Genetic fingerprinting and phylogenetic diversity of Staphylococcus aureus isolates from Nigeria

Onasanya, A.; Mignouna, H.D.; Thottappilly, G.

doi:10.5897/ajb2003.000-1051

Cited by 27 publications

(27 citation statements)

References 18 publications

(17 reference statements)

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“…Pathogenic strains are usually coagulase-positive [6] and have been found to cause disease in their hosts throughout the world [7,8]. Diseases in cattle caused by Staphylococcus aureus range from simple abscesses and mastitis to the more severe toxic shock syndrome [7-9]. Milk is an excellent growth medium for a large number of micro-organisms, including S. aureus [10].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia

Daka

Solomon

silassie

et al. 2012

Ann Clin Microbiol Antimicrob

115

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BackgroundQuarter milk samples from cows were examined to determine the prevalence of Staphylococcus aureus (SA) and different antibiotic resistant pattern were determined in a cross-sectional study design.ObjectiveThe objective of this study was to isolate Staphylococcus aureus from samples of cow’s milk obtained from Hawassa area and to determine their antibiotic susceptibility patterns.MethodA total of 160 milk (CCP1-CCP5) samples were collected and screened for the presence of S. aureus. Gram staining, oxidase, catalase, DNase, haemolysis and coagulase tests were employed for bacterial identification.ResultsAll the samples were contaminated with S. aureus. A total of 78 S. aureus isolates were obtained during this study. The levels of contamination with S. aureus were higher in milk obtained from CCP1, CCP2, CCP3, CCP4 and CCP5 at Hawassa area farms (18.0%, 25.6%, 27.0%, 21.8% and 7.7%) respectively. A large percentage of the S. aureus isolates (25.6% and 27.0%) were from CCP2 and CCP3. All strains were resistant to Penicillin G (PG) (10 μg), Ampicillin (AP) (10 μg), Amoxicillin-Clavulanic acid (AC) (30 μg), Ciprofloxacin (CIP) (5 μg), Erythromycin (E) (15 μg), Ceftriaxone (CRO) (30 μg), Trimethoprime-Sulfamethoxazole (TMP-SMZ) (25 μg) Oxacillin (Ox) (1 μg) and Vancomycin (V) (30 μg), 67.9%, 70.9%, 30.9%, 0%, 32.1%, 23.1%, 7.7%, 60.3% and 38.5% respectively.ConclusionThe proportion of isolates resistant to CIP, TMP-SMZ, CRO, AC, E and V were low compared to AP, PG and Ox. S. aureus is normally resident in humans; therefore, the S. aureus present in the cow’s milk may have resulted from transmission between the two species, emphasizing the need to improve sanitary conditions in the milking environment.

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Section: Introductionmentioning

confidence: 99%

Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia

Daka

Solomon

silassie

et al. 2012

Ann Clin Microbiol Antimicrob

115

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“…And the use of various primers for P. aeruginosa generated a total of 131 amplified fragments of between 200 to 300 bp, this more than the number of fragments generated from Staphylococcus aureus and Escherichia coli while using the same number of primers (Onasanya et al, 2003;Salehi et al, 2008;Shehata, 2008).…”

Section: Results and Dicussionmentioning

confidence: 99%

Antimicrobial activity of commonly used antibiotics and DNA fingerprint analysis of Pseudomonas aeruginosa obtained from clinical isolates and unchlorinated drinking water in Korea, 2010

Kim

Lee

et al. 2011

Arch. Pharm. Res.

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Pseudomonas aeruginosa exists in various environments, and can cause mild or serious infections resulting in a wide range of symptoms. In this study, we collected bacterial isolates from hospitalized patients and unchlorinated drinking water, in Korea, 2010. The water-borne and clinical isolates were compared using colony morphology, antimicrobial susceptibility testing, and random amplification of polymorphic DNA analysis. We first compared morphological features of the water-borne and clinical isolates. The clearest difference in colony morphology was colony shape; five water-borne isolate colonies (83%) had a smooth, circular morphology, while nine (75%) clinical isolate colonies had a rough, irregular morphology. Minimum inhibitory concentrations analyses were performed to determine antimicrobial resistant patterns; using ceftazidime, gentamicin, tigecycline, chloramphenicol, meropenem, and tobramycin according to Clinical and Laboratory Standard Institute (CLSI, 2009) methodology. All waterborne isolates were not resistant to gentamicin, tobramycin, and meropenem. The clinical isolates were resistant to every antibiotic except chloramphenicol. Genotyping was performed using the repetitive extragenic palindromic polymerase-chain-reaction. The DNA fingerprinting patterns did not reveal genetic similarity between the water-borne and clinical P. aeruginosa isolates. On the contrary, they showed that genetically distinct populations have been established in each of these environments. We have revealed significant morphological, clinical and genetic differences between water-borne and clinical isolates of the same bacterial species.

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“…Isolates were able to be grouped into 23 genotypes at 50% similarity coefficient (Figure 7). Onasanya et al (2003) reported two major groups of Staphylococcus aureus at 50% similarity coefficient, while 12 different subgroups were obtained at 100% similarity coefficient. Genotype 7 was more prevalent (20%) followed by genotype 8 and 9 (10%) (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

In Silico Virulence and Resistance Profile Analysis of Staphylococcus species

Nahar¹,

Rashid²,

Kabir³

et al. 2017

Bangla Pharma J

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In silico studies of the genes of Staphylococcus spp. might establish some correlations with multiple pathological factors. Sixty isolates of Staphylococcus spp. have been studied here targeting virulence and antibiotic resistance genes through in silico tools. Here, in silico PCR (polymerase chain reaction) amplification detected both virulence and antibiotic resistance genes. Study revealed that most of the isolates harboured either cap5 (40%) or cap8 (31.67%) locus gene. Staphylococcal enterotoxin was detected in 63.33% of the isolates. The sea gene, responsible for food poisoning, was detected in 26.67% of the isolates. The tst positive isolates (5%), responsible for toxic shock syndrome, were present in only genotype 8. No exfoliative toxin was detected. The icaA gene, responsible for intracellular adherence, appeared in 80% of the isolates. Alpha hemolysin gene, hla, was detected in 63.33% of the isolates. Sixty-five percent of the isolates harboured the mecA genes. Both β-lactamase (blaZ) and erythromycin resistance, ermA genes were available in 38.33% of the isolates. In silico pulsed field gel electrophoresis (PFGE) digestion was able to divide isolates into 23 genotypes. Genotype 8 and 11 harboured tetracycline resistance genes, tetM and tetK. The tetM gene (18.33%) was more prevalent than tetK gene (11.67%). Genotype 1 and 11 were considered more virulent than others. Genotype 11 also carried six antibiotic resistance genes but did not carry the genes msrA, msrB, ermB and ermC. The data generated here might aid in the prediction of the virulence and resistance profile based on genotyping as well as contribute in vaccine development.

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Genetic fingerprinting and phylogenetic diversity of Staphylococcus aureus isolates from Nigeria

Cited by 27 publications

References 18 publications

Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia

Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia

Antimicrobial activity of commonly used antibiotics and DNA fingerprint analysis of Pseudomonas aeruginosa obtained from clinical isolates and unchlorinated drinking water in Korea, 2010

In Silico Virulence and Resistance Profile Analysis of Staphylococcus species

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