Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis

Wernick, David G.; Pontrelli, Sammy; Pollock, Alexander W.; Liao, James C.

doi:10.1038/srep20224

Cited by 34 publications

(25 citation statements)

References 42 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…E. coli is incapable of producing glycosylated biopharmaceutical products, proteins that require complex assembly, or proteins with high numbers of disulfide bonds (Meyer and Schmidhalter, 2012). In addition, E. coli is not suitable for culturing conditions at high (Tao et al, 2005) and low pH (Wernick et al, 2016), and high temperatures (Hasunuma and Kondo, 2012;Bhalla et al, 2013). These conditions present advantages for contamination resistance (Tao et al, 2005;Wernick et al, 2016), reduced titration requirements, and consolidated bioprocesses (Hasunuma and Kondo, 2012;Bhalla et al, 2013;Olson et al, 2012) in which certain substrates can be broken down and consumed simultaneously.…”

Section: Why E Coli?mentioning

confidence: 99%

Escherichia coli as a host for metabolic engineering

Pontrelli

Chiu

Lan

et al. 2018

Metabolic Engineering

Self Cite

279

140

View full text Add to dashboard Cite

Over the past century, Escherichia coli has become one of the best studied organisms on earth. Features such as genetic tractability, favorable growth conditions, well characterized biochemistry and physiology, and availability of versatile genetic manipulation tools make E. coli an ideal platform host for development of industrially viable productions. In this review, we discuss the physiological attributes of E. coli that are most relevant for metabolic engineering, as well as emerging techniques that enable efficient phenotype construction. Further, we summarize the large number of native and non-native products that have been synthesized by E. coli, and address some of the future challenges in broadening substrate range and fighting phage infection.

show abstract

Section: Why E Coli?mentioning

confidence: 99%

Escherichia coli as a host for metabolic engineering

Pontrelli

Chiu

Lan

et al. 2018

Metabolic Engineering

Self Cite

279

140

View full text Add to dashboard Cite

show abstract

“…Previously, 15 g/L of 1-butanol was produced within engineered E.coli by deletion of fermentative pathways, thereby requiring the use of the 1-butanol pathway as the sole NADH sink under anaerobic conditions (Shen et al, 2011). While this strategy yielded 1-butanol that is regarded as one of the highest biofuel production to date, Friedlander et al, 2016;Inokuma et al, 2010;Pais et al, 2013;Wernick et al, 2016;York and Ingram, 1996) improved titers are desired. In addition to the deletion of NADH consuming pathways, many other metabolic engineering strategies have been applied in order to achieve such high titers.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics-driven approach to solving a CoA imbalance for improved 1-butanol production in Escherichia coli

Ohtake

Pontrelli

Laviña

et al. 2017

Metabolic Engineering

Self Cite

View full text Add to dashboard Cite

A B S T R A C THigh titer 1-butanol production in Escherichia coli has previously been achieved by overexpression of a modified clostridial 1-butanol production pathway and subsequent deletion of native fermentation pathways. This strategy couples growth with production as 1-butanol pathway offers the only available terminal electron acceptors required for growth in anaerobic conditions. With further inclusion of other well-established metabolic engineering principles, a titer of 15 g/L has been obtained. In achieving this titer, many currently existing strategies have been exhausted, and 1-butanol toxicity level has been surpassed. Therefore, continued engineering of the host strain for increased production requires implementation of alternative strategies that seek to identify non-obvious targets for improvement. In this study, a metabolomics-driven approach was used to reveal a CoA imbalance resulting from a pta deletion that caused undesirable accumulation of pyruvate, butanoate, and other CoA-derived compounds. Using metabolomics, the reduction of butanoyl-CoA to butanal catalyzed by alcohol dehydrogenase AdhE2 was determined as a rate-limiting step. Fine-tuning of this activity and subsequent release of free CoA restored the CoA balance that resulted in a titer of 18.3 g/L upon improvement of total free CoA levels using cysteine supplementation. By enhancing AdhE2 activity, carbon flux was directed towards 1-butanol production and undesirable accumulation of pyruvate and butanoate was diminished. This study represents the initial report describing the improvement of 1-butanol production in E. coli by resolving CoA imbalance, which was based on metabolome analysis and rational metabolic engineering strategies.

show abstract

“…Here we’ve described three novel phages isolated from halo-alkaline environments, and as very few phages that infect alkaliphic and more specifically haloalkaliphilic microorganisms have been described, these three phages are rather unique [46, 66, 67]. It is unknown exactly what the role of the host organisms identified here is in their respective environments, however the presence of possibly lytic phages such as Shpa and Shbh1, could have a significant impact on their hosts and therefore the environment in which they find themselves.…”

Section: Resultsmentioning

confidence: 99%

Three novel bacteriophages isolated from the East African Rift Valley soda lakes

Zyl

Nemavhulani

Cass

et al. 2016

Virol J

View full text Add to dashboard Cite

BackgroundSoda lakes are unique environments in terms of their physical characteristics and the biology they harbour. Although well studied with respect to their microbial composition, their viral compositions have not, and consequently few bacteriophages that infect bacteria from haloalkaline environments have been described.MethodsBacteria were isolated from sediment samples of lakes Magadi and Shala. Three phages were isolated on two different Bacillus species and one Paracoccus species using agar overlays. The growth characteristics of each phage in its host was investigated and the genome sequences determined and analysed by comparison with known phages.ResultsPhage Shbh1 belongs to the family Myoviridae while Mgbh1 and Shpa belong to the Siphoviridae family. Tetranucleotide usage frequencies and G + C content suggests that Shbh1 and Mgbh1 do not regularly infect, and have therefore not evolved with, the hosts they were isolated on here. Shbh1 was shown capable of infecting two different Bacillus species from the two different lakes demonstrating its potential broad-host range. Comparative analysis of their genome sequence with known phages revealed that, although novel, Shbh1 does share substantial amino acid similarity with previously described Bacillus infecting phages (Grass, phiNIT1 and phiAGATE) and belongs to the Bastille group, while Mgbh1 and Shpa are highly novel.ConclusionThe addition of these phages to current databases should help with metagenome/metavirome annotation efforts. We describe a highly novel Paracoccus infecting virus (Shpa) which together with NgoΦ6 and vB_PmaS_IMEP1 is one of only three phages known to infect Paracoccus species but does not show similarity to these phages.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0656-6) contains supplementary material, which is available to authorized users.

show abstract

Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis

Cited by 34 publications

References 42 publications

Escherichia coli as a host for metabolic engineering

Escherichia coli as a host for metabolic engineering

Metabolomics-driven approach to solving a CoA imbalance for improved 1-butanol production in Escherichia coli

Three novel bacteriophages isolated from the East African Rift Valley soda lakes

Contact Info

Product

Resources

About