Genome Sequence of the Extreme Obligate Alkaliphile Bacillus marmarensis Strain DSM 21297

Wernick, David G.; Choi, Kwon‐Young; Tat, Christine A.; Rivera, Jimmy G. Lafontaine; Liao, James C.

doi:10.1128/genomea.00967-13

Cited by 7 publications

(9 citation statements)

References 15 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, no strains able to grow in a higher pH medium have been identified. Previously, we sequenced and annotated its genome 16 . We began further study by characterizing its growth rate across a wide pH range ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…B. marmarensis was previously shown to utilize cellobiose, but not xylose 15 . However, we predicted otherwise as we identified xylose isomerase and xylulose kinase genes within its genome 16 that may allow it to assimilate xylose into the pentose phosphate pathway and central metabolism. During fermentations, B. marmarensis achieved yields of 53 and 58% the theoretical maximum from cellobiose and xylose, respectively, and 53% yield at a titer of 12.3 ± 1.2 g/l from a 50:50 mass mixture of both ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…B. pseudofirmus OF4, B. clausii KSM-K16, and B. halodurans C-125 were obtained from ATCC (Manassas, VA – ATCC BAA-2126, 31084 and BAA-125, respectively). Escherichia coli strain JCL16 was used as described elsewhere 16 . B. subtilis 168 was obtained from BGSC (Columbus, OH, Strain 1A1).…”

Section: Methodsmentioning

confidence: 99%

“…Ethanol was quantified by gas chromatography with flame ionization detection as previously described 16 44 . Lactate, succinate, acetate, glucose, cellobiose, and xylose were identified and quantified by HPLC analysis on an Aminex HPX-87H column (Bio-Rad) as described previously, but changing the mobile phase from 5 mM H 2 SO 4 to 30 mM H 2 SO 4 44 .…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis

Wernick

Pontrelli

Pollock

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Contamination susceptibility, water usage, and inability to utilize 5-carbon sugars and disaccharides are among the major obstacles in industrialization of sustainable biorefining. Extremophilic thermophiles and acidophiles are being researched to combat these problems, but organisms which answer all the above problems have yet to emerge. Here, we present engineering of the unexplored, extreme alkaliphile Bacillus marmarensis as a platform for new bioprocesses which meet all these challenges. With a newly developed transformation protocol and genetic tools, along with optimized RBSs and antisense RNA, we engineered B. marmarensis to produce ethanol at titers of 38 g/l and 65% yields from glucose in unsterilized media. Furthermore, ethanol titers and yields of 12 g/l and 50%, respectively, were produced from cellobiose and xylose in unsterilized seawater and algal-contaminated wastewater. As such, B. marmarensis presents a promising approach for the contamination-resistant biorefining of a wide range of carbohydrates in unsterilized, non-potable seawater.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis

Wernick

Pontrelli

Pollock

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, Bacillus marmarensis , an extreme obligate alkaliphilic and protease-producing bacterium, has been reported as a sustainable microorganism for biorefining, allowing sterilization to be eliminated without the risk of contamination. Additionally, the metabolic pathway for poly(3-hydroxybutyrate) was annotated [ 14 , 15 , 16 ]. Within the frame of references, the production of PHB from B. marmarensis was evaluated for the production of poly(3-hydroxybutyrate), and the effect of environmental conditions and medium components on PHB synthesis was investigated [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72T Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis

2021

View full text Add to dashboard Cite

The novel extreme obligate alkaliphilic Bacillus marmarensis DSM 21297 is known to produce polyhydroxybutyrate (PHB). However, the detailed mechanism of PHB synthesis in B. marmarensis is still unknown. Here, we investigated which metabolic pathways and metabolic enzymes are responsible for PHB synthesis in order to understand the regulatory pathway and optimize PHB synthesis in B. marmarensis. In accordance with the fact that beta-galactosidase, 3-hydroxyacyl-CoA dehydrogenase, and Enoyl-CoA hydratase together with acyl-CoA dehydrogenase and lipase were annotated in B. marmarensis according to the RAST server, we used glucose, lactose, and olive oil to understand the preferred metabolic pathway for the PHB synthesis. It was found that B. marmarensis produces PHB from glucose, lactose, and olive oil. However, the highest PHB titer and the highest amount of PHB synthesized per dry cell mass (YP/X) were achieved in the presence of lactose, as compared to glucose and olive oil. Additionally, in the absence of peptone, the amount of PHB synthesized is reduced for each carbon source. Interestingly, none of the carbon sources studied yielded an efficient PHB synthesis, and supplementation of the medium with potassium ions did not enhance PHB synthesis. According to these experimental results and the presence of annotated metabolic enzymes based on the RAST server, PHB accumulation in the cells of B. marmarensis could be improved by the level of the expression of 3-hydroxybutyryl-CoA dehydrogenase (1.1.1.157), which increases the production of NADPH. Additionally, the accumulation of 3-hydroxyacyl-CoA could enhance the production of PHB in B. marmarensis in the presence of fatty acids. To our knowledge, this is the first report investigating the regulatory system involved in the control of PHB metabolism of B. marmarensis.

show abstract

Alkaliphiles: The Versatile Tools in Biotechnology

Mamo¹,

Mattìasson

2020

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

Genome Sequence of the Extreme Obligate Alkaliphile Bacillus marmarensis Strain DSM 21297

Cited by 7 publications

References 15 publications

Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis

Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis

Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72T Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis

Alkaliphiles: The Versatile Tools in Biotechnology

Contact Info

Product

Resources

About