Genetic Polymorphism at the Leptin Gene in Iranian Holstein Cattle by PCR-RFLP

Sharifzadeh, Ali; Doosti, Abbas; Moshkelani, S.

doi:10.3923/javaa.2010.1420.1422

Cited by 9 publications

(9 citation statements)

References 11 publications

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“…The Holstein Friesian cows investigated here were found to be polymorphic with regard to LEP‐Sau3A I polymorphism: the AA genotype was the most common, and the BB genotype was the least common genotype. These findings comply with the results of earlier studies carried out with Holstein cows in Iran (Sharifzadeh and Doosti ), Greece (Oikonomou et al . ), Jordan (Jawasreh et al .…”

Section: Discussionsupporting

confidence: 93%

Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows

Kiyici

Arslan

Akyüz

et al. 2018

Int J of Dairy Tech

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This study was conducted to identify gene polymorphisms and to investigate the relationships of polymorphisms with yield and composition of milk from Holstein cows. Significant relationships of growth hormone (GH), leptin (LEP) and myogenic factor 5 (MYF5) gene polymorphisms with 305‐day milk yield were observed (P < 0.01). Signal transducers and activators of transcription 5A (STAT5A) gene polymorphism did not show any significant relationships with investigated traits (P > 0.05). With regard to the 305‐day milk yield, GH‐LV, LEP‐AA and AB and MYF5‐GG genotypes were found to be superior over the other genotypes. It was concluded based on present findings that GH, LEP and MYF5 genes could be used as candidate genes for milk yield of Holstein cows.

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Section: Discussionsupporting

confidence: 93%

Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows

Kiyici

Arslan

Akyüz

et al. 2018

Int J of Dairy Tech

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“…Particularly in animal breeding, this technology would support to better explore genetic potential of livestock because of its speed and accuracy. Leptin gene is one of the major genes having an economic value that can be potentially used as a genetic marker (MAS; Marker Assisted Selection) to directly select a breeding stock (Sharifzadeh and Doosti, 2010).…”

Section: Introductionmentioning

confidence: 99%

Leptin gene polymorphism of Ongole Grade cattle based on single nucleotide polymorphism

Hilmia¹,

Rahmat²,

Dudi³

2018

J. Indonesian Trop. Anim. Agric.

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Point mutation on exon 2 of leptin gene, which changes amino acid encoding from Arginine to Cysteine, may alters the physiological function of the leptin hormone. This study aimed to identify leptin gene polymorphism of Ongole Grade (OG) cattle based on Single Nucleotide Polymorphism (SNP). The DNA sample was taken from 48 head of OG cattle at Balai Pengembangan Perbibitan Ternak Sapi Potong(BPPT SP) Cijeungjing West Java, which was isolated from white blood cell using the high salt method. Amplification of DNA was done by Polymerase Chain Reaction (PCR), followed by direct sequencing to obtain nucleotide sequence. The SNP analysis was carried out from alignment of sequencing result using Bioedit and MEGA 5.2 program. The results indicated in exon 2 leptin gene of OG cattle there was one synonymous SNPs that did not changeamino acids Serine encoding on g.1025T >C/S17S, while two non synonymous SNPaltered amino acids encoding, those were g.1047C> T /R25C and g.1048G>A/R25H. Those mutations changed amino acids encoding from Arginine to Cysteine and Arginine to Histidine respectively.In OG cattle, the frequency of A allele (44.8%) was higher than C allele (33.3%) and T allele (21.9%). Six genotypes were also identified, i.e. AA (41.7%), CC (20.8%), CT (20.8%), CA(4.2%), TT (10.4%) and TA (2.1 %). Heterozigosity of OG cattle based on leptin gene was 0.65 that was a high category. The A allele was a specific allele on Indonesian local cattle.

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“…In another study examining LEP-Sau3AI polymorphism in 112 Holstein cattle raised in Iran, A allele frequency and B allele frequency were 0.798 and 0.202 respectively. In the same study, the frequencies of the AA genotype, AB genotype and BB genotypes were 0.607, 0.38 and 0.202, respectively (39). In a study conducted on Holsteins bred in Greece, the frequency of the AA and AB genotypes were 0.78 and 0.22, respectively, while the BB genotype was not detected.…”

Section: Discussionmentioning

confidence: 81%

“…In previous studies, AA genotype was reported to have a negative effect on milk yield (21,28) and live weight gain (1) while BB genotype was associated with improved body condition score. Upon consideration of the results of prior studies (15,39,44) in addition to this one, we speculate that breeders artificially increased B allele frequency through their selection of female calves with high milk yield. This study and similar studies of LEP-Sau3AI polymorphism in Holstein cattle bred in different countries show that AA genotype is the most common.…”

Section: Discussionmentioning

confidence: 88%

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Bayram¹,

Arslan²,

Akyüz³

et al. 2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X 2 :40.75, p<0.001and X 2 :8.4, p<0.01). Türkiye'de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü-1 (PIT-1) ve leptin (LEP) gen polimorfizmlerinin belirlenmesiÖzet: Bu çalışmada Türkiye'de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç'lik ve LEP geni için 422 bç'lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X 2 :40.75, p<0.001ve X 2 :8.4, p<0.01).

show abstract

Genetic Polymorphism at the Leptin Gene in Iranian Holstein Cattle by PCR-RFLP

Cited by 9 publications

References 11 publications

Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows

Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows

Leptin gene polymorphism of Ongole Grade cattle based on single nucleotide polymorphism

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

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