Genetic relationships between two homologous goat breeds from Portugal and Brazil assessed by microsatellite markers

Oliveira, Julio Cezar M.; Ribeiro, Maria Norma; Rocha, Leandro Branco; Gomes-Filho, M.A.; Delgado, Juan Vicente; Martínez-Martínez, A.; Menezes, Marcos Paulo Carrera; Bettencourt, Conceição; Gama, L. T.

doi:10.1016/j.smallrumres.2010.05.002

Cited by 20 publications

(24 citation statements)

References 38 publications

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“…In Brazil, a few studies have recently addressed the existence of local breeds using either microsatellites or mtDNA, and all have focused on those from the northern region of the country, for example the Canindé, Graúna, Marota, Moxotó, Repartida, and Serrana Azul (Araújo et al, 2006;Menezes et al, 2006;Oliveira et al, 2007Oliveira et al, , 2010. Recently, a next-generation sequencing approach was employed to investigate genetic variation in Brazilian naturalized breeds (Moura et al, 2015).…”

Section: Crespa As a Distinct Genetic Clustermentioning

confidence: 99%

“…This is probably due to the high prevalence of this group of haplotypes in southern Europe, the major source of the breeds that are currently reared in southern Brazil. Although genetic relationships between Brazilian (specifically from the northern region of the country) and Portuguese breeds have already been addressed (Oliveira et al, 2010;Ribeiro et al, 2012), the degree of relatedness among foreign breeds reared in southernmost Brazil, particularly the locally adapted Crespa, has never been studied. We therefore inserted these breeds into the global scenario using worldwide haplogroups, native Portuguese breeds, and native Turkish Angora.…”

Section: Genetic Relatedness and Ancestrymentioning

confidence: 99%

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Ancestry analysis of locally adapted Crespa goats from southernmost Brazil

et al. 2016

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Section: Crespa As a Distinct Genetic Clustermentioning

confidence: 99%

Section: Genetic Relatedness and Ancestrymentioning

confidence: 99%

Ancestry analysis of locally adapted Crespa goats from southernmost Brazil

et al. 2016

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“…Therefore the loci examined in this study were highly informative. The mean number of alleles per loci (13.33) were higher than those reported by Saitbekova et al 38 , Agha et al 39 Genetic Diversity of Three ... et al 40 , Fatima et al 41 , Oliveira et al 42 , Qi et al 43 , lower than those reported by Serrano et al 33 , Caňón et al 44 and similar to those reported by Missohou et al 37 , Barker at al. 45 , Chenyambuga et al…”

Section: Discussionmentioning

confidence: 51%

“…, TadlaouiOuafai et al 62 , and lower than that reported by Oliviera et al 42 , Hassen et al 47 , Li et al 48 , Gour et al 51 , Dixit et al 63 and Kumar et al 64 . Luikart et al 1 and Naderi et al 3 have stated that a lower F ST value generally observed in goat populations results from a higher mobility of goats between different regions, which reduces a genetic differentiation between populations.…”

