2019
DOI: 10.23855/preslia.2019.117
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Abstract: Genome sizes and genomic guanine+cytosine (GC) contents of the Czech vascular flora with new estimates for 1700 species.-Preslia 91: 117-142. The content of DNA in the somatic, unreplicated cell nuclei (genome size) and DNA base composition (GC content) are the basic genomic parameters that can be measured by flow cytometry. Genome size, or ploidy level, can affect many plant properties and are therefore included as important features in modern biological floras and plant trait databases. However, genomic data… Show more

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Cited by 32 publications
(36 citation statements)
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References 87 publications
(121 reference statements)
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“…2C/ploidy level; Greilhuber et al ., 2005) were adopted from a flow cytometry survey by Šmarda et al . (2019). In species with multiple ploidy levels, variable genome size, and/or complex taxonomy, genome sizes were measured using flow cytometry directly in plants on which the flowering dates were recorded – using flow cytometry with propidium iodide dye following the protocol in Šmarda et al .…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…2C/ploidy level; Greilhuber et al ., 2005) were adopted from a flow cytometry survey by Šmarda et al . (2019). In species with multiple ploidy levels, variable genome size, and/or complex taxonomy, genome sizes were measured using flow cytometry directly in plants on which the flowering dates were recorded – using flow cytometry with propidium iodide dye following the protocol in Šmarda et al .…”
Section: Methodsmentioning
confidence: 99%
“…In species with multiple ploidy levels, variable genome size, and/or complex taxonomy, genome sizes were measured using flow cytometry directly in plants on which the flowering dates were recorded – using flow cytometry with propidium iodide dye following the protocol in Šmarda et al . (2019). Genome size was measured in one individual per species.…”
Section: Methodsmentioning
confidence: 99%
“…However, this approach is for many non-model species like comfrey, still not straightforward due to non-existent molecular markers. Therefore, we first developed markers based on next-generation sequencing (NGS) approach and then determined the relationships of different accessions by single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP), a fluorescence-based genotyping technology that evolved into a global benchmark technology (Rasheed et al 2017;Semagn et al 2014).…”
Section: Introductionmentioning
confidence: 99%
“…Currently, the largest updated plant genome size database—Plant DNA C-values database contains data for 12,273 species and among them 65 C-values for 44 species of genus Iris [ 26 ]. For most species involved in our study C-values are measured in several studies [ 27 , 28 , 29 ]. Different methods were used for the measurement of plant DNA content, but flow cytometry has become the method of choice due to its reliability, simplicity, and relatively low cost [ 30 , 31 ].…”
Section: Introductionmentioning
confidence: 99%