Genetic Polymorphism G894T and the Prognosis of Heart Failure Outpatients

Abstract

BackgroundPrevious studies have analyzed the role of the genetic polymorphism of endothelial nitric oxide synthase on heart failure prognosis. However, there are no studies relating the G894T and heart failure in Brazil. ObjectiveTo evaluate the association between G894T GP and the prognosis of a sample of Brazilian outpatients with heart failure. MethodsCohort study included 145 patients with systolic heart failure, followed for up to 40 months (mean = 22), at two university hospitals, in the State of Rio de Janeiro. We evaluated the relationship between G894T and the following outcomes: reverse remodeling, improvement in functional class (NYHA), and mortality and hospitalization rates. The diameters of the left atrium and ventricle, as well as the ejection fraction of the left ventricle, were evaluated at baseline and at 6 months to assess reverse remodeling. The improvement in functional class was evaluated after 6 months, and mortality rate and hospitalization were evaluated during follow-up. Race was self-declared. G894T polymorphism was analyzed by polymerase chain reaction and restriction fragment length polymorphism. ResultsThe genotypic frequencies were GG (40%), GT (48.3%) and TT (11.7%). The allele frequency was guanine (64.1%) and thiamine (35.8%). There were no differences between the genotype or allelic frequencies according to self declared race, either as baseline characteristics. There was no relationship between genotype or allele frequency and the outcome measures. ConclusionNo association was observed between the G894T polymorphism (Glu298Asp) and prognosis in this sample of Brazilian outpatients with systolic heart failure.

Endothelial Nitric Oxide Synthase Single Nucleotide Polymorphism and Left Ventricular Function in Early Chronic Kidney Disease

“…For instance Velloso et al studied a multi-ethnic Brazilian population and demonstrated increased frequency of GG genotype in patients with systolic heart failure compared with healthy controls [ 7 ]. Another Brazilian study showed GG genotype was associated with a near 5% reduction in LVEF compared with TT genotype patients, findings very similar to those of the current study [ 18 ]. Also noteworthy is the higher all-cause mortality associated with the GG genotype in hypertensive patients [ 19 ].…”

The multifaceted role of genetic polymorphisms in atherosclerosis

“…G894T carriers show less NO production and it may be a factor that increases patients' susceptibility to atherosclerotic disease. The frequency of the mutant allele was more frequent in the Brazilian [62], Mexican [63] and Italian [64] populations. The wild type allele is more frequent in England [54], Germany [65], Turkey [66], South Africa [67] and in the African-American population [68].…”