Genetic insights unraveling quorum quenching potential of indigenous isolates from an anaerobic membrane bioreactor

Waheed, Hira; Mehmood, Ch. Tahir; Li, Yiwei; Yang, Yongyu; Xiao, Yeyuan

doi:10.1016/j.scitotenv.2021.152349

Cited by 14 publications

(17 citation statements)

References 31 publications

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“…YS11 (0.013/hour) compared to that of B. agri (0.007/hour). These results contrasted with a previous study in which the highest OD 600 of Lysinibacillus was 0.4 (Waheed et al, 2022). With the addition of C 6 -HSL and C 8 -HSL, P. aeruginosa was observed in the highest OD 600 of 0.98.…”

Section: Growth Characteristics Of Isolated Qq Strainscontrasting

confidence: 99%

Isolation and characterization of quorum quenching bacteria from municipal solid waste and bottom ash co-disposal landfills

et al. 2023

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Co-landfilling of bottom ash (BA) accelerates the clogging of leachate collection systems (LCSs) and increases the risk of landfill failure. The clogging was mainly associated with bio-clogging, which may be reduced by quorum quenching (QQ) strategies. This communication reports on a study of how isolated facultative QQ bacterial strains from municipal solid waste (MSW) landfills and BA co-disposal landfills. In MSW landfills, two novel QQ strains ( Brevibacillus agri and Lysinibacillus sp. YS11) can degrade the signal molecule hexanoyl-l-homoserine lactone (C6–HSL) and octanoyl-l-homoserine lactone (C8–HSL), respectively. Pseudomonas aeruginosa could degrade C6–HSL and C8–HSL in BA co-disposal landfills. Moreover, P. aeruginosa (0.98) was observed with a higher growth rate (OD600) compared to that of B. agri (0.27) and Lysinibacillus sp. YS11 (0.53). These results indicated that the QQ bacterial strains were associated with leachate characteristics and signal molecules and could be used for controlling bio-clogging in landfills.

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Section: Growth Characteristics Of Isolated Qq Strainscontrasting

confidence: 99%

Isolation and characterization of quorum quenching bacteria from municipal solid waste and bottom ash co-disposal landfills

et al. 2023

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“…Recently, the whole genomes of AnQ-m1 has been sequenced and annotated; amidase was determined to be the only QQ enzyme encoded by its genome. 36 Encapsulation of QQ isolates in modified alginate beads has been the most successful application among all QQ-based biofouling control methods. Previously, we coated alginate beads with polysulfone materials, which showed excellent stability and biofouling control efficiency in aerobic MBRs.…”

Section: Resultsmentioning

confidence: 99%

“…This significantly higher concentration of AHLs necessitates a longer degradation time. Recently, the whole genomes of AnQ-m1 has been sequenced and annotated; amidase was determined to be the only QQ enzyme encoded by its genome …”

Section: Resultsmentioning

confidence: 99%

Biofouling Control in an Anaerobic Membrane Bioreactor with Indigenous Quorum Quenching Bacterium Micrococcus luteus Strain AnQ-m1

Xiao

Waheed

et al. 2022

ACS EST Water

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This study isolated and screened indigenous strains quenching the acyl homoserine lactone (AHL)-mediated quorum sensing system and investigated the effectiveness of a selected strain for biofouling control in a laboratory-scale anaerobic membrane bioreactor (AnMBR). Among the 11 facultative anaerobes isolated from anaerobic sludge, Micrococcus luteus strain AnQ-m1 was screened out as the most potent AHL degrader. Its AHL degradability was further examined while encapsulating the cells in polysulfone−alginate beads to optimize the encapsulation medium recipe. The QQ beads with immobilized AnQ-m1 cells retarded the apparent membrane fouling rate by 2.2 times compared with the AnMBR with vacant beads. C8-HSL, the dominant AHL in AnMBRs, was effectively (∼70%) quenched in the QQ-AnMBR before the membrane was fouled; however, its concentration was increased to the normal level when the membrane was fouled. Polysaccharides in a loosely bound extracellular polymeric substance were gradually reduced at a constant rate during the QQ stage, while the indigenous QQ strain barely affected the microbial community structure of the bulk sludge and the water treatment performance of the AnMBR. These findings provide new insights into the QQ-based biofouling control strategy and highlight the beneficial effects of indigenous QQ strains on AnMBR stability.

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“…Although these enzymes do not directly hydrolyze AHLs, this modification process reshapes the structure of AHL molecules, thus affecting their binding with cognate receptors and blocking the QS system. However, there are few reports about AHL oxidoreductases, and most of them are limited to the discovery of new enzymes such as CYP102A5 (Waheed et al., 2022) and NADH‐dependent enzyme BpiB09 (Bijtenhoorn et al., 2011), whose overexpression reduced the QS‐regulated pathogenicity of P. aeruginosa . Therefore, despite AHL oxidoreductase showing a unique quenching mode for AHLs, it is not a current focus of QQ enzyme‐related research.…”

Section: The Opportunity Of Qq Enzymes In the Food And Related Indust...mentioning

confidence: 99%

Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

Dangfeng

Cui

Ren

et al. 2023

Comp Rev Food Sci Food Safe

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The problems of spoilage, disease, and biofilm caused by bacterial quorum‐sensing (QS) systems have posed a significant challenge to the development of the food industry. Quorum‐quenching (QQ) enzymes can block QS by hydrolyzing or modifying the signal molecule, making these enzymes promising new candidates for use as antimicrobials. With many recent studies of QQ enzymes and their potential to target foodborne bacteria, an updated and systematic review is necessary. Thus, the goals of this review were to summarize what is known about the effects of bacterial QS on the food industry; discuss the current understanding of the catalytic mechanisms of QQ enzymes, including lactonase, acylase, and oxidoreductase; and describe strategies for the engineering and evolution of QQ enzymes for practical use. In particular, this review focuses on the latest progress in the application of QQ enzymes in the field of food. Finally, the current challenges limiting the systematic application of QQ enzymes in the food industry are discussed to help guide the future development of these important enzymes.

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Genetic insights unraveling quorum quenching potential of indigenous isolates from an anaerobic membrane bioreactor

Cited by 14 publications

References 31 publications

Isolation and characterization of quorum quenching bacteria from municipal solid waste and bottom ash co-disposal landfills

Isolation and characterization of quorum quenching bacteria from municipal solid waste and bottom ash co-disposal landfills

Biofouling Control in an Anaerobic Membrane Bioreactor with Indigenous Quorum Quenching Bacterium Micrococcus luteus Strain AnQ-m1

Quorum‐quenching enzymes: Promising bioresources and their opportunities and challenges as alternative bacteriostatic agents in food industry

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