Thuja sutchuenensis Franch. is a critically endangered plant endemic to the North-East Chongqing, China. Genetic variation was studied to assess the distribution of genetic diversity within and among seven populations from the single remnant locations, using inter-simple sequence repeat (ISSR) markers. A total of 15 primers generated 310 well defined bands, with an average of 20.7 bands per primer. The seven populations revealed a relatively high level of genetic diversity in the species. The percentage of polymorphic bands, Nei’s gene diversity and Shannon’s information index at the population and species level were 76.1%, 0.155, 0.252 and 100%, 0.165, 0.295, respectively. A low level of genetic differentiation among populations (GST = 0.102), in line with the results of Analyses of Molecular Variance (AMOVA), and a high level of gene flow (Nm = 4.407) were observed. Both the Unweighted Pair Group Method with Arithmatic Mean (UPGMA) cluster analysis and Principal Coordinates Analysis (PCoA) supported the grouping of all seven populations into two groups. In addition, Mantel test revealed no significant correlation between genetic and geographical distances (r = 0.329, p = 0.100). The low genetic differentiation among populations implies that the conservation efforts should aim to preserve all the extant populations of this endangered species.
ABSTRACT. The population of Magnolia sprengeri individuals deceased drastically in the late 20th century because of the widespread harvest for traditional Chinese medicinal recipes. In this study, the levels of phenotypic variation and the genetic structure of 2 populations of M. sprengeri were estimated. The phenotypic variation of M. sprengeri characteristics was nonsynchronous, with a coefficient of variation for 37 characters from 9.55-35.87%. The variance stabilizing transformation value ranged from 0.034-52.344%. The variation contribution within the population was greater than the contribution among the population; the among-population rate was 2.864%, while the within-population rate was 15.849%; values of repeatability for among-population and within-population were 0.430 and 0.098, respectively. This indicates that more variation arose from withinpopulation and that population repeatability was much greater than individual repeatability. Variation in the flower organ was greater than that in the leaf organ; this means that vegetative variation was more stable than reproductive variation. Variation in the southern population was greater than that in the northern population.
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