Dinámica y caracterización epidemiológica del brote de dengue en Argentina año 2016: el caso de la Provincia de Buenos Aires

Bolzán, Andrés; Insúa, Iván; Pamparana, Carolina; Giner, María Celeste; Medina, Ana María Robles; Zucchino, Betina

doi:10.4067/s0716-10182019000100016

Cited by 6 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AMBA has a more recent history of dengue outbreaks, which represented 35% of the most important dengue outbreaks registered in Argentine in 2016 [ 35 ], 2020 and 2023 (Bulletin of the Ministry of Health Argentina), despite it being the most populated region of Argentina. Interestingly, a positive correlation exists between the specific years of treatment with pyrethroids and the emergence of the 1016I kdr mutation.…”

Section: Discussionmentioning

confidence: 99%

First report on knockdown resistance mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high-resolution melting polymerase chain reaction method

Barrera-Illanes,

Micieli,

Ibáñez-Shimabukuro

et al. 2023

Parasites Vectors

View full text Add to dashboard Cite

Background The mosquito Aedes aegypti is an urban vector of dengue and other arboviruses. During epidemics of these viruses, pyrethroid insecticides are used for the control of adult mosquitoes. The worldwide resistance of Ae. aegypti to these insecticides is a cause of failure of vector control campaigns. The primary target of pyrethroids is the voltage-gated sodium channel. Point mutations in the gene coding for this channel, called knockdown resistance (kdr) mutations, are associated with pyrethroid resistance. Two kdr mutations, V1016I and F1534C, have increased in frequency in natural populations of Ae. aegypti in the Americas during the last decade. Their association with pyrethroid resistance has been largely demonstrated in field populations throughout the Americas, and in in vitro assays. Diagnostics for kdr polymorphism allow early detection of the spread of insecticide resistance, which is critical for timely decisions on vector management. Given the importance of resistance management, high-throughput methods for kdr genotyping are valuable tools as they can be used for resistance monitoring programs. These methods should be cost-effective, to allow regional-scale surveys. Despite the extensive presence of Ae. aegypti and incidence of dengue in Argentina, the presence, abundance, and distribution of kdr mutations in populations of this mosquito have yet to be reported for the country. Methods Aedes aegypti samples were collected as immature stages or adults from Buenos Aires Metropolitan Area and northern localities of Tartagal (Salta Province) and Calilegua (Jujuy Province). Immature stages were maintained in the laboratory until they developed into adults. A high-resolution melting assay, based on an analysis of melting temperatures, was developed for the simultaneous genotyping of V1016I and F1534C kdr mutations. We used this method to infer the presence and frequencies of kdr alleles in 11 wild populations from Argentina. Results We demonstrated the presence of kdr mutations in Ae. aegypti in Argentina in regions where this species is under different selection pressures due to the use of pyrethroids. The populations under analysis are located in geographically distant regions of the species’ distribution in Argentina: the northern provinces of Salta and Jujuy and the Buenos Aires Metropolitan Area. Higher frequencies of resistant-associated alleles were detected in the northern region. We report a multiplex high-throughput assay based on a high-resolution melting polymerase chain reaction method for the simultaneous genotyping of V1016I and F1534C kdr mutations. This assay was shown to be cost-effective, and thus provides an interesting molecular tool for kdr genotyping in A. aegypti control campaigns. Conclusions We report, to the best of our knowledge for the first time, the presence of kdr mutations in populations of Ae. aegypti from geographically distant locations of Argentina that differ with respect to their epidemiological situation and history of mosquito control. We have developed a high-throughput method for the genotyping of kdr mutations in Ae. aegypti from the Americas. Given its affordability and short running time, this method can be used in control campaigns to monitor the presence and spread of kdr alleles. The information provided here is relevant for the rational design of control strategies in the context of integrated vector management. Graphical Abstract

show abstract

Section: Discussionmentioning

confidence: 99%

First report on knockdown resistance mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high-resolution melting polymerase chain reaction method

Barrera-Illanes,

Micieli,

Ibáñez-Shimabukuro

et al. 2023

Parasites Vectors

View full text Add to dashboard Cite

show abstract

“…Buenos Aires Metropolitan Area has a more recent history of dengue outbreaks, despite being the most populated region from Argentina, representing 35% of the total population with two important dengue outbreaks registered in 2016 [32] and 2020 (Bulletin from the Ministry of Health Argentina. 2020).…”

Section: Discussionmentioning

confidence: 99%

First report on kdr mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high resolution melting PCR method

