Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

Abstract

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome bAluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b-HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.

Genetic Homogenity of Commerson’s Anchovy (Stolephorus commersonnii) in Segara Anakan Cilacap Central Java Inferred from PCR-RFLP Markers

“…We were convincingly assigned that our PCR products were our target fragments of the COI gene rather than pseudo-gene. This belief was based on the result of sequences verification through BLAST method which was performed in previous study by Nuryanto et al (2017).…”

“…According to Nuryanto et al (2017) restriction enzyme only able to recognize a specific sequence. The recognizable site will be digested by the enzyme and produce RFLP markers.…”

“…This also means that no polymorphic locus was observed. According to Nuryanto et al (2017) a locus referred as polymorphic when the most common allele has frequency less than 95%.…”

“…Fish samples were collected during the field trips in April 2016. Samplings were done following the sampling techniques as explained by Nuryanto et al (2017). The obtained fish samples were preserved in 96% technical ethanol (Bratachem).…”

“…This marker cannot observe any variation in any sequences outside the restriction sites which can be observed on other genetic markers, such as COI sequences. It was reported by Nuryanto et al (2017) that genetic variation was observed in commerson's anchovy population in Segara Anakan when assessed using COI sequences.…”