Background There is paucity of information on antibiotics utilization amongst farmers, factors associated with administration of antibiotics and farmers' knowledge of the effects of antibiotics on the soil ecosystem in Ghana. Methods A cross sectional quantitative survey across three coastal regions of Ghana was undertaken amongst poultry and livestock farmers. Six hundred respondents were selected from five districts each across the three regions. Pretested and structured questionnaire were used to collect data through face to face interview. Data were summarized using descriptive statistics and regression analysis. Factors associated with antibiotic administration were determined using binary multiple logistic regression at p � 0.05. Results Out of the 600 farmers, 95% administered antibiotics and 84% bought antibiotics over-thecounter without prescription. Approximately 9% of antibiotic administration was carried out by veterinary officers, and the remaining, 91% based on farmer's experience. Approximately 93% had access to antibiotics without any difficulty. Withdrawal period was always observed by only 16% of farmers. Majority (74%) of farmers never had education on antibiotics and none of the farmers screened manure for the antibiotic residuals. Years of farming, income status, level of education of farmers, type of animal kept, access to extension services, registration with farmers' association, employing veterinary services, location of farm, system of production, education on antibiotics and access to antibiotics positively and significantly predicted the administration of antibiotics by farmers. Majority of farmers had inadequate knowledge of the effects of antibiotics on soil ecosystem with mean score ranging between 2.87±0.60 and 2.98 ± 0.7 on a scale of 5.0.

The effect of biochar alone or co-applied with triple superphosphate on rhizosphere soil characteristics, nodule formation, phytoconstituents and antioxidant property of cowpea ( Vigna uguiculata ) is yet to be adequately examined in sub Saharan Africa. A field experiment was conducted where cowpea ( Vigna unguiculata ) was grown in a tropical sandy loam soil amended with biochar at 1.5 t ha −1 and 2.5 t ha −1 solely or together with inorganic phosphate fertilizer (Triple superphosphate), applied at a rate of 60 kg P ha −1 . At 50% flowering, changes in selected rhizosphere soil properties (pH, total nitrogen, available phosphorus, soil organic carbon, cation exchange capacity), nodule count, phytochemicals (phenols, flavonoids, alkaloids, tannins, saponins) and antioxidant property of cowpea roots and leaves were determined by standard laboratory procedures. Differences between means of the measured parameters were established using ANOVA, and relationships among the parameters were explored using Pearson correlation (p < 0.05). Addition of biochar solely or in combination with TSP significantly (p < 0.05) increased soil pH, total nitrogen, available phosphorus, soil organic carbon, cation exchange capacity and root nodule count. Flavonoids, phenols, alkaloids, saponin, tannin contents and antioxidant activity in the roots and leaves were significantly (p < 0.05) higher in the amended soils compared with the unamended soil. Similarly, soil flavonoids, phenols, alkaloids and antioxidant activity were significantly higher in amended soils compared with control. Significant, positive inter and intra correlation with varying strength was found between soil properties, nodule number and phytoconstituents. This is an indication that biochar can be co-applied with triple superphosphate to sustain soil fertility, improve nodulation and enhance concentrations of phytoconstituents in soil, cowpea roots and leaves.

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The preparation and application of biochar by smallholder farmers is labour intensive hence an effective one-time application for multiple cropping seasons would be desirable by farmers and researchers. In this study, one-time biochar application as a soil amendment and its interaction with compost and NPK on yield performances of different crops was investigated across three cropping seasons. Treatments included biochar applied alone or together with compost, inorganic NPK fertilizer or both. Maize, okra and cassava were planted in succession and data was collected on their shoot N, P and K concentrations, yields as well as selected soil parameters (pH, exchangeable acidity, total exchangeable bases, effective cation exchange capacity, total N, total organic carbon, available phosphorus). Data was analyzed with GenSTAT and results were presented in tables and bar graph. Corn cob biochar applied solely did not significantly improve maize and okra yield in the first and second cropping season but increased yield of cassava significantly at the third cropping season. Yield increased in sole NPK, compost and NPK + compost treatments for all cropping cycles, but yields obtained from these treatments in the presence of biochar were greater than their corresponding treatments without biochar. The study also showed that biochar application together with compost, NPK or both, improved total organic carbon, total nitrogen, available phosphorus, total exchangeable bases, exchangeable acidity, effective cation exchange capacity and pH as well as tissue N, P and K of all crops. Our findings demonstrated that a single application of biochar, particularly in the presence of compost, inorganic NPK fertilizer or both can increase yields across three cropping seasons and improve soil fertility.

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Introduction: The use of biochar to restore soil fertility is still in the exploratory stages in Ghana and there is paucity of information regarding the effect of biochar on soil biochemical properties. An experiment was conducted to evaluate the effect of biochar solely applied or in combination with poultry manure on the composition of soil phosphorus solubilizing fungi, available P concentration and selected properties of Oxisol in Ghana. Methods: Cocoa husk biochar (CHB), prepared using Lucia biomass pyrolytic stove at a temperature of 400°C was applied solely at 0, 39 and 65 t ha-1 and in combination with 10 t ha-1 , poultry manure, to the soil. Treatments were arranged in a completely randomized design with three replications. Results: The population of phosphorus solubilizing fungi increased in amended soils significantly Original Research Article

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