Genome Sequence of the Trichosporon asahii Environmental Strain CBS 8904

Yang, Rong; Li, Hai Tao; Zhu, He; Zhou, Guangpeng; Wang, Meng; Wang, Lei

doi:10.1128/ec.00264-12

Cited by 17 publications

(10 citation statements)

References 15 publications

(15 reference statements)

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“…Searches with HD proteins from other Tremellomycetes in the predicted T. oleaginosus proteins as well as tBLASTn searches in the genome assembly did not yield any putative Sxi2 homologs either. This was further confirmed by searches in the genomes of the T. asahii type strain ( 26 ) (see Table S2 ) and a T. asahii environmental strain ( 25 ) (data not shown), indicating that the currently available Trichosporon genomes do not encode an HD2 transcription factor. Homologs for the pheromone genes were also not found; however, this is not unusual, as pheromones are small peptides that harbor only short conserved motifs.…”

Section: Resultsmentioning

confidence: 71%

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus : Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Kourist

Bracharz

Lorenzen

et al. 2015

mBio

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Microbial fermentation of agro-industrial waste holds great potential for reducing the environmental impact associated with the production of lipids for industrial purposes from plant biomass. However, the chemical complexity of many residues currently prevents efficient conversion into lipids, creating a high demand for strains with the ability to utilize all energy-rich components of agricultural residues. Here, we present results of genome and transcriptome analyses of Trichosporon oleaginosus. This oil-accumulating yeast is able to grow on a wide variety of substrates, including pentoses and N-acetylglucosamine, making it an interesting candidate for biotechnological applications. Transcriptomics shows specific changes in gene expression patterns under lipid-accumulating conditions. Furthermore, gene content and expression analyses indicate that T. oleaginosus is well-adapted for the utilization of chitin-rich biomass. We also focused on the T. oleaginosus mating type, because this species is a member of the Tremellomycetes, a group that has been intensively analyzed as a model for the evolution of sexual development, the best-studied member being Cryptococcus neoformans. The structure of the T. oleaginosus mating-type regions differs significantly from that of other Tremellomycetes and reveals a new evolutionary trajectory paradigm. Comparative analysis shows that recruitment of developmental genes to the ancestral tetrapolar mating-type loci occurred independently in the Trichosporon and Cryptococcus lineages, supporting the hypothesis of a trend toward larger mating-type regions in fungi.

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Section: Resultsmentioning

confidence: 71%

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus : Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Kourist

Bracharz

Lorenzen

et al. 2015

mBio

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“…globosa , and M . sympodialis , which range from 7.6 to 9.0 Mbp and are predicted to encode around 3,500 to 4,300 genes [ 24 , 25 ]. In contrast, the genomes of Cryptococcus neoformans and C .…”

Section: What Are Trichosporon Species?mentioning

confidence: 99%

On and Under the Skin: Emerging Basidiomycetous Yeast Infections Caused by Trichosporon Species

et al. 2015

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“…Other haplotypes are described in Spain, Japan and Brazil 20,21 . In 2012, Yang et al sequenced the whole genome of two strains of T. asahii (an environmental isolate, CBS8904, recovered from maize cobs, in 1998, Illinois, USA 22 and a clinical isolate, CBS2479, the type strain, recovered in Japan in 1929 from a psoriasis lesion) 23 …”

Section: Introductionmentioning

confidence: 99%

Epidemiological investigation for grouped cases of Trichosporon asahii using whole genome and IGS1 sequencing

Desnos‐Ollivier

Maufrais

Pihet

et al. 2020

Mycoses

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Summary Background Trichosporonosis is a rare invasive infection in humans mainly due to Trichosporon asahii, and especially recovered from patients having haematological malignancy. Since 2012, IGS1 region sequencing is used as a genotyping method to distinguish isolates, with high frequency of one haplotype worldwide and a geographic specificity for some haplotypes. Objectives We compared the IGS1 genotyping method and whole genome sequencing (WGS) to study the relationship between clinical isolates involved in two grouped cases in France. Methods IGS1 sequencing and antifungal susceptibility testing were performed for 54 clinical isolates. Clinical data for 28 isolates included in surveillance programs were analysed. Whole genome was sequenced for 32 clinical isolates and the type strain. Results All isolates were intrinsically resistant to flucytosine, while voriconazole had the most potent in vitro activity. The majority of the isolates was recovered from patients with haematological malignancies (42.86%), with a high proportion of children (<15 yrs‐old, 32.14%) and a high mortality rate at three months (46.15%). Based on the WGS analysis, isolates exhibiting IGS1 haplotype 1, 3 and 7 belonged to different clades. Five isolates recovered during the first grouped cases had the same IGS1 haplotype and shared 99% of SNPs similarity. For the second grouped cases, four isolates had 98.7% of SNPs similarity while the isolate recovered 4 years earlier was totally unlinked. Conclusions We confirmed the usefulness of IGS1 sequencing for grouped cases infection of T. asahii. We underlined its limitation for the study of population structure and the utility of WGS analysis for the study of epidemiologically unrelated isolates.

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Genome Sequence of the Trichosporon asahii Environmental Strain CBS 8904

Cited by 17 publications

References 15 publications

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus : Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus : Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

On and Under the Skin: Emerging Basidiomycetous Yeast Infections Caused by Trichosporon Species

Epidemiological investigation for grouped cases of Trichosporon asahii using whole genome and IGS1 sequencing

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