Genetic diversity of symbiotic bacteria nodulating common bean (Phaseolus vulgaris) in western Kenya

Kawaka, Fanuel; Makonde, Huxley Mae; Dida, Mathews M.; Opala, P. A.; Ombori, Omwoyo; Maingi, John M.; John, McCormick

doi:10.1371/journal.pone.0207403

Cited by 19 publications

(13 citation statements)

References 42 publications

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“…Further, the good performance of control, could be associated with native strain adaptation to the ecological conditions of the study area. These results are in agreement with the findings by Kawaka et al [23] who reported SEF ranging between 67 and 170% when common beans were inoculated with native rhizobia. High performance of commercial R probably, could be associated with the commercial strains being more adapted to the study area compared to the native strains, leading to higher performance in SEF.…”

Section: Conclusion and Recommendationssupporting

confidence: 93%

Integrated Effect of Rhizobium Inoculation and Phosphorus Application on Tissue Content, Symbiotic and Phosphorus use Efficiency in Soybean Production

Mulambula¹,

Gathungu²,

Ndukhu³

et al. 2019

IJEAB

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Soybean (Glycine max) is an important legume crop that is cultivated all over the world as livestock feed, food for human consumption, soil fertility improvement and industrial products such as candles and paints. However, Nitrogen (N) and phosphorus (P) nutrient have been attributed to the decline in soybean yields. Furthermore, scanty information is documented on P-efficient soybean genotypes, which are a sustainable P management strategy for enhancing symbiotic efficiency (SEF) and phosphorus use efficiency (PUE). As a solution, field experiment was conducted at Chuka University farm to evaluate the integration effect of rhizobium inoculation (R) and P on tissue nutrient content, SEF and PUE in soybean production in Meru South Sub County. Two cultivations (Trial I and II) were done in 2018. Treatments included; three rates of R (0, 100 and 200 g ha -1 ), three rates of P (0, 20 and 30 kgha -1 ), either applied alone or integrated and soybean genotypes (SB19 and SB24). Both Trials were laid out in a randomized complete block design in split-split plot arrangement with each treatment replicated thrice. Genotypes were assigned main plot, R subplots and P in sub-subplots. Data collected was subjected to analysis of variance using the Scientific Analysis System SAS and significantly different means separated using Tukey test at (p≤0.05). The results showed significant difference in N and P tissue content, SEF and PUE for SB19 and SB24 genotypes in both Trials at (p≤0.05). The highest N tissue content of between 1.73% and 9.10% was observed when integration of R and P were applied at the rate of 200 g and 30 kg for SB19 and SB24 in both Trials. While R and P at the rate of 200 g and 30 kg per ha showed the highest P content of between 849.6 ppm and 955.0 ppm in both Trials. The highest SEF recorded was 207% and 261%, and 201% and 227% in Trials I and II, respectively. The PUE was highest when R and P was applied at the rate of 200 g and 30 kg per ha for SB19 and SB24 soybean in both Trials. Integration of R and P at the rate of 200 g and 30 kg ha -1 and adoption of either SB19 or SB24 showed a potential in enhancing soybean cultivation.

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Section: Conclusion and Recommendationssupporting

confidence: 93%

Integrated Effect of Rhizobium Inoculation and Phosphorus Application on Tissue Content, Symbiotic and Phosphorus use Efficiency in Soybean Production

Mulambula¹,

Gathungu²,

Ndukhu³

et al. 2019

IJEAB

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“…The number of effective nodules was estimated by observing the internal colour of the nodule ( Kawaka et al., 2018 ; Thrall et al., 2011 ). The nodules were cut into two halves to observe the colour of the interior.…”

Section: Methodsmentioning

confidence: 99%

Application of biochar and inorganic phosphorus fertilizer influenced rhizosphere soil characteristics, nodule formation and phytoconstituents of cowpea grown on tropical soil

