Genome-Wide Analysis of LncRNA in Bovine Mammary Epithelial Cell Injuries Induced by Escherichia Coli and Staphylococcus Aureus

Abstract

Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to investigate the effects of bovine mammary epithelial cell injuries induced by treatment with E. coli and S. aureus, and to explore the lncRNA profile on cell injuries. The lncRNA transcriptome analysis showed a total of 2597 lncRNAs. There were 2234 lncRNAs differentially expressed in the E. coli group and 2334 in the S. aureus group. Moreover, we found that the E. coli and S. aureus groups of maternal genes targeted signaling pathways with similar functions according to KEGG and GO analyses. Two lncRNA–miRNA–mRNA interaction networks were constructed in order to predict the potential molecular mechanisms of regulation in the cell injuries. We believe that this is the first report demonstrating the dysregulation of lncRNAs in cells upon E. coli and S. aureus infections, suggesting that they have the potential to become important diagnostic markers and to provide novel insights into controlling and preventing mastitis.

Characterization of peripheral white blood cells transcriptome to unravel the regulatory signatures of bovine subclinical mastitis resistance

“…In this study, the expression profiles of mRNAs and lncRNAs from healthy cows and subclinical mastitis cows were characterized, and the functions of 287 DEGs, 70 DELs, and 1,621 DAS genes were annotated, obtaining a number of significantly enriched pathways. Notably, the NOD-like receptor signaling pathway, cytokine-cell receptor interaction, Toll-like receptor signaling pathway, and S. aureus infection pathway were also significantly enriched in the available studies of bovine mammary epithelial (BME) cells ( Wang et al, 2016 ; Wang et al, 2020a ; Lin et al, 2021 ), suggesting that these pathways play important regulatory roles in the pathogenesis of mastitis in blood and breast tissue. Subsequently, based on target gene prediction and QTL database annotation, the results showed that the lncRNAs MSTRG25101.2, MSTRG.56327 0.1, and MSTRG.18968.1, which are adjacent to the SCS QTL and SCC QTL, regulate the expression levels of genes NOD2 , CXCL8 , OAS2, and TLR4 , which are involved in the NOD-like receptor signaling pathway, Cytokine-cytokine receptor interaction, and Toll-like receptor signaling pathway.…”

N6-Methyladenosine-Modified circRNA in the Bovine Mammary Epithelial Cells Injured by Staphylococcus aureus and Escherichia coli

“…This response occurs through an inflammatory-related pathway ( 10 – 13 ). Some studies have confirmed the immune response of mastitis in S. aureus and E. coli infection ( 14 – 18 ). However, the regulatory function of noncoding RNA and epigenetic modification is ignored.…”

Role of Long Noncoding RNAs in the Regulation of Cellular Immune Response and Inflammatory Diseases

“…LncRNAs regulate inflammatory factor expression and inflammatory signaling pathways by interacting with specific miRNAs, mRNAs, and proteins at the transcriptional and post-transcriptional levels, ultimately alleviating inflammatory damage. At present, some progress has been made regarding research on lncRNAs in the diagnosis and treatment of inflammatory diseases such as acute kidney injury (AKI) [ 64 ], liver inflammation [ 65 ], ALI [ 66 ], OA [ 67 ], mastitis [ 68 ], and neuroinflammation [ 69 ] ( Table 2 ). This section primarily focuses on the roles of lncRNAs in inflammatory diseases, to provide some reference for further research on lncRNAs in inflammatory diseases.…”