Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

Abstract

In this study, 240 organic chicken pieces (80 thighs, 80 wings, 80 skinless-breast meat) were analyzed for the presence of Listeria monocytogenes. Within the framework of the analysis findings; L. monocytogenes was detected in 60 (25%) of all 240 collected samples. In particular, L. monocytogenes was detected in 24 (30%) of 80 thigh samples, 20 (25%) of 80 wing samples and 16 (20%) of 80 skinless-breast meat samples. Serotyping distribution of 96 L. monocytogenes isolates determined as 71.8% serotype 1/2a, 21.9% serotype 1/2b, 4.2% serotype 4b and 2.1% serotype 1/2c. According to antibiotic resistance profile, 26 isolates (27%) were found to be resistant to ampicillin. The other isolates were found to be resistant to meropenem, tetracycline, sulfamethoxazole/trimethoprim, penicillin G, amoxicillin/clavulanic acid, vancomycin, oxytetracycline, erythromycin and chloramphenicol as 23 (23.9%), 14 (14.5%), 13 (13.5%), 12 (12.5%), 9 (9.3%), 7 (7.2%), 5 (5.2%), 4 (4.1%) and 3 (3.1%) respectively. Multiple antibiotic resistance profiles were determined in 12 of L. monocytogenes isolates. The findings of this study are thought to be unique data for serotyping studies that will help in revealing the epidemiology of L. monocytogenes in organic poultry meat, enterprises operating in food sector and diagnosis and treatment of listeriosis.

Microbiological safety of food of animal origin from organic farms

“…Similarly, Gücükoğlu et al (16) demonstrated the presence of L. monocytogenes in 60 out of 240 (25%) organic chicken meat samples. Most of the isolates were of the 1/2a serogroup (71.9%), followed by 1/2b (21.8%), 4b (4.2%) and 1/2c (2.1%) (16). The 1/2a serogroup is considered to be the most prevalent in food among all L. monocytogenes identified (48).…”

“…Most of the bacteria were of the 4b serogroup, which is responsible for the majority of human listeriosis outbreaks (12,48). Similarly, Gücükoğlu et al (16) demonstrated the presence of L. monocytogenes in 60 out of 240 (25%) organic chicken meat samples. Most of the isolates were of the 1/2a serogroup (71.9%), followed by 1/2b (21.8%), 4b (4.2%) and 1/2c (2.1%) (16).…”

Global trends in antimicrobial resistance on organic and conventional farms

“…These trends in organic farming carry important implications for disease outbreaks and the spread of antimicrobial resistance. Indeed, mounting evidence suggests that organic farming practices can reduce the occurrence of pathogenic outbreaks and, therefore, the presence of genes that carry and spread antimicrobial resistance [9][10][11] . These patterns suggest a potential win-win for animal welfare, conservation, and public health that move far beyond stewardship policies that vary widely across geographic regions and can unintentionally drive an increase in antimicrobial use.…”