Genetic transformation of strawberry: Stable integration of a gene to control biosynthesis of ethylene

Mathews, Helena; Wagoner, Wendy; Kellogg, J.; Bestwick, Richard K.

doi:10.1007/bf02632224

Cited by 49 publications

(25 citation statements)

References 30 publications

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“…Another possibility was that the overgrowth of untransformed calli prevented the development of transformed calli. The results from our experiments with 'Alpine' were similar to those of Mathews et al (1995) who reported that a high frequency of transformed shoots was observed when explants were initially exposed to 25 mg l À1 Kn compared to an initial exposure of 10 mg l À1 Kn in transformation of strawberry cultivars. We assumed that suppressing proliferation of untransformed cells could hasten shoot regeneration of PI 551572.…”

Section: Analysis Of Transgenic 'Alpine' Plantssupporting

confidence: 91%

High-efficiency transformation of the diploid strawberry (Fragaria vesca) for functional genomics

Oosumi

Gruszewski

Blischak

et al. 2005

Planta

108

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Fragaria vesca L., a diploid (2n = 2x = 14) relative of the commercial octoploid strawberry, is an attractive model for functional genomics research in Rosaceae. Its small genome size, short reproductive cycle, and facile vegetative and seed propagation make F. vesca a promising candidate for forward and reverse genetics experiments. However, the lack of a high-efficiency transformation protocol required for systematic production of thousands of T-DNA insertional mutant lines and high-throughput gene validation is a major bottleneck. We describe a new transformation procedure that uses leaf explants from newly unfolded trifoliate leaves obtained from stock plants 6-7 weeks after seed germination, co-cultivation with Agrobacterium strain GV3101, and stringent selection on MS medium containing 4 mg l(-1) hygromycin. Using this protocol we achieved 100% transformation efficiency for 6 of 14 F. vesca accessions tested. Accession PI 551572 was determined to be the best candidate for a model in F. vesca functional genomics research, as it showed the greatest propensity for callus formation, transformation, shoot regeneration, ex vitro establishment, and plant growth, requiring only 14-15 weeks to complete its life cycle in different seasons in the greenhouse.

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Section: Analysis Of Transgenic 'Alpine' Plantssupporting

confidence: 91%

High-efficiency transformation of the diploid strawberry (Fragaria vesca) for functional genomics

Oosumi

Gruszewski

Blischak

et al. 2005

Planta

108

View full text Add to dashboard Cite

show abstract

“…During the 1990s, reliable protocols for the genetic transformation of strawberry were established for several commercial cultivars (Barceló et al 1998;James et al 1990;Mathews et al 1995). Recently, these have been successfully employed to modify selected traits like insect resistance (Watt et al 1999), herbicide tolerance (Morgan et al 2002) and fruit softening (JimØnez-Bermffldez et al 2002).…”

Section: Introductionmentioning

confidence: 99%

TheCaMV 35S promoter is highly active on floral organs and pollen of transgenic strawberry plants

et al. 2004

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We have evaluated the expression of the reporter beta-glucuronidase (GUS) gene driven by the cauliflower mosaic virus 35S (CaMV 35S) promoter in flowers and pollen from 14 independent transgenic strawberry lines. Of the 14 lines evaluated, 13 (92.8%) showed GUS activity--as estimated by the histochemical GUS assay--in some floral organs, with expression being most common in the flower stem, sepals, petals, ovary and stigma. Ten of these thirteen transgenic lines (77%) showed GUS activity in pollen, although the percentages of positive pollen per flower varied greatly among the different lines. A study of the GUS expression during pollen maturation showed that the (CaMV 35S) promoter showed low expression in pollen from flower buds before anthesis but was activated in mature pollen following anther dehiscence. The percentages of pollen grains that showed GUS activity ranged from 2.1% to 46.3%. These percentages were similar or even higher when mature pollen was stored dry at room temperature for 2 weeks. After 5 weeks of storage, the percentages of GUS-positive pollen decreased in two of the six lines analysed but remained at similar values in the other four lines. GUS activity was also measured in protein extracts of mature pollen by means of the fluorometric GUS assay, with the values obtained ranging from 3.8 micromol MU mg protein(-1) h(-1) to 0.26 micromol MU mg protein(-1) h(-1). Contrary to the generally held view that the CaMV 35S promoter is virtually silent in pollen, we conclude that it is highly expressed in transgenic strawberry pollen.

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“…fragariae and Verticillium dahliae Kleb. [Mathews et al 1995[Mathews et al , Żebrowska 2011. With genetic engineering and use of appropriate regenerative methods, resistance to Fusarium oxysporum f. sp.…”

Section: Propagation Of Fragaria Plantsmentioning

confidence: 99%

“…The plant breeders main objective is to obtain plants resistant to diseases causing a huge economic losses. The biotechnological methods that use horizontal gene transfer and clonal propagation are practical means to stable one (or more) dominant gene transfer to achieve desirable plant traits [Mathews et al 1995].…”

Section: Propagation Of Fragaria Plantsmentioning

confidence: 99%

In vitro REGENERATION OF Fragraria PLANTS

Rokosa

Mikiciuk

2017

Acta Sci. Pol. Hortorum Cultus

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Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness -from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.

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Genetic transformation of strawberry: Stable integration of a gene to control biosynthesis of ethylene

Cited by 49 publications

References 30 publications

High-efficiency transformation of the diploid strawberry (Fragaria vesca) for functional genomics

High-efficiency transformation of the diploid strawberry (Fragaria vesca) for functional genomics

TheCaMV 35S promoter is highly active on floral organs and pollen of transgenic strawberry plants

In vitro REGENERATION OF Fragraria PLANTS

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