Phares

Atiah

Frimpong

et al. 2020

Heliyon

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The effect of biochar alone or co-applied with triple superphosphate on rhizosphere soil characteristics, nodule formation, phytoconstituents and antioxidant property of cowpea ( Vigna uguiculata ) is yet to be adequately examined in sub Saharan Africa. A field experiment was conducted where cowpea ( Vigna unguiculata ) was grown in a tropical sandy loam soil amended with biochar at 1.5 t ha −1 and 2.5 t ha −1 solely or together with inorganic phosphate fertilizer (Triple superphosphate), applied at a rate of 60 kg P ha −1 . At 50% flowering, changes in selected rhizosphere soil properties (pH, total nitrogen, available phosphorus, soil organic carbon, cation exchange capacity), nodule count, phytochemicals (phenols, flavonoids, alkaloids, tannins, saponins) and antioxidant property of cowpea roots and leaves were determined by standard laboratory procedures. Differences between means of the measured parameters were established using ANOVA, and relationships among the parameters were explored using Pearson correlation (p < 0.05). Addition of biochar solely or in combination with TSP significantly (p < 0.05) increased soil pH, total nitrogen, available phosphorus, soil organic carbon, cation exchange capacity and root nodule count. Flavonoids, phenols, alkaloids, saponin, tannin contents and antioxidant activity in the roots and leaves were significantly (p < 0.05) higher in the amended soils compared with the unamended soil. Similarly, soil flavonoids, phenols, alkaloids and antioxidant activity were significantly higher in amended soils compared with control. Significant, positive inter and intra correlation with varying strength was found between soil properties, nodule number and phytoconstituents. This is an indication that biochar can be co-applied with triple superphosphate to sustain soil fertility, improve nodulation and enhance concentrations of phytoconstituents in soil, cowpea roots and leaves.

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“…However, higher nodulation performance by Rhizobium etli strains containing a nodC type α than the strains that contain a nodC type δ in Mesoamerican beans is considered a good example of prior selection of strains for nodulation in bean plants (19, 40, 41). More than 27 species of rhizobia have been isolated from bean (30, 42–46), among which Rhizobium tropici (47), R. etli (48), R. phaseoli (49), R.…”

Section: Introductionmentioning

confidence: 99%

Genetic Interaction Studies Reveal Superior Performance of Rhizobium tropici CIAT899 on a Range of Diverse East African Common Bean (Phaseolus vulgaris L.) Genotypes

Gunnabo

Geurts

Wolde-meskel³

et al. 2019

Appl Environ Microbiol

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The existence of genotype-by-strain (GL × GR) interaction has implications for the expected stability of performance of legume inoculants and could represent both challenges and opportunities for improvement of nitrogen fixation. We find that significant genotype-by-strain interaction exists in common bean (Phaseolus vulgaris L.) but that the strength and direction of this interaction depends on the growing environment used to evaluate biomass. Strong genotype and strain main effects, combined with a lack of predictable patterns in GL × GR, suggests that at best individual bean genotypes and strains can be selected for superior additive performance. The observation that the screening environment may affect experimental outcome of GL × GR means that identified patterns should be corroborated under more realistic conditions.

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Genetic diversity of symbiotic bacteria nodulating common bean (Phaseolus vulgaris) in western Kenya

Cited by 19 publications

References 42 publications

Integrated Effect of Rhizobium Inoculation and Phosphorus Application on Tissue Content, Symbiotic and Phosphorus use Efficiency in Soybean Production

Integrated Effect of Rhizobium Inoculation and Phosphorus Application on Tissue Content, Symbiotic and Phosphorus use Efficiency in Soybean Production

Application of biochar and inorganic phosphorus fertilizer influenced rhizosphere soil characteristics, nodule formation and phytoconstituents of cowpea grown on tropical soil

Genetic Interaction Studies Reveal Superior Performance of Rhizobium tropici CIAT899 on a Range of Diverse East African Common Bean (Phaseolus vulgaris L.) Genotypes

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