase, were induced by GABA in plants grown under cd shock. GABA suppressed polyamine oxidase, a gene related to polyamine catabolism, when plants were exposed to Cd. Consequently, different forms of polyamines were elevated in Cd-exposed plants following GABA application. The maximum quantum efficiency of photosystem II (F v /f m) was decreased by cd-exposed plants, but was completely restored by GABA to the same value in the control. these results suggest a multifaceted contribution of GABA, through regulation of cd uptake, production of reactive oxygen species and polyamine metabolism, in response to cd stress. Heavy metal (HM) toxicity is considered a major threat to living organisms. HM-polluted soils derived from increasing geologic and anthropogenic activities have significantly impacted the production of high-quality agricultural crops in certain regions of the world. Plants growing on these soils exhibit reduced growth, photosynthetic performance, and yield 1. As a naturally occurring HM pollutant, Cd exposure has been documented in most organisms, particularly plants and humans 2. World fertilizer consumption is increasing and will eventually reach a point where the drawbacks outweigh the benefits 3. The same mechanisms that drive improved plant productivity often create side effects such as environmental contamination. Furthermore, some components of fertilizers, especially Cd, accumulate in both body and food chain, where they remain for an extended period and causes adverse health effects 4. Therefore, fertilizers containing very high levels of Cd (417 mg/kg) threaten human health by accumulation in important crop such as maize 5. Maize, as one of the most popular cereal grain, is widely cultivated across the world. Maize is also produced at an industrial scale as a key input in various products such as syrups, soft drinks and charcoals 6,7. Therefore, there is a strong incentive to minimize the toxic effects of Cd in maize.

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Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs

Cited by 34 publications

References 94 publications

Transcription Factor GmWRKY142 Confers Cadmium Resistance by Up-Regulating the Cadmium Tolerance 1-Like Genes

Transcription Factor GmWRKY142 Confers Cadmium Resistance by Up-Regulating the Cadmium Tolerance 1-Like Genes

The versatile GABA in plants

γ-Aminobutyric acid confers cadmium tolerance in maize plants by concerted regulation of polyamine metabolism and antioxidant defense systems

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