Genetic structure of the Greek olive germplasm revealed by RAPD, ISSR and SSR markers

Linos, A.; Nikoloudakis, Nikolaos; Katsiotis, Andreas; Hagidimitriou, Marianna

doi:10.1016/j.scienta.2014.05.034

Cited by 48 publications

(35 citation statements)

References 66 publications

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“…In fact, various types of molecular markers have been widely used to distinguish between olive varieties, determine cultivars origin and to study the genetic diversity (De Caraffa et al, 2002;Rao Durgac et al, 2010;Rekik et al, 2008). Among these molecular markers, Microsatellites are one of the most used for olive genotyping and cultivars discrimination (Diaz et al, 2006Sarri et al, 2006Baldoni et al, 2009;Bracci et al, 2009;Erre et al, 2010;Díez et al, 2011;Fendrin et al, 2014;Linos et al, 2014). Moreover, SSR markers are suited to distinguish closely related genotypes because of their high degree of variability and multi-allelic states.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among Algerian olive (Olea europaea L.) cultivars using SSR marker.

Abdessemed¹,

Muzzalupo

Benbouza

2015

Scientia Horticulturae

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Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among Algerian olive (Olea europaea L.) cultivars using SSR marker.

Abdessemed¹,

Muzzalupo

Benbouza

2015

Scientia Horticulturae

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“…These technologies were successfully used for DNA-fingerprinting of a wide range of crop species and to discriminate between closely related genotypes (Fang and Roose, 1997;Gilbert et al, 1999;Ghariani et al, 2003b;Yao et al, 2008). In some cases, molecular data were compared and integrated with phenotypic data to evaluate their utility as a tool for germplasm conservation and plant breeding (Russell et al, 2000;Muthusamy et al, 2008;Krichen et al, 2010;Linos et al, 2014). The salient features of this work were (i) a comparative analysis using both RAPD and ISSR markers, (ii) the evaluation of the genetic relatedness among molecular and morpho-agronomical markers and (iii) the assessment of genetic diversity between Tunisian spontaneous species and introduced germplasm ryegrass cultivars.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

Ghariani¹,

Elazreg²,

Chtourou-Ghorbel³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Tunisia is rich in diverse forage and pasture species including perennial ryegrass. In order to enhance forage production and improve agronomic performance of this local germplasm, a molecular analysis was undertaken. Random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and morpho-agronomical traits markers were used for genetic diversity estimation of ryegrass germplasm after screening 20 spontaneous accessions, including a local and an introduced cultivars. Same mean polymorphism information content values were obtained (0.37) for RAPD and ISSR suggesting that both marker systems were equally effective in determining polymorphisms. The average pairwise genetic distance values were 0.57 (morpho-agronomical traits), 0.68 (RAPD), and 0.51 (ISSR) markers data sets. A higher Shannon diversity index was obtained with ISSR marker (0.57) than for RAPD (0.54) and morphoagronomical traits (0.36). The Mantel test based on genetic distances of a combination of molecular markers and morpho-agronomical data exhibited (2015) a significant correlation between RAPD and ISSR data, suggesting that the use of a combination of molecular techniques was a highly efficient method of estimating genetic variability levels among Tunisian ryegrass germplasm. In summary, results showed that combining molecular and morpho-agronomical markers is an efficient way in assessing the genetic variability among Tunisian ryegrass genotypes. In addition, the combined analysis provided an exhaustive coverage for the analyzed diversity and helped us to identify suitable accessions showed by Beja and Jendouba localities, which present large similarities with cultivated forms and can be exploited for designing breeding programmes, conservation of germplasm and management of ryegrass genetic resources.

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“…The use of RAPD markers has been extremely common, alone or in combination with other markers (AFLPs, ISSRs, SSRs), to analyse the extent of genetic variability present in the germplasm of varieties of countries characterised by an old history of olive cultivation and oil production: Malta (Mazzitelli et al 2014), Jordan (Brake et al 2014), Greece (Linos et al 2014), Egypt (Elsheikh et al 2014), Iran (Sheidai et al 2014), Pakistan (Awan et al 2011), and Turkey (Coskun and Parlak 2013). In other papers, RAPD markers have been considered to study the potential presence of intra-cultivar variability (Ç elikkol Akçay et al 2014;Figueiredo et al 2013;Leva and Petruccelli 2012), the genetic relationships between cultivars (Gomes et al 2012), the genetic characterisation of minor cultivars in local regions (Parra-Lobato et al 2012), the results of in vitro mutagenesis, and the detection of possible mutants in olive calli caused by the use of sodium azide (Alborzian Deh Sheikh and Moradnejad 2014).…”

Section: Rapds Issrs and Aflpsmentioning

confidence: 99%

“…In addition to other markers, ISSR have been used to study and characterise the genetic variability in local olive germplasm (Zhan et al 2015;Brake et al 2014;Elsheikh et al 2014;Linos et al 2014;Leva and Petruccelli 2012;Noormohammadi et al 2012) Further, Kaya and Yilmaz-Gokdogan (2016) have used ISSRs in addition to two retrotransposon-based marker systems (IRAPinter-retrotransposon amplified polymorphism and REMAPretrotransposon-microsatellite amplified polymorphism) for the molecular characterisation of olive cultivars.…”

Section: Rapds Issrs and Aflpsmentioning

confidence: 99%

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

Sebastiani

Busconi

2017

Plant Cell Rep

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The latest results in DNA markers application and genomic studies in olive. Olive (Olea europaea L.) is among the most ancient tree crops worldwide and the source of oil beneficial for human health. Despite this, few data on olive genetics are available in comparison with other cultivated plant species. Molecular information is mainly linked to molecular markers and their application to the study of DNA variation in the Olea europaea complex. In terms of genomic research, efforts have been made in sequencing, heralding the era of olive genomic. The present paper represents an update of a previous review work published in this journal in 2011. The review is again mainly focused on DNA markers, whose application still constitutes a relevant percentage of the most recently published researches. Since the olive genomic era has recently started, the latest results in this field are also being discussed.

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Genetic structure of the Greek olive germplasm revealed by RAPD, ISSR and SSR markers

Cited by 48 publications

References 66 publications

Assessment of genetic diversity among Algerian olive (Olea europaea L.) cultivars using SSR marker.

Assessment of genetic diversity among Algerian olive (Olea europaea L.) cultivars using SSR marker.

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

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