Genetic diversity and relatedness between Canindé and British Alpine goat breeds in Northeastern Brazil accessed by microsatellite markers

Abstract

ABSTRACT. The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers resulted in a total number of 145 alleles, with an average of 8.5 alleles per locus. The observed and expected heterozygosities were ≥0.687 and ≥0.627, respectively, for all loci. The polymorphic information content showed that all loci were highly informative with an overall mean of 0.757. Overall F ST across all populations and loci was 18%, which was consistent with the coefficient of gene differentiation (G ST = 0.104). AMOVA revealed that 12.8% of the variation was captured between breeds. The Bayesian STRUCTURE clustering detected the maximum likelihood for a model of two genetically distinct groups, in agreement with the number of predefined studied breeds and the two-dimensional plot from the PCoA analysis. The exotic British Alpine breed and the naturalized Brazilian Canindé breed were clearly differentiated by the microsatellite markers, indicating that these two breeds have distant genetic identities, despite the phenotypic similarity.

Genetic diversity and phylogenetic relationship of nine sheep populations based on microsatellite markers

“…Microsatellite markers belong to a class of neutral markers, which have rich polymorphism in population and are widely distributed in the genome; therefore they are suitable for genetic diversity analysis. Previous studies indicated that microsatellite markers had been well used to assess genetic diversity and population structure of sheep breeds (Di et al, 2012;Dotsev et al, 2018;Madilindi et al, 2019;Vargas et al, 2018;Laoun et al, 2020;Ullah et al, 2020) and goat breeds (Carvalho et al, 2015;Câmara et al, 2017;E et al, 2019;Liu et al, 2019). Therefore, in this study, a panel of 26 microsatellite markers was employed to evaluate the genetic diversity, population structure and phylogenetic relationship of the nine sheep populations.…”

Recursos Genéticos De Caprinos: Cenário E Sustentabilidade

“…A conservação desses recursos genéticos representa uma grande contribuição para manutenção da diversidade genética dos animais, que estão adaptados às condições ambientais do país. Apesar de sua importância, existem poucos conhecimentos sobre a formação, migração e evolução do status genético das raças brasileiras de caprinos locais, sendo, portanto, necessária uma formação mais técnica sobre a diversidade genética dos caprinos brasileiros (CÂMARA et al, 2017).…”

Genetic diversity estimation of Yunnan indigenous goat breeds using microsatellite markers

“…Currently, the abundant genetic diversity of goats has been assessed by using many different types of molecular markers, including microsatellites (Câmara, Nunes, Diniz, Silva, & Araújo, 2017;E et al, 2018), MHC region variants (Gowane, Akram, Misra, Prakash, & Kumar, 2018), mitochondrial DNA (E et al, 2018;Tarekegn et al, 2018), functional genes (Gowane et al, 2018;Pitarch et al, 2018), and genomewide SNPs (Ilie, Kusza, Sauer, & Gavojdian, 2018;Nicoloso et al, 2015;Onzima et al, 2018). Currently, the abundant genetic diversity of goats has been assessed by using many different types of molecular markers, including microsatellites (Câmara, Nunes, Diniz, Silva, & Araújo, 2017;E et al, 2018), MHC region variants (Gowane, Akram, Misra, Prakash, & Kumar, 2018), mitochondrial DNA (E et al, 2018;Tarekegn et al, 2018), functional genes (Gowane et al, 2018;Pitarch et al, 2018), and genomewide SNPs (Ilie, Kusza, Sauer, & Gavojdian, 2018;Nicoloso et al, 2015;Onzima et al, 2018).…”

“…Goats (Capra hircus) are economically important domestic animals worldwide. Currently, the abundant genetic diversity of goats has been assessed by using many different types of molecular markers, including microsatellites (Câmara, Nunes, Diniz, Silva, & Araújo, 2017;E et al, 2018), MHC region variants (Gowane, Akram, Misra, Prakash, & Kumar, 2018), mitochondrial DNA (E et al, 2018;Tarekegn et al, 2018), functional genes (Gowane et al, 2018;Pitarch et al, 2018), and genomewide SNPs (Ilie, Kusza, Sauer, & Gavojdian, 2018;Nicoloso et al, 2015;Onzima et al, 2018). The diversity of several indigenous goat breeds in China was assessed Liu et al, 2014;Wang et al, 2017).…”