Genetic relationships among resynthesized, semi-resynthesized and natural Brassica napus L. genotypes

Sosnowska, Katarzyna; Cegielska-Taras, Teresa; Liersch, Alina; Karłowski, Wojciech M.; Bocianowski, Jan; Szała, Laurencja; Mikołajczyk, Katarzyna; Popławska, W.

doi:10.1007/s10681-017-2000-7

Cited by 6 publications

(8 citation statements)

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“…Similar results were obtained by Sosnowska et al (2017) in investigations of collections of spring and WOSR cultivars, B. rapa and Brassica oleracea, as well as nearly 50 RS lines and semi-RS lines. Confirmation of the distinctiveness of resynthesized lines by AFLP markers shows that these lines can be valuable material for creating separate gene pools, especially for hybrid breeding of WOSR (Szała et al 2016;Sosnowska et al 2017). Jesske et al (2013) analyzed 126 genotypes including 55 natural B. napus genotypes (winter, spring, Asian), and vegetable/cabbage forms and 71 resynthetic lines using 20 AFLP primer combinations.…”

Section: Discussionsupporting

confidence: 85%

“…The genetic diversity of resynthesized (RS) and semi-resynthesized (semi-RS) lines has also been demonstrated in studies of 101 breeding lines and WOSR cultivars using 10 AFLP primer combinations (Liersch et al 2016). Similar results were obtained by Sosnowska et al (2017) in investigations of collections of spring and WOSR cultivars, B. rapa and Brassica oleracea, as well as nearly 50 RS lines and semi-RS lines. Confirmation of the distinctiveness of resynthesized lines by AFLP markers shows that these lines can be valuable material for creating separate gene pools, especially for hybrid breeding of WOSR (Szała et al 2016;Sosnowska et al 2017).…”

Section: Discussionsupporting

confidence: 59%

“…This approach enables the comprehensive selection of CMS ogura lines and restorer lines which will be used for further crosses and then evaluated for general and specific combination ability in multi-experimental field trials (Nowosad et al 2016(Nowosad et al , 2017Bocianowski et al 2018Bocianowski et al , 2019. In addition, demonstrating the significant genetic separateness of the resynthesized lines (RS) of rapeseed from commonly cultivated double low WOSR cultivars will enable a broadening of the variability within Brassica and the use of RS lines in breeding new winter rapeseed cultivars (Szała et al 2016;Sosnowska et al 2017).…”

Section: Discussionmentioning

confidence: 99%

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Creation of gene pools with amplified fragment length polymorphis markers for development of winter oilseed rape (Brassica napus L.) hybrid cultivars

et al. 2019

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The genetic diversity of two winter oilseed rape (Brassica napus L.) collections was investigated using amplified fragment length polymorphism marker technology. The first collection of 78 genotypes was analyzed using 11 primer-enzyme combinations and PCR products were resolved by 13.35% denaturing polyacrylamide gels. The second collection was investigated using seven fluorescent-labeled AFLP primer combinations and PCR products were separated using capillary electrophoresis. A total of 262 polymorphic AFLP markers were obtained for the first collection and 423 polymorphic markers for the second. On the basis of all markers, principal component analysis was performed for both collections separately. The unweighted pair group method with arithmetic method based on the coefficient of dissimilarity separated the genotypes in collection 1 into two clusters and those in the second collection into three distinct clusters. Resynthesized lines formed a cluster that was clearly distinct from 42 winter oilseed rape parental lines in the cytoplasmic male sterility ogura system. Analysis of molecular variance showed that 79% and 81% of the detected variation was found to be within the groups (in the two collections, respectively), while the variation between groups contributed, respectively, to only 21% and 19% of the variance. Our results indicate that AFLP technology can be useful for the creation of a gene pool of parental components of winter oilseed rape hybrid cultivars. Additionally, de novo resynthesized Brassica napus lines provide a significant opportunity for enrichment of the gene pool of winter rapeseed.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

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Creation of gene pools with amplified fragment length polymorphis markers for development of winter oilseed rape (Brassica napus L.) hybrid cultivars

et al. 2019

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“…Due to their wide adaptation and ability to thrive under varying agroclimatic conditions, brassica crops are grown throughout the world for food, animal forage, fodder and industrial applications (Eckes et al 2017). In Poland within Brassica species, winter oilseed rape is the most important oil-protein plant (Liersch et al 2016;Szała et al 2016;Sosnowska et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Genotype-by-environment interaction for seed quality traits in interspecific cross-derived Brassica lines using additive main effects and multiplicative interaction model

