Genetic structure of Aedes aegypti in the city of Córdoba (Argentina), a recently reinfested area

Julio, Norma; Chiappero, Marina B.; Rossi, Hernán J; Dueñas, Juan C. Rondan; Gardenal, Cristina N.

doi:10.1590/s0074-02762009000400016

Cited by 19 publications

(9 citation statements)

References 33 publications

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“…This agrees with the results of other genetic analyses of populations corresponding to several vector insects (mainly mosquitoes), where their reproducibility and polymorphism have also been shown (de Sousa et al, 1999;Gorrochotegui-Escalante et al, 2000;Fonseca et al, 2001;dos Santos et al, 2003;Julio et al, 2009). For instance, Ayres et al (2002), in study done on genetic diversity in 6 Brazilian Aedes albopictus populations, obtained just reproducibility and efficiency in 4 of the 10 primers tested.…”

Section: Discussionsupporting

confidence: 91%

“…The same species studied in populations from Puerto Rico had an average value of 0.354 (Apostol et al, 1996). Identically, Julio et al (2009) estimated similar levels of genetic variability (0.351-0.404) in population samples of this mosquito from different neighborhoods in Cór-doba City, Argentina. In addition, Posso et al (2003) recorded an average value of 0.340 in the study of 3 Colombian Anopheles nuñeztovari populations.…”

Section: Discussionmentioning

confidence: 68%

“…RAPD-polymerase chain reaction (PCR) is a technique that generates markers by the amplification of random DNA segments with single primers of arbitrary nucleotide sequence (Williams et al, 1990). These molecular markers have been successfully used in genetic variability studies; they were applied to natural populations of different mosquito species including Aedes albifasciatus (de Sousa et al, 1999), Aedes (Finlaya) japonicus (Fonseca et al, 2001), Aedes albopictus (Ayres et al, 2002), Anopheles nuñeztovari (Posso et al, 2003), Anopheles superpictus and Anopheles pulcherrimus (Abramova et al, 2005), Anopheles darlingi (González et al, 2007), and Aedes aegypti (Julio et al, 2009). However, there are no reports of studies performed on the genetics of O. taeniorhynchus populations on the Colombian Atlantic coast using these types of markers.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers

2014

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ABSTRACT. Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemicepizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito's genetic variability and genetic structure of 122 specimens in 7 populations from the Colombian Atlantic coast. Assuming that all loci were in Hardy-Weinberg equilibrium, diversity statistics and analyses were performed. The average number of amplified fragments for each primer was 8.3, and the size of these fragments ranged from 350 to 3600 bp. The expected average heterozygosity was 0.358 ± 0.103. The genetic heterogeneity among the populations studied was small (G ST = Genetic analysis of Ochlerotatus taeniorhynchus 0.05 ± 0.01); meanwhile, the gene flow estimates (N m = 7.32 ± 1.35) were high. In an identical way, the Nei's genetic distances obtained yielded very small values amongst the populations that were studied in this Colombian region. Furthermore, a spatial autocorrelation analysis with Moran's I index revealed a very weak, or inexistent, spatial genetic structure among these populations. The comparison of these results with those performed for other markers (isoenzymes and microsatellites) in populations of this same mosquito from the Colombian Atlantic coast was discussed. The results of our RAPD analysis showed scarce genetic differentiation among the mosquito populations on the Colombian Atlantic coast, which was probably determined by high gene flow levels.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

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Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers

2014

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“…Aun bajo esta consideración, los índices son empleados junto a otros factores ecosistémicos que amplían la información disponible y que, en cierta medida, explicaría el origen de estos índices. La evidencia de muchas investigaciones a nivel mundial, relacionan tres variables principales a la alta incidencia histórica de dengue y a valores elevados de infestación local de A. aegypti: la presión de vapor (una variable que combina la humedad y la temperatura), la densidad poblacional humana, y la disponibilidad en el medio ambiente de recipientes que acumulen agua 49,51,52 . A los fines del control vectorial, la magnitud de la actividad de dispersión de A. aegypti aún no está claramente definida, en tanto se reportan distancias de entre 100-800 metros -un rango de mucha variación-en Brasil 16 , mientras otros autores muestran datos entre 10 y 500 metros 53,54 .…”

Section: Discussionunclassified

Dinámica y caracterización epidemiológica del brote de dengue en Argentina año 2016: el caso de la Provincia de Buenos Aires

Bolzán

Insúa²,

Pamparana

et al. 2019

Rev. chil. infectol.

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“…García-Franco et al, 2002), Argentina and Uruguay (de Sousa et al, 2001;Julio et al, 2009;Soliani et al, 2010), and Puerto Rico (Apostol et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Genetic structure of Aedes aegypti populations determined using pairwise comparisons

Patarro¹,

Guirado²,

Ravazzi³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six primers. Four populations were from Brazil and one was from the USA. A total of 165 polymorphic DNA loci were generated. Considering the six primers and the five populations, the mean value of inter-population genetic diversity (Gst) was 0.277, which is considered high according to the Wright classification. However, pairwise comparisons of the populations gave variable Gst values ranging from 0.044 to 0.289. This variation followed the population's geographic distance to some extent but was also influenced by human activity. The lowest Gst values were obtained in the comparison of populations from cities with intensive commercial and medical contacts. These mosquito populations were previously classified as insecticide resistant, susceptible, or with decreased susceptibility; this parameter apparently had an effect on the Gst values obtained in the pairwise comparisons.

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Genetic structure of Aedes aegypti in the city of Córdoba (Argentina), a recently reinfested area

Cited by 19 publications

References 33 publications

Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers

Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers

Dinámica y caracterización epidemiológica del brote de dengue en Argentina año 2016: el caso de la Provincia de Buenos Aires

Genetic structure of Aedes aegypti populations determined using pairwise comparisons

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