Genetic variation of Coilia ectenes (Clupeiformes: Engraulidae) revealed by the complete cytochrome b sequences of mitochondrial DNA

Ma, Chunyan; Cheng, Qiqun; Zhang, Qingyi; Zhuang, Ping; Zhao, Yunlong

doi:10.1016/j.jembe.2010.01.015

Cited by 72 publications

(47 citation statements)

References 26 publications

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“…Cytochrome b gene is among mtD-NA genes which is familiar in population analysis. Previous studies, either using PCR-RFLP or sequences of cytochorme b gene have proven that this gene is variable enough to discriminate among populations on various fish species (Mesquita et al, 2001;Takehana et al, 2004;Cheng & Lu, 2005;Ma et al, 2010) and from pork in meatballs product (Fibriana et al, 2012). However, those studies were done to evaluate genetic differentiation among populations.…”

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confidence: 99%

Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

et al. 2017

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Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome bAluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b-HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.

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confidence: 99%

Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

et al. 2017

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“…Genomic DNAs were extracted from the muscle tissue using a standard phenolchloroform extraction protocol (Ma et al, 2010a).…”

Section: Sample Collection and Dna Extractionmentioning

confidence: 99%

Molecular identification of the genus Thryssa based on DNA barcoding

Cy¹,

Hy²,

Ni³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. DNA barcoding is an effective method for identifying species by analyzing one or a few short standardized DNA sequences. In this study, we examined the utility of mitochondrial cytochrome oxidase subunit I (COI) sequences as a DNA barcode for the identification of six species belonging to the genus Thryssa: T. dussumieri, T. hamiltonii, T. kammalensis, T. mystax, T. setirostris, and T. vitrirostris. We obtained an intraspecific distance of 0.000 for T. vitrirostris and T. hamiltonii, 0.006 for T. mystax, 0.002 for T. dussumieri, and 0.005 for T. kammalensis. The average intraspecific distance was 0.002, while the average interspecific distance was 0.137. Thus, the interspecific genetic distance was approximately 67-fold larger than the intraspecific genetic distance; the average genetic distance among species was greater than the minimum of 0.020 between species suggested elsewhere. The genetic distance between T. vitrirostris and T. mystax was 0.003. A maximum-likelihood phylogenetic tree constructed using bestfitting tree topology showed distinct clusters corresponding to the species (except for T. vitrirostris and T. mystax). The closest relationship was found between T. vitrirostris and T. mystax. These two species clustered together in the phylogenetic tree. This conclusion contradicts the evolutionary 18581 Molecular identification of the genus Thryssa ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18580-18586 (2015) relationship based on morphological classification.

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“…Genomic DNA was isolated from muscle tissue using a traditional phenol-chloroform extraction protocol, as described by Ma et al (2010a).…”

Section: Sample Collection and Dna Extractionmentioning

confidence: 99%

Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin

Yx¹,

Cy²,

Hy³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Nine microsatellite DNA markers were developed and characterized for Siganus oramin by the 5'-anchored polymerase chain reaction technique. A total of 42 alleles were identified in 30 individuals, and the number of alleles per locus ranged from 3 to 7, with an average of 4.7. The observed and expected heterozygosity per locus ranged from 0.5333 to 1.0000 and from 0.5254 to 0.8474, respectively, with an average of 0.7422 and 0.6906, respectively. A significant deviation from the HardyWeinberg equilibrium was detected at one microsatellite locus after a Bonferroni's correction (P < 0.0056). No significant linkage disequilibrium was found between any of the pairs of the nine loci. The microsatellite loci developed in this study will improve our understanding of the genetic background of S. oramin.

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Genetic variation of Coilia ectenes (Clupeiformes: Engraulidae) revealed by the complete cytochrome b sequences of mitochondrial DNA

Cited by 72 publications

References 26 publications

Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

Molecular identification of the genus Thryssa based on DNA barcoding

Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin

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