Genetic Relationships Among <I>Aedes aegypti</I> (Diptera: Culicidae) Populations from Argentina Using Random Amplified Polymorphic DNA Polymerase Chain Reaction Markers

Genetic structure of Aedes aegypti in the city of Córdoba (Argentina), a recently reinfested area

“…aegypti from Córdoba showed high levels of genetic variability, as revealed by the analysis of 50 RAPD loci. Values of expected H were similar to those found previously by de Sousa et al (2001) in several Argentine cities using only 17 RAPD loci. Likewise, levels of variability were similar to those found in other populations from Brazil (Ayres et al 2003, Paduan et al 2006), Puerto Rico (Apostol et al 1996) and Mexico (Gorrochotegui-Escalante et al 2000), using the same molecular markers.…”

“…A total of 50 RAPD bands, ranging in size from 448-1,769 bp, were selected for analyses, 17 of which were the same fragments used by de Sousa et al (2001) to analyse samples from different cities in Argentina. Examples of the amplification patterns obtained with the new RAPD primers are shown in Fig.…”

“…PCR were carried out as described by de Sousa et al (2001). In each reaction, a negative control (including ddH 2 O instead of mosquito DNA) was included to test for contamination with foreign DNA.…”

“…The same arbitrary primers analyzed by de Sousa et al (2001) were used for PCR amplifications: OP-A02 (5' -TGCCGAGCTG-3'), OP-B03 (5'-ACTTCGACAA-3') and OP-B13 (5'-TTCCCCCGCT-3') from Operon Technologies (Alameda, CA). The same 17 bands used by de Sousa et al (2001) to analyze the genetic differentiation among Ae.…”

“…The same 17 bands used by de Sousa et al (2001) to analyze the genetic differentiation among Ae. aegypti from five cities in Argentina were screened.…”