Genetic Diversity of the Critically Endangered  Thuja sutchuenensis Revealed by ISSR Markers and  the Implications for Conservation

Liu, Jianfeng; Shi, S G; Chang, Ermei; Wen-juan, Yang; Jiang, Zeping

doi:10.3390/ijms140714860

Cited by 46 publications

(31 citation statements)

References 37 publications

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“…Patterns of high genetic diversity at both the species' and local population levels have already been documented in other mountain plants [35] and critically endangered species [36], [37], especially when relatively large populations have been considered [38]. Our findings, however, were in contrast with two studies on F. imperialis and F. camschatcensis using RAPD markers, where variation within populations accounted for only 54% and 35.7% of the total variation, respectively [32], [39].…”

Section: Discussioncontrasting

confidence: 92%

Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

2014

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Fritillaria tubiformis subsp. moggridgei Rix. is a rare alpine geophyte with shiny yellow flowers. This plant is sporadically distributed across the southwestern Alps where it is biogeographically close to F. tubiformis var. burnatii Planch. The latter has dark purple flowers and ranges in the majority of the Western and Central Alps. In order to develop appropriate strategies of conservation, a RAPD based analysis was conducted to study the genetic status of these taxa and the distribution of genetic variability of the subspecies by sampling seven populations distributed across the subspecies' range. Four populations of var. burnatii were chosen within this range and included in the genetic analysis. Some 264 individuals were analysed and 201 polymorphic loci were scored. Genetic diversity scored in the subspecies was in line with expectations for endemic species (He = 0.194). F. tubiformis var. burnatii showed lower intraspecific diversity (He = 0.173), notwithstanding a wider range than the subspecies. Most of the total phenotypic variation (about 83%) was allocated within populations, and significant lower proportions between taxa (6.45%) and between populations of the same taxon (10.64%). Moreover, PCoA analysis and Bayesian clustering separated populations into two genetically differentiated groups corresponding with the subspecific taxa. However, three populations ascribed to the subsp. moggridgei repeatedly showed genetic admixture with var. burnatii populations. Our findings suggest that: i) although the different flower colour, the two taxa are genetically very similar and share a consistent part of their gene pool, ii) the majority of genetic variability is allocated within populations rather than among them, iii) a representative amount of genetic diversity can be preserved by sampling from a restricted number of populations. The efficacy of RAPD markers in analysing genetic variation, and the contribution of the results to the preservation of biodiversity of the species, are discussed.

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Section: Discussioncontrasting

confidence: 92%

Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

2014

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“…(80.0%) (Santana et al, 2011), Thuja sutchuenensis Franch. (76.1%) (Liu et al, 2013), Larix gmelinii (Rupr.) (98.8%) (Zhang et al, 2013) and Erythrina velutina Willd (98.0%) (Gonçalves et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.

Araújo

Pacheco

Vieira

et al. 2016

Idesia

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Knowledge of the genetic diversity of the threatened tree species in the Brazilian semiarid region may contribute to the creation of effective strategies for their preservation and future use. The aim of this study was to select ISSR (Inter-Simple Sequence Repeat) primers capable of detecting genetic polymorphism among Mimosa caesalpiniaefolia Benth. individuals for a study of the genetic diversity of this species. A total of 27 ISSR primers were tested for their ability to amplify genomic DNA of three individuals (bulk); all of them amplified regions between the simple sequence repeats of the genome, however seven ISSR primers showed a good standard of amplification compared to the others. The seven primers selected were used to amplify the genomic DNA of nine individuals and generated a total of 78 loci, of which 52.7% were polymorphic. The content of polymorphic information of the primers ranged from 0.261 to 0.489, showing that selected primers are moderately informative; bootstrap analysis determined that 51 loci were enough to estimate the genetic diversity of the samples of individuals. We concluded that the markers generated by the primers UBC 807, UBC 824, UBC 827, UBC 840, UBC 851, UBC 873 and UBC 881 allow detection of the genetic polymorphism among individuals of M. caesalpiniaefolia, being useful to determine the genetic diversity of this species.Key words: semiarid, forest species, primers, polymorphism. RESUMEN Conocer la diversidad genética de las especies de árboles forestales en la región semiárida de Brasil puede contribuir al desarrollo de estrategias eficaces para su conservación y uso futuro. El objetivo de este estudio fue seleccionar iniciadores de ISSR (Secuencias

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“…Therefore, systematic management of depleting bamboo resource through the adoption of proper conservation strategies with either in situ or ex situ measures is the need of hour in the region. Assessing the level of genetic variation within and among natural bamboo populations is highly crucial for the development of effective conservation methods [7–10]. This is because the ability of a particular plant species to adapt effectively to changing environmental conditions depends on the level of genetic variability it possesses [11, 12].…”

Section: Introductionmentioning

confidence: 99%

ISSR Marker Based Population Genetic Study ofMelocanna baccifera(Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

et al. 2017

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Melocanna baccifera (Roxb.) Kurz is an economically important bamboo of North-East India experiencing population depletion in its natural habitats. Genetic variation studies were conducted in 7 populations sampled from 5 districts of Manipur using ISSR molecular markers. The investigation was carried out as a primary step towards developing effective conservation strategies for the protection of bamboo germplasm. ISSR marker analysis showed significant level of genetic variation within the populations as revealed by moderately high average values of Nei's genetic diversity (H 0.1639), Shannon's diversity index (I 0.2563), percentage of polymorphic bands (PPB 59.18), total genetic variation (Ht 0.1961), and genetic diversity within population (Hs 0.1639). The study also divulged a high genetic variation at species level with Shannon's diversity index (I), Nei's genetic diversity (H), and percentage of polymorphic band (PPB%) recorded at 0.3218, 0.1939, and 88.37, respectively. Genetic differentiation among the populations (Gst) was merely 19.42% leaving 80.58% of genetic variation exhibited within the populations. The low genetic diversity between populations was consistent with AMOVA. The low genetic differentiation among populations coupled with existence of significantly high genetic diversity at species level indicated the urgent necessity of preserving and protecting all the existing natural bamboo populations in the region.

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Genetic Diversity of the Critically Endangered Thuja sutchuenensis Revealed by ISSR Markers and the Implications for Conservation

Cited by 46 publications

References 37 publications

Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.

ISSR Marker Based Population Genetic Study ofMelocanna baccifera(Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India

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