mentioning

confidence: 85%

Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Gürler¹,

Bozkaya²

2013

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the genetic variability of three goat populations raised in the Southeastern region of Turkey by using microsatellite loci defined in gazelle (Gazella granti). DNA was isolated from 120 blood samples collected from goats raised in Sanliurfa (n = 40), Kilis (n = 40) and Siirt (n = 40) provinces. Eight microsatellite loci (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) were used for amplification with polymerase chain reaction (PCR). Fragment lengths of the amplifed loci were analysed using a capillary electrophoresis system. Six of the eight loci (75%) were successfully amplified and four (66.7%) of them were polymorphic. Three of the polymorphic loci (33HDZ496, 33HDZ749 ve 33HDZ974) were used for estimating genetic parameters. A total of 40 alleles were detected. Number of alleles and polymorphism information contents (PIC) for the loci 33HDZ496, 33HDZ749 and 33HDZ974 Keywords: Goat, Microsatellite, Genetic diversity Güney Doğu Anadolu Bölgesi'nde Yetiştirilen Üç Keçi Populasyonunda Genetik Çeşitlilik ÖzetBu çalışma bir ceylan türünde (Gazella granti) tanımlanmış sekiz mikrosatelit lokusunu kullanarak Güneydoğu Anadolu Bölgesinde yetiştirilen üç keçi populasyonunu genetik çeşitlilik yönünden değerlendirmek amacıyla yapılmıştır. Bu amaçla, Şanlıurfa (n = 40), Kilis (n = 40) ve Siirt (n = 40) illerinde yetiştirilen üç keçi populasyonundan toplam 120 adet kan örneği alınmış ve bu örneklerden DNA izole edilmiştir. İzole edilen DNA'lar sekiz mikrosatelit lokusuna (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) ait primerler kullanılarak PCR ile çoğaltılmış ve PCR ürünleri kapillar elektroforeze tabi tutularak fragman uzunluğu analizi gerçekleştirilmiştir. Sekiz lokustan altısı (%75) PCR ile amplifiye olmuş ve bunlardan dördü (%66.7) polimorfik bulunmuştur. Polimorfik lokusların üçü (33HDZ496, 33HDZ749 ve 33HDZ974) genetik parametrelerin hesaplanmasında kullanılmıştır. Çalışmada toplam 40 allel tespit edilmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için sırasıyla 13, 15 ve 12 allel gözlenmiş olup, herbir lokus için hesaplanan polimorfizm bilgi içeriği (PIC) aynı sırayla 0.836, 0.821 ve 0.817 olarak belirlenmiştir. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama allel sayıları sırasıyla 10.33±1.15, 10.67±2.08 ve 11.33±0.58, etkili allel sayıları ise yine aynı sıra ile 6.131±1.42, 5.823±0.23 ve 5.870±0.75 olarak bulunmuştur. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama gözlenen heterozigotluk oranları sırasıyla 0.827±0.083, 0.808±0.044 ve 0.895±0.069, beklenen heterozigotluk oranları ise yine aynı sıra ile 0.843±0.043, 0.841±0.007 ve 0.838±0.023 olarak belirlenmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için hesaplanan FIS değerleri sırasıyla 0.0164, 0.0245 ve 0.0240 olarak bulunmuştur. Tüm populasyonlar için hesaplanan FST değeri (0.009) incelenen populasyonda gözlenen genetik çeşitliliğin %99'unun bireyler arasındaki farklılıktan kaynaklandığını göstermiştir. Çalışma sonuçları incelenen ke...

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“…Due to the great phenotypic similarity, it is hypothesized that British Alpines have given rise to Canindé goats (Nunes et al, 1997). Despite to their importance within rural smallholder farming systems in the Northeast region, little is still known about the genetic relationships between these two breeds, and therefore, the objective of this study was to determine the genetic diversity and relatedness between herds of Canindé and British Alpine goats in the States of Piauí and Ceará using heterologous microsatellite loci.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and relatedness between Canindé and British Alpine goat breeds in Northeastern Brazil accessed by microsatellite markers

et al. 2017

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ABSTRACT. The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers resulted in a total number of 145 alleles, with an average of 8.5 alleles per locus. The observed and expected heterozygosities were ≥0.687 and ≥0.627, respectively, for all loci. The polymorphic information content showed that all loci were highly informative with an overall mean of 0.757. Overall F ST across all populations and loci was 18%, which was consistent with the coefficient of gene differentiation (G ST = 0.104). AMOVA revealed that 12.8% of the variation was captured between breeds. The Bayesian STRUCTURE clustering detected the maximum likelihood for a model of two genetically distinct groups, in agreement with the number of predefined studied breeds and the two-dimensional plot from the PCoA analysis. The exotic British Alpine breed and the naturalized Brazilian Canindé breed were clearly differentiated by the microsatellite markers, indicating that these two breeds have distant genetic identities, despite the phenotypic similarity.

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Genetic relationships between two homologous goat breeds from Portugal and Brazil assessed by microsatellite markers

Cited by 20 publications

References 38 publications

Ancestry analysis of locally adapted Crespa goats from southernmost Brazil

Ancestry analysis of locally adapted Crespa goats from southernmost Brazil

Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Genetic diversity and relatedness between Canindé and British Alpine goat breeds in Northeastern Brazil accessed by microsatellite markers

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