Genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration of Carum copticum L.

Niazian, Mohsen; Noori, Seyed Ahmad Sadat; Galuszka, Petr; Tohidfar, Masoud; Mortazavian, Seyed Mohammad Mahdi

doi:10.1016/j.indcrop.2016.12.044

Cited by 53 publications

(23 citation statements)

References 22 publications

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“…Our flow cytometry results did not identify any problems resulting from the use of plant growth regulators, culture conditions, or the regeneration system used in producing walnut SEs. Genome size similarity and fidelity were also reported in previous studies of regeneration from somatic embryos of other species (Niazian et al, 2017;Pinto et al, 2004;Viehmannova et al, 2016). Molecular analysis.…”

Section: Resultssupporting

confidence: 73%

“…Molecular analyses and flow cytometry have been used in many species to evaluate the trueness-to-type of plantlets regenerated via somatic embryogenesis. These species include olives (Brada€ ı et al, 2016), muskmelon (Raji et al, 2018), chrysanthemum (Lema-Rumi nska and Sliwi nska, 2015), ajowan (Niazian et al, 2017), Italian grapevine (Carra et al, 2016), Anthurium andraeanum (Bhattacharya et al, 2016), citrus species (Meziane et al, 2017), and Cassia occidentalis (Naz et al, 2016). In walnut, RFLP and isozyme analysis were used to determine the origin of SEs by Aly et al (1992).…”

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confidence: 99%

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Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Sadat-Hosseini¹,

Vahdati²,

Leslie³

2019

horts

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Somatic embryos (SEs) can play important roles in genetic manipulation and breeding. They can be used as targets for induced mutagenesis, as material for cryopreservation and germplasm conservation, and for transformation or gene editing in support of plant improvement and proof of gene function. However, germination rates of walnut (Juglans regia) SEs are low, and the genetic stability of plantlets regenerated from them has not been explored. Here, we studied first the effects of gibberellic acid (GA3) and low temperature storage (LTS) on germination of walnut somatic embryos. Second, we assessed the genetic fidelity of plantlets regenerated from these SEs by comparing them to each other and to their cultivar of origin. Results showed that GA3 and LTS increased the walnut SE germination rate. The best rate was observed when SEs were subjected to LTS for 60 d followed by culture on a medium with either 1 or 3 mg·L−1 GA3 (56.6% and 46.6% germination respectively). Genetic stability was evaluated, using flow cytometry and 15 sets of ISSR primers. Flow cytometry indicated that all samples (i.e., regenerated and parental counterpart) showed the same peak. Amplified fragments of inter simple sequence repeats (ISSR) primers ranged in size from ≈200 to 1800 bp. All ISSR profiles of regenerants were monomorphic. Results did not show any genetic differences among plantlets regenerated from SEs or from their parental counterpart. Due to this apparent genetic stability, walnut SEs can be useful for genetic transformation and germplasm conservation.

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Section: Resultssupporting

confidence: 73%

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confidence: 99%

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Sadat-Hosseini¹,

Vahdati²,

Leslie³

2019

horts

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“…The effect of different PGRs on callus induction. In vitro plant regeneration from different parts of in vitro-grown seedlings has received notable attention, and many researchers have used various parts of in vitrogrown seedling as explants (Jafari et al, 2017;Mali and Chavan, 2016;Niazian et al, 2017;Phulwaria et al, 2013;Singh et al, 2016) to improve the micropropagation method for many plant species. This study demonstrated the candidature of hypocotyl segments, which are obtained from axenic seedling, as a source of explant for in vitro regeneration of F. religiosa for the first time ( Fig.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant

