Genetic variation within and between G1 and G3 genotypes of Echinococcus granulosus in Italy revealed by multilocus DNA sequencing

Busi, Marina; Šnábel, Viliam; Varcasia, Antonio; Garippa, Giovanni; Perrone, Vittorio; Liberato, Claudio De; D’Amelio, Stefano

doi:10.1016/j.vetpar.2007.09.003

Cited by 100 publications

(79 citation statements)

References 30 publications

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“…This data is consistent with results reported by other surveys in Italy, where cattle seems to be mostly infected by sheep strain (G1). The G5 specific cattle strain or E. ortleppi was found only once in one cattle imported from Switzerland to Northern Italy [15,17,18,40].…”

Section: Discussionmentioning

confidence: 99%

Retrospective study on Cystic Echinococcosis in cattle of Italy

Poglayen

Varcasia

Pipia

et al. 2017

J Infect Dev Ctries

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Introduction: Cystic Echinococcosis (CE) is one of the most widespread zoonosis of veterinary and medical importance still constituting a sanitary, economic and socio-cultural problem in Italy. Methodology: The aim of this study was to update epidemiological data on cattle CE in Italy. Data on CE positivity of 5,336 cattle were acquired from abattoir registers between January 2009 and July 2010. Morphobiological characterization of hydatids was performed by direct examination of liver and lungs of 1,664 animals butchered in the same slaughterhouses in 2010. Strain typing of parasites was carried out through the amplification and sequencing of nd1 and cox1 mitochondrial genes. Results: Overall CE prevalence was of 8.1% (430/5,336). Parasitological examination of hydatids showed an overall prevalence of 8.6% with a fertility rate of 0.7% (12/1,664). Regarding localization, hydatids were found in 8% of the livers and in 7.6% of the lungs, respectively. Among positive animals, higher prevalence was observed in the liver (93%) compared to lungs (88.1%) (p > 0.05). Conclusion: The economic loss due to organs condemnation related to CE in cattle amounted to almost € 24,000 per year in the examined abattoir during 2010. Sequence analysis showed the presence of G1 (sheep strain) or Echinococcus granulosus sensu strictu in all examined samples. The G1 confirmed, once more, its possible development into several intermediate hosts such as cattle, especially in areas like southern Italy and Sardinia where the lifecycle of the parasite is still to date carried on by sheep and dogs.

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Section: Discussionmentioning

confidence: 99%

Retrospective study on Cystic Echinococcosis in cattle of Italy

Poglayen

Varcasia

Pipia

et al. 2017

J Infect Dev Ctries

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“…[48][49][50][51][52][53][54][55] For the first time in Iran, Sharbatkhori and others 30 reported the occurrence of the G3 genotype in camels from the Isfahan Province (central Iran). This was a global first for the identification of the G3 genotype in this host.…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Echinococcus granulosus from a Large Number of Formalin-Fixed, Paraffin-Embedded Tissue Samples of Human Isolates in Iran

Rostami¹,

Torbaghan²,

Dabiri³

et al. 2015

The American Journal of Tropical Medicine and Hygiene

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Abstract. Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus, presents an important medical and veterinary problem globally, including that in Iran. Different genotypes of E. granulosus have been reported from human isolates worldwide. This study identifies the genotype of the parasite responsible for human hydatidosis in three provinces of Iran using formalin-fixed paraffin-embedded tissue samples. In this study, 200 formalin-fixed paraffin-embedded tissue samples from human CE cases were collected from Alborz, Tehran, and Kerman provinces. Polymerase chain reaction amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 gene were performed for genetic characterization of the samples. Phylogenetic analysis of the isolates from this study and reference sequences of different genotypes was done using a maximum likelihood method. In total, 54.4%, 0.8%, 1%, and 40.8% of the samples were identified as the G1, G2, G3, and G6 genotypes, respectively. The findings of the current study confirm the G1 genotype (sheep strain) to be the most prevalent genotype involved in human CE cases in Iran and indicates the high prevalence of the G6 genotype with a high infectivity for humans. Furthermore, this study illustrates the first documented human CE case in Iran infected with the G2 genotype.

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“…Echinococcus granulosus (G1eG3) is the species most frequently identified in domestic animals and humans in southern Europe Busi et al, 2007;. Although the typical life cycle patterns involve livestock and domestic dogs, E. granulosus is also known to occur in wolves in Italy and Spain Gori et al, 2015) and in wild boars in Spain, Italy and Greece Mwambete et al, 2004;, highlighting that the wild animal cycle must be also taken into account in the epidemiology of E. granulosus.…”

Section: U N C O R R E C T E D P R O O F S0360 3442 Molecular Epidmentioning

confidence: 99%