Genetic similarity, antibiotic resistance and disinfectant susceptibility of Listeria monocytogenes isolated from chicken meat and chicken-meat processing environment in Mato Grosso, Brazil

Carvalho, Fernanda Tavares; Vieira, Bruno Serpa; Vallim, Deyse Christina; Carvalho, Larrayane Albuês; Carvalho, Ricardo César Tavares; Pereira, Rodrigo C.L.; Figueiredo, Eduardo Eustáquio de Souza

doi:10.1016/j.lwt.2019.03.099

Cited by 25 publications

(20 citation statements)

References 42 publications

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“…Lineages I and II have been identified in the great majority of the clinical cases of listeriosis in humans [37,38], especially the serotype 1/2a (lineage II) which is also the most prevalent in food [39,40]. In a previous study by our research group, LM 1/2a was the predominant serotype (94.6%) isolated from samples of chicken meat and chicken meat processing environment in Mato Grosso [41]. In the present study, no serotype 1/2a was identified in beef from Mato Grosso.…”

Section: Discussionmentioning

confidence: 83%

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

et al. 2019

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The Brazilian state of Mato Grosso is the largest producer and exporter of beef in the country, but few studies of relevance have been conducted to evaluate the microbiological safety of its products. This study aimed to estimate the prevalence of Listeria monocytogenes (LM) in export-approved beef from Mato Grosso and to characterize the isolates in terms of molecular properties and antimicrobial resistance. From a total of 50 samples analyzed, Listeria sp. was isolated in 18 (36% prevalence). Listeria monocytogenes was confirmed in 6 (12% prevalence). Among the serotype groups assessed by multiplex PCR, serotype 4 (4b, 4d or 4e) was the most prevalent. Although antibiotic resistance was not an issue, two strains isolated from different plants showed high resistance to sodium hypochlorite. Overall, this scenario causes concern because it puts at risk not only the Brazilian customer, but also the population of countries that import beef from Mato Grosso.

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Section: Discussionmentioning

confidence: 83%

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

et al. 2019

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“…In the present study, 71.8% of L. monocytogenes isolates were detected as serotype 1/2a. In other studies, Carvalho et al [29] reported mostly 1/2a (94.6%) in chicken meat and chicken-meat processing environment, Oliveira et al [30] identified 87% of the L. monocytogenes isolates as 1/2a in samples of chicken carcasses, and Zeinali et al [31] determined that 52.77% of L. monocytogenes 1/2a serotype were predominant in chicken carcasses, followed by 4a and 4c serotypes (27.77%) in İran. Arslan and Baytur [32] revealed 57.6% of L. monocytogenes strains isolated from chicken meat were 1/2a.…”

Section: Discussionmentioning

confidence: 83%

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Gücükoğlu¹,

Çadırcı²,

Gülel³

et al. 2020

Kafkas Univ Vet Fak Derg

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In this study, 240 organic chicken pieces (80 thighs, 80 wings, 80 skinless-breast meat) were analyzed for the presence of Listeria monocytogenes. Within the framework of the analysis findings; L. monocytogenes was detected in 60 (25%) of all 240 collected samples. In particular, L. monocytogenes was detected in 24 (30%) of 80 thigh samples, 20 (25%) of 80 wing samples and 16 (20%) of 80 skinless-breast meat samples. Serotyping distribution of 96 L. monocytogenes isolates determined as 71.8% serotype 1/2a, 21.9% serotype 1/2b, 4.2% serotype 4b and 2.1% serotype 1/2c. According to antibiotic resistance profile, 26 isolates (27%) were found to be resistant to ampicillin. The other isolates were found to be resistant to meropenem, tetracycline, sulfamethoxazole/trimethoprim, penicillin G, amoxicillin/clavulanic acid, vancomycin, oxytetracycline, erythromycin and chloramphenicol as 23 (23.9%), 14 (14.5%), 13 (13.5%), 12 (12.5%), 9 (9.3%), 7 (7.2%), 5 (5.2%), 4 (4.1%) and 3 (3.1%) respectively. Multiple antibiotic resistance profiles were determined in 12 of L. monocytogenes isolates. The findings of this study are thought to be unique data for serotyping studies that will help in revealing the epidemiology of L. monocytogenes in organic poultry meat, enterprises operating in food sector and diagnosis and treatment of listeriosis.

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“…On the basis of the results of the Rapid Alert System for Food and Feed (RASFF) investigation, it can be stated that the products of animal origin are mostly linked to L. monocytogenes infections [9]. Raw poultry and meats, processed meat, and RTE meat products can be contaminated with L. monocytogenes in the slaughterhouse or on different processing steps [6,[10][11][12][13]. It is suggested that most of the meat processing plants may be colonized by L. monocytogenes as soon as during initial production cycles as an effect of raw meat contamination or improper hygienic conditions in the food processing environment (FPE) [1,14].…”

Section: Introductionmentioning

confidence: 99%

“…Since L. monocytogenes pathogenicity and persistence in FPE may be related to its serotype, the serological identification of L. monocytogenes strain isolated from food is of major importance for consumer safety [1]. About 90-95% of the strains isolated from contaminated food, animal, and human samples belong to serotypes 1/2a, 1/2b, 1/2c, and 4b, among which mentioned serotype 4b is responsible for the majority of human listeriosis cases and 1/2a serotype is the most prevalent in foods [1,4,6,7,10,17,19].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

Skowron

Wałecka-Zacharska

Wiktorczyk-Kapischke

et al. 2020

Foods

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Listeria monocytogenes are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated L. monocytogenes. Between 2016–2018, 6000 swabs were taken (2000 annually) from the surface of pork, beef, and poultry. The analysis of intermediate and finished product samples was carried out in accordance with ISO 11290-1 (International Organization for Standardization). The genetic similarity assessment of the isolates obtained was based on the Pulsed Field Gel Electrophoresis (PFGE) method, and drug-sensitivity assessment using the disc-diffusion method. We found 2.1% of collected samples were L. monocytogenes positive. The level of meat contamination varied depending on its matrix. Most L. monocytogenes were isolated from poultry. It was shown that 39 (32.5%) strains were sensitive to all tested antibiotics and eight (6.7%) were resistant to all five tested antimicrobials. Most strains tested were resistant to cotrimoxazole (55; 45.8%) and meropenem (52; 43.3%), followed by erythromycin (48; 40.0%), penicillin (31; 25.8%), and ampicillin (21; 17.5%). High prevalence of this pathogen may be a serious problem, especially when linked with antibiotic resistance and high percentage of serotypes responsible for listeriosis outbreaks.

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Genetic similarity, antibiotic resistance and disinfectant susceptibility of Listeria monocytogenes isolated from chicken meat and chicken-meat processing environment in Mato Grosso, Brazil

Cited by 25 publications

References 42 publications

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

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