2018

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The aim of this study was to assess genotype by environment interaction for seed quality traits in interspecific cross-derived Brassica lines by the additive main effects and multiplicative interaction (AMMI) model. The study comprised of 25 winter rapeseed genotypes i.e.: B. napus cultivar Californium, twenty three cross-derived Brassica lines and male sterile line of an F 8 generation of B. napus (MS8), selected from resynthesized oilseed rape (B. rapa ssp. chinensis 9 B. oleracea var. gemmifera) using in vitro cultures of isolated embryos. Field trials were performed at three locations in 3 years in a randomized complete block design, with three replicates. AMMI analyses revealed significant genotype and environmental effects as well as genotype by environmental interaction with respect to all five observed traits. The lines 16 (B. napus line MS8 9 B. rapa ssp. pekinensis) and 7 (B. napus line MS8 9 B. carinata) are recommended for further inclusion in the breeding programs because their stability and good average values of observed traits, except total glucosinolates content for line 16 (the best total genotype selection indexes were equal to 81 and 97, respectively).

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“…Resynthesized oilseed rape was obtained as a result of crosses between B. rapa and B. oleracea using two methods: in vivo pollination (Sosnowska et al 2010 ) and in vitro placental pollination (Sosnowska and Cegielska-Taras 2014 ) and then through in vitro culture of isolated embryos in the early stage of their development (embryo rescue culture). Molecular analysis using AFLP markers and 10 primer combinations performed in our previous studies showed a high degree of diversity among one hundred Brassica genotypes, including de novo synthesized oilseed rape lines (Sosnowska et al 2017 ). Natural accessions of B. napus generate a compact group, which is distinct from RS and semi-RS lines.…”

Section: Introductionmentioning

confidence: 97%

Chromosome instabilities in resynthesized Brassica napus revealed by FISH

Sosnowska

Majka

et al. 2020

J Appl Genetics

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Brassica napus is an allopolyploid plant, derived from spontaneous hybridization between Brassica rapa and Brassica oleracea. Intensive breeding has led to a significant reduction in genetic and phenotypic diversity within this species. Newly resynthesized hybrids from progenitor species may restore some diversity in B. napus, but they often are chromosomally and phenotypically unstable. Using fluorescence in situ hybridization, we tested chromosome constitutions in a range of new allopolyploids resynthesized from various parental species. A majority of these allopolyploids were euploid, with the expected chromosome numbers and constitutions, but deviations were also identified. We detected a low level of intergenomic rearrangements in analyzed hybrids and a high level of changes in rDNA loci. Our study revealed a significant effect of maternal cross combination on loss of 35S rDNA loci, especially when B. rapa was the maternal parent. The studied lines were characterized by diversified of pollen viability. In the analyzed hybrids, the erucic acid level in the seed oil ranged from 0 to 43.4% and total glucosinolate content in seeds ranged from 24.3 to 119.2 μmol g −1. Our study shows that cytogenetic analysis of B. napus resynthesized hybrids would be useful in breeding for the selection of lines with important agricultural characters and genetically stable stock seed production. Keywords Allopolyploids. Resynthesis. Brassica napus. Chromosome rearrangements. FISH. rDNA Abbreviations BAC Bacterial artificial chromosome FISH Fluorescence in situ hybridization NOR Nucleolus organizer region RS Resynthesized 00-quality Genotypes with zero erucic acid and low seed glucosinolate content 0+ quality Genotypes with zero erucic acid and high glucosinolate content ++ quality Genotypes with high erucic acid and high glucosinolate content * Katarzyna Sosnowska

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Genetic relationships among resynthesized, semi-resynthesized and natural Brassica napus L. genotypes

Cited by 6 publications

References 30 publications

Creation of gene pools with amplified fragment length polymorphis markers for development of winter oilseed rape (Brassica napus L.) hybrid cultivars

Creation of gene pools with amplified fragment length polymorphis markers for development of winter oilseed rape (Brassica napus L.) hybrid cultivars

Genotype-by-environment interaction for seed quality traits in interspecific cross-derived Brassica lines using additive main effects and multiplicative interaction model

Chromosome instabilities in resynthesized Brassica napus revealed by FISH

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