Antimicrobial activity of commonly used antibiotics and DNA fingerprint analysis of Pseudomonas aeruginosa obtained from clinical isolates and unchlorinated drinking water in Korea, 2010

Abstract

Pseudomonas aeruginosa exists in various environments, and can cause mild or serious infections resulting in a wide range of symptoms. In this study, we collected bacterial isolates from hospitalized patients and unchlorinated drinking water, in Korea, 2010. The water-borne and clinical isolates were compared using colony morphology, antimicrobial susceptibility testing, and random amplification of polymorphic DNA analysis. We first compared morphological features of the water-borne and clinical isolates. The clearest difference in colony morphology was colony shape; five water-borne isolate colonies (83%) had a smooth, circular morphology, while nine (75%) clinical isolate colonies had a rough, irregular morphology. Minimum inhibitory concentrations analyses were performed to determine antimicrobial resistant patterns; using ceftazidime, gentamicin, tigecycline, chloramphenicol, meropenem, and tobramycin according to Clinical and Laboratory Standard Institute (CLSI, 2009) methodology. All waterborne isolates were not resistant to gentamicin, tobramycin, and meropenem. The clinical isolates were resistant to every antibiotic except chloramphenicol. Genotyping was performed using the repetitive extragenic palindromic polymerase-chain-reaction. The DNA fingerprinting patterns did not reveal genetic similarity between the water-borne and clinical P. aeruginosa isolates. On the contrary, they showed that genetically distinct populations have been established in each of these environments. We have revealed significant morphological, clinical and genetic differences between water-borne and clinical isolates of the same bacterial species.

Genotypic Investigation of Multidrug-Resistant Pseudomonas aeruginosa from Clinical Isolates in Korea, 2010

“…Among the VIM types, VIM-2 is the most dominant worldwide and was particularly detected in Europe (Livermore and Woodford, 2000). Furthermore, carbapenem-resistant MBL-possessing P. aeruginosa has been reported with increasing frequency in Korea (Oh et al, 2003;Lee et al, 2009;Kim et al, 2011). In this study, we analyzed the levels of multidrug-resistant clinical P. aeruginosa isolated from a secondary hospital in Korea in 2010.…”

Isolation and Identification of Pseudomonas aeruginosa in Natural Environments by International Organization for Standardization ISO/NP 16266

“…Pseudomonas aeruginosa는 자연적 방어가 손상된 사람에게 감염되는 기회주의적 병원체로, 병원, 물, 토양 및 대기 등 많은 자연과 인공환경에서 서식한다 (Lee et al, 1999;Arora et al, 2011;Bae, 2005;Kim et al, 2011;Mahmoud et al, 2013). P. aeruginosa 는 병원 내 감염 (Kang et al, 2011)과 다중 내성 (Hota et al, 2009 (Table 1).…”