Illanes

Micieli

Shimabukuro

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Aedes aegypti is an urban mosquito vector of dengue and other arboviruses. During epidemic periods, pyrethroid insecticides are used for the control of adult mosquitoes; the worldwide distributed resistance to these insecticides is a cause of failures in vector control campaigns. The primary target of pyrethroids is the voltage-gated sodium channel; point mutations on this channel, called kdr mutations, are associated with pyrethroid resistance. Two kdr mutations, called V1016I and F1534C, augmented in frequency in natural populations of Ae. aegypti from the Americas in the last decade; their association with pyrethroid resistance has been largely demonstrated in field populations throughout the country, and in in vitro assays. The diagnostic of kdr polymorphisms allows an early detection of insecticide resistance spreading, which is critical for timely decisions on vector management. Given the relevance of resistance management, high-throughput methods for kdr genotyping are valuable tools for resistance monitoring programs. These methods should also be cost-effective, to allow regional-scale surveys. Despite the extended presence of Ae. aegypti and the incidence of dengue in Argentina, the presence, abundance and distribution of kdr mutations were not reported in this country up to date. Methods: Aedes aegypti samples were collected from Metropolitan Area of Buenos Aires, and northern localities of Tartagal (Salta province) and Calilegua (Jujuy province). A high resolution melting assay was developed for the simultaneous detection of V1016I and F1534C kdr mutations. Results: We demonstrate the presence of kdrmutations in Argentina in regions under different selection pressure with pyrethroids. We also report a multiplex high-throughput assay based in high resolution melting PCR for the simultaneous genotyping of V1016I and F1534C kdr mutations. Conclusions: We report for the first time the presence of kdr mutations in distant populations from Argentina, with different epidemiological situations and different history of mosquito control efforts. We have developed a high-throughput method for the genotyping of kdr mutations in Ae. aegypti from the American continent. Given its convenient cost and running time, this method can be incorporated in control campaigns for monitoring the presence and spreading of kdr alleles.

show abstract

“…aegypti adults from Argentina, in Salta Province (Salvador Mazza). Buenos Aires Metropolitan Area has a more recent history of dengue outbreaks, in despite of being the most populated region from Argentina, representing 35% of the total population with two important dengue outbreaks registered in 2016 [33] and 2020 (Bulletin from the Ministry of Health Argentina. 2020).…”

Section: Discussionmentioning

confidence: 99%

Multiplex high resolution melting PCR for simultaneous genotyping of pyrethroid-resistance associated mutations in Aedes aegypti. First report on kdr mutations in wild populations from Argentina

Illanes

Micieli

Shimabukuro

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Aedes aegypti is an urban mosquito vector of Dengue and other arboviruses. During epidemic periods, pyrethroid insecticides are used for the control of adult mosquitoes; the worldwide distributed resistance to these insecticides is a cause of failures in vector control campaigns. The primary target of pyrethroids is the voltage-gated sodium channel; point mutations on this channel, called kdr mutations, are associated with pyrethroid resistance. Two kdr mutations, called V1016I and F1534C, augmented in frequency in natural populations of Ae. aegypti from the Americas in the last decade. The diagnostic of kdr polymorphisms allows an early detection of insecticide resistance spreading, which is critical for timely decisions on vector management. Given the relevance of resistance management, high-throughput methods for kdr genotyping are invaluable tools for resistance monitoring programs. These methods should also be cost-effective, to allow regional-scale surveys. Despite the extended presence of Ae. aegypti and the incidence of dengue in Argentina, the presence, abundance and distribution of kdr mutations were not reported in this country up to date. Methodology and findings: We report a multiplex high-throughput assay based in High Resolution Melting PCR for the simultaneous genotyping of 1016 and 1534 sites in voltage-gated sodium channel gene. We used this method for the study of individual mosquito samples collected in localities which received different selection pressure with pyrethroids. Compared to other genotyping methods, multiplex High Resolution Melting was high-throughput, cost-efficient, sensitive and specific. We demonstrate for the first time the presence of kdr mutations in Argentina in regions under different selection pressure with pyrethroids. Conclusions and Significance: We have developed a high-throughput method for the genotyping of alleles associated with pyrethroid resistance in Ae. aegypti from the American continent. The method developed here is comparable in its sensitivity and reliability with other genotyping methods, but reduces costs and running time. It could be incorporated in control campaigns for control the presence and spreading of resistance-associated alleles. We report here for the first time the presence of kdr mutations in distant populations from Argentina, with different epidemiological situations and different history of mosquito control efforts.

show abstract

Dinámica y caracterización epidemiológica del brote de dengue en Argentina año 2016: el caso de la Provincia de Buenos Aires

Abstract: El presente trabajo no registra conflicto de intereses. No tuvo aportes específicos para la realización del estudio.

Cited by 6 publications

References 22 publications

First report on knockdown resistance mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high-resolution melting polymerase chain reaction method

First report on knockdown resistance mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high-resolution melting polymerase chain reaction method

First report on kdr mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high resolution melting PCR method

Multiplex high resolution melting PCR for simultaneous genotyping of pyrethroid-resistance associated mutations in Aedes aegypti. First report on kdr mutations in wild populations from Argentina

Contact Info

Product

Resources

About