Hesami

Daneshvar

2018

horts

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Ficus religiosa is an important industrial, medicinal, and ornamental plant, so in vitro regeneration is of high paramount in this valuable germplasm. Two efficient protocols were developed for indirect and direct shoot organogenesis through hypocotyl explants. In the first experiment, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and indole butyric acid (IBA) (0.5, 1.0, and 1.5 mg·L−1) in combination with 6-benzyl amino purine (BAP) (ratio 10:1, respectively) were used for callus formation. Two types of callus were obtained from different concentrations of plant growth regulators (PGRs). Also, 2,4-D produced yellow-brownish and friable callus (Type I), whereas the green and compact callus (Type II) was achieved in IBA. The highest callus fresh weight (2.43 g) was observed in Murashige and Skoog (MS) medium containing 0.5 mg·L−1 2,4-D plus 0.05 mg·L−1 BAP. In the later experiments, various concentrations of thidiazuron (TDZ), 6-furfuryl amino purine (KN), and BAP (0.25, 0.5, 1.0, and 1.5 mg·L−1) in combination with IBA (ratio 10:1, respectively) were applied for shoot regeneration (direct and indirect organogenesis). In shoot regeneration from callus, the highest regeneration frequency (86.66%) and shoot number per callus (4.13) were achieved in MS medium supplemented with 1.5 mg·L−1 BAP plus 0.15 mg·L−1 IBA from type I calli. However, no regeneration was observed in type II calli. In direct shoot regeneration, the highest regeneration frequency (96.66%) and shoot number (6.26) were obtained in the medium mentioned previously. In root induction experiment, different concentrations of naphthalene acetic acid (NAA) and IBA alone or in combination were applied, and MS medium containing 2.0 mg·L−1 IBA along with 0.1 mg·L−1 NAA was the best hormonal balance for root induction. The rooted plantlets’ survival rate was more than 95% in the acclimatization stage. These results demonstrated that the direct regeneration method provides more shoot regeneration frequency and take a less time for shoot organogenesis than the indirect regeneration method. Based on our knowledge, this study is the first report of direct and indirect shoot organogenesis of F. religiosa via hypocotyl from in vitro–grown seedling.

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“…However, when the callus is placed in suitable and controlled conditions, the differentiation of shoots, embryos, and roots can be achieved. Based on these culture systems, various studies on biochemical or cytological processes of growth attributed to cell enlargement, differentiation, and cell division have been conducted (Bakhshaie et al, 2016;Daneshvar, 2016a, 2016b;Jalali et al, 2012;Niazian et al, 2017). Moreover, various reactions of genotypes from one species to in vitro culture conditions have been observed regularly and has been explained to be due to variations in endogenous phytohormone levels or abilities to react differently to exogenously applied plant growth regulators (PGRs) (Bhojwani and Dantu, 2013;Hesami et al, 2017b).…”

Section: Callus Culturementioning

confidence: 99%

In vitro culture as a powerful method for conserving Iranian ornamental geophytes

Hesami¹,

Naderi²,

Yoosefzadeh-Najafabadi³

et al. 2018

bta

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Never before in history has the issue of preserving biodiversity been of such significance than today, to the extent that it has become an important global issue. Nowadays, humans have a massive impact on biodiversity whether in rural, urban, or wilderness settings. Besides, biodiversity today is also being significantly constrained by climate change and the introduction of new varieties of plants. Plant tissue culture is a method that can be useful for collecting, storing, and multiplicating of plant germplasms. Nowadays, many valuable germplasms are in danger of extinction, especially in Iran. Thus, there is a necessity to preserve valuable Iranian ornamental geophytes such as Oxalis articulata, Eminium jaegeri, Muscari kurdicum, Leopoldia tijtijensis, Gagea calcicola, Tulipa faribae, Gagea alexii, and Allium. In this study, plant tissue culture as well as in vitro conservation techniques as means for medium-and long-term conservation of Iranian ornamental geophytes is reviewed. There are only few studies on plant tissue culture of Iranian ornamental geophytes despite their great importance in Iran. To our knowledge, there is no report on cryopreservation of the abovementioned plants. In conclusion, the methods presented in this review can be utilized for conserving these valuable germplasms for future generations.

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Genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration of Carum copticum L.

Cited by 53 publications

References 22 publications

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant

In vitro culture as a powerful method for conserving Iranian ornamental geophytes

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