Genetic toxicology testing of 41 industrial chemicals

Dean, Brian; Brooks, T.M.; Hodson-Walker, G.; Hutson, D. H.

doi:10.1016/0165-1110(85)90005-3

Cited by 123 publications

(51 citation statements)

References 34 publications

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“…The stocks were kept at -80°C. Cytotoxic doses of dicaphton (10.000, 1.000, 100, 10, 1 and 0.1 lg/plate) were determined by the method of Dean et al (1985).…”

Section: Ames Plate Incorporation Testmentioning

confidence: 99%

Mutagenicity and genotoxicity of dicapthon insecticide

Liman

2014

Cytotechnology

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Mutagenic and genotoxic effects of dicapthon were investigated by using the bacterial reverse mutation assay in Salmonella typhimurium TA97, TA98, TA100 and TA102 strains with or without metabolic activation system (S9 mix), and chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests in human peripheral blood lymphocytes in vitro. Dicapthon was dissolved in dimethyl sulfoxide for all test systems. 0.1, 1, 10 and 100 lg/plate doses of dicapthon were found to be weakly mutagenic on S. typhimurium TA 98 without S9 mix. The human peripheral lymphocytes were treated with four experimental concentrations of dicapthon (25, 50, 100, and 200 lg/mL) for 24 and 48 h. Dicapthon increased the frequency of SCE only at the 100 lg/mL concentration for the 24 and 48 h applications. Dicapthon also induced abnormal cell frequency, CA/cell ratio and frequency of MN dose dependently for 24 and 48 h. Dicapthon showed a statistically significant cytotoxic effect by decreasing the mitotic index in all concentrations and a cytostatic effect by decreasing nuclear division index in 100 and 200 lg/mL concentrations for both treatment periods when compared with both untreated and solvent controls. These values decreased also in a dose dependent manner.

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“…The stocks were kept at -80°C. Cytotoxic doses of dicaphton (10.000, 1.000, 100, 10, 1 and 0.1 lg/plate) were determined by the method of Dean et al (1985).…”

Section: Ames Plate Incorporation Testmentioning

confidence: 99%

Mutagenicity and genotoxicity of dicapthon insecticide

Liman

2014

Cytotechnology

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“…Cytotoxic doses of Lg-ext (3000, 1500, 750, 375, 187.5 and 93.75 mg/plate) were determined by following the method of Dean et al (1985).…”

Section: Ames Plate Incorporation Testmentioning

confidence: 99%

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Liman¹,

Gökçe²,

Akyıl³

et al. 2012

Rev. bras. farmacogn.

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Genotoxic and mutagenic effects of aqueous extract from aerial parts Linaria genistifolia (L.) Mill. subsp. genistifolia, Plantaginaceae (Lg-ext) were investigated by using both Allium cepa root meristematic cells and bacterial reverse mutation assay in Salmonella typhimurium TA98 and TA100 with or without metabolic activation system (S9), respectively. In Allium root growth inhibition test, EC50 value was determined approximately 15 g/L and 0.5xEC50, EC50 and 2xEC50 concentrations of Lg-ext were introduced to onion tuber roots and distilled water and methyl methane sulfonate (MMS, 10 ppm) used as a negative and positive control, respectively. The characteristic effect caused by tested preparations was an increase of mitotic index (MI) in 7.5 g/L and 15 g/L (except 24 and 96 h) and simultaneous decrease of MI in 30 g/L and in MMS. While stickiness, bridges, chromosome laggards and disturbed anaphase-telophase were observed in anaphase-telophase cells, c-metaphase, pro-metaphase, polyploidy and binuclear cells were observed in other cells. Lg-ext was not found to be mutagenic on S. typhimurium TA 98 and TA100 with or without S9. The results were also analyzed statistically by using SPSS for Windows, and Duncan's multiple range tests were performed respectively. These results indicate that Lg-ext exhibits genotoxic activity in A. cepa root meristematic cells but not mutagenic activity in Ames test system.

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“…• C cytotoxicity assessment was made (Dean et al 1984). Tester strains Salmonella typhimurium TA98 and TA100 strains were kindly provided by Dr. Bruce Ames (Biochemistry Department, University of California, Berkeley, CA, USA).…”

Section: Test Materials and Sample Preparationmentioning

confidence: 99%

Mutagenic activities of ten imidazole derivatives in Salmonella typhimurium

Aydoğan

Kutlu

2007

Biologia

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Ten imidazole derivatives were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100 both in the absence and presence of metabolic activation by the microsomal fraction S9 mix. In a general manner, derivatives tested exhibited a greater mutagenic activity in the TA100 strain comparing to the responses in TA 98. In the standard plate incorporation assay, 8 of these substances (80%) were found to be mutagenic for at least one of the two strains in the presence or absence of metabolic activation. Two compounds showed positive results in TA98 and 6 compounds were also mutagenic in TA100 without S9. In the presence of S9 mix, all of the 10 substances were non-mutagenic in TA98, whereas 4 compounds were positive in TA100. The results suggested the mutagenic potentials of the imidazole derivatives particularly inducing the reversion of base-pair substitutions. According to the structure-activity relationships phenyl groups in position 2 with different substituents can confer the mutagenic activity of the tested compounds. Methyl groups in different positions of these phenyl substituents can cause different types of mutations. This mutagenic effect is observed more clearly when the phenyl group is inhibited with a nitro group.

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Genetic toxicology testing of 41 industrial chemicals

Cited by 123 publications

References 34 publications

Mutagenicity and genotoxicity of dicapthon insecticide

Mutagenicity and genotoxicity of dicapthon insecticide

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Mutagenic activities of ten imidazole derivatives in Salmonella typhimurium

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