Genetic resistance to Colletotrichum lindemuthianum in the Andean cultivar Jalo Pintado 2 of common bean

Abstract

The anthracnose caused by fungus Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara is the most widespread disease and economically important fungal disease of common bean (Phaseolus vulgaris L.). The use of resistant cultivars is considered as one of the most effective methods in controlling this disease. The present study had as aim to characterize the genetic resistance of the Andean common bean cultivar Jalo Pintado 2 to the C. lindemuthianum through inheritance and allelism tests. The experiment was conducted under greenhouse conditions at Laboratório de Melhoramento do Feijão Comum e de Biologia Molecular do Núcleo de Pesquisa Aplicada a Agricultura (Nupagri) at Universidade Estadual de Maringá, Paraná, Brazil. The results of the F2 population from the crossing 'Jalo Pintado 2' (R) × Cornell 49-242 (S), inoculated with race 73 of C. lindemuthianum, adjusted to the ratio of 3R: 1S, demonstrating the action of a dominant gene in the cultivar Jalo Pintado 2. The allelism tests evidenced that the gene in the 'Jalo Pintado 2' is independent from those previously characterized: Co-1, Co-2,Co-3, Co-34, Co-4, Co-42, Co-43, Co-5, Co-6, Co-11, Co-12, Co-13, Co-14, Co-15 and Co-16. This gene is also independent from those genes not yet named present in Paloma, Perla and Amendoim Cavalo cultivars. The authors propose the Co-

Genetic resistance of common bean cultivar Beija Flor to Colletotrichum lindemuthianum

“…In the literature, we can observe similar results in studies that used the same parental (MDRK, Cornell 49-242, TO, AB 136, Jalo Vermelho, Pitanga, Corinthiano, Perla, and G 2333), as for example, the Paloma × Cornell 49-242 cross, also inoculated with race 65, where Castro et al (2017) found a segregation ratio of 15R:1S. Furthermore, Frias, Gonçalves-Vidigal, Nanami, Castro, and Vidigal Filho (2016) conducted testes on the F 2 populations from the Jalo Pintado 2 × Cornell 49-242, Jalo Pintado 2 × TO, and Jalo Pintado 2 × AB 136 crosses and reported a segregation ratio of 15R:1S. Likewise, Nanami et al (2017) also performed the inoculation of race 65 on the F 2 populations Amendoim Cavalo × TO and Amendoim Cavalo × AB 136.…”

“…Likewise, Nanami et al (2017) also performed the inoculation of race 65 on the F 2 populations Amendoim Cavalo × TO and Amendoim Cavalo × AB 136. Moreover, Frias et al (2016) obtained similar results evaluating F 2 populations derived from the Jalo Pintado 2 × Jalo Vermelho, Jalo Pintado 2 × Pitanga Jalo, and Pintado 2 × Corinthiano crosses. Inheritance studies conducted by Castro et al (2017), using the F 2 populations derived from the Paloma × Jalo Vermelho, Paloma × Pitanga, and Paloma × Corinthiano crosses reported the segregation of 15R:1S, providing evidence of the action of two dominant genes.…”

Characterization of genetic resistance in Andean common bean cultivar Amendoim Cavalo to Colletotrichum lindemuthianum

“…Likewise, the same result was observed in the F2 generations of crosses between Amendoim Cavalo × Paloma (X² = 0.009; p = 0.924), Amendoim Cavalo × Perla (X² = 0.244; p = 0.621) and Amendoim Cavalo × Jalo Pintado 2 (X² = 0.011; p = 0.918). Therefore, by allelism tests, it was confirmed that the gene in 'Amendoim Cavalo' is independent from Co-Pa (Lima-Castro et al, 2017), Co-Pe and Co-18 (Frias et al, 2016), respectively.…”

“…In order to characterize the resistance genes to C. lindemuthianum present in 'Amendoim Cavalo' (Figure 1), F2 segregating populations and F2:3 families obtained from crossings of 'Amendoim Cavalo' and differential cultivars proposed by Pastor-Corrales et al (1991) PI 207262, TO, TU, AB 136 and G 2333. Another crosses were also carried out with cultivars that have resistance genes to C lindemuthianum already identified: Ouro Negro, Jalo Vermelho, Jalo Listras Pretas, Pitanga, Corinthiano, Crioulo 159, Paloma, Jalo Pintado 2 (Frias et al, 2016) and Perla.…”

Genetic diversity of Colletotrichum lindemuthianum races based on ITS-rDNA regions

“…Anthracnose resistant cultivars is the most cost-effective and sustainable strategy to control anthracnose in subsistence and commercial farming (Pastor-Corrales & Tu, 1989;Sileshi et al, 2014;Frias et al, 2016;Nanami et al, 2017). The durability of the cultivar resistance is often affected by the emergence of new virulent races of C. lindemuthianum, wich can overcome the resistant cultivar (Kelly, Afanador & Cameron, 1994;Del Río, Lamppa, Gross, Brolley & Prischmann, 2003).…”