Genetic diversity of sea-island cotton (Gossypium barbadense) revealed by mapped SSRs

Abstract

ABSTRACT. In order to evaluate the genetic diversity of seaisland cotton (Gossypium barbadense), 237 commonly mapped SSR markers covering the cotton genome were used to genotype 56 seaisland cotton accessions. A total of 218 polymorphic primer pairs (91.98%) amplified 361 loci, with a mean of 1.66 loci. Polymorphism information content values of the SSR primers ranged from 0.035 to 0.862, with a mean of 0.320. The highest mean polymorphism information content value for the SSR motifs was from a compound motif (0.402), and for the chromosomes it was Chr10 (0.589); the highest ratio of polymorphic primers in Xinjiang accessions was from Chr21 (83.33%). Genetic diversity was high in Xinjiang accessions. AMOVA showed that variation was 8 and 92% among populations and within populations, respectively. The 56 sea-island accessions were divided into three groups in the UPGMA dendrogram: Xinhai5 was in the first group; accessions from Xinjiang, except the five main ones, were in the second group, and the other 34 accessions ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4): 3620-3631 (2011) Genetic diversity of sea-island cotton by mapped SSRs 3621were in the third group. Accessions from the former Soviet Union and Xinjiang main accessions were closely related. Both PCA and UPGMA confirmed that Xinhai5 was distinct from the other accessions, and accessions from Xinjiang were in an independent group. Given the differences between principal components analysis and UPGMA results, it is necessary to combine molecular markers and pedigree information so that genetic diversity can be objectively analyzed.

Comparison of wild accessions of g. barbadense l. from Peru and Brazil via microsatellite markers

“…Dissimilarity mean, in relation to the total accessions, was higher for Pima S7, followed by PI528329; and lower for PI528243, followed by MG04-43. This range of distance index is also close to those obtained with SSR within G. barbadense L. (WANG et al, 2011;ABDELLATIF et al, 2012).…”

“…Wu et al (2010) evaluated accessions from the former USSR and from Egypt, China and the USA, and observed 1.62 alleles per locus SSR, and similarity index between 0.66 to 0.94, and mean of 0.81. Wang et al (2011) also evaluated cultivars from China, and introductions from other countries, with SSR, and reported PIC between 0.03 and 0.86, and mean of 0.32. Finally, Abdellatif et al (2012) evaluate varieties and hybrids from Egypt with ISSR, EST and SSR, and reported PIC values between 0.8 and 0.91, and mean of 0.86 for SSR.…”

Assessing genetic diversity among six populations of Gossypium arboreum L. using microsatellites markers

“…Present analysis showed significant difference among population groups, among individuals of a group and within individuals (p = 0.001). In previous similar study by Wang et al (2011), 92 % of total variation was found confined to within population variation whereas only 8 % of total variation was due to among population variation, as analyzed by AMOVA. Noormohammadi et al (2013), in diploid cotton genotypes after analysis by AMOVA, found 2 % of total variation due to population groups and 98 % due to genetic variations among accessions in each population group; such a low polymorphism among population groups could be due to inclusion of inter-specific hybrids, second backcross progenies and F 5 plants of the same cross.…”

Genetic dissection of upland cotton (Gossypium hirsutum) cultivars developed in Hubei Province by mapped SSRs

“…Polymerase chain reaction (PCR), electrophoresis and silver staining were performed as previously described by Wang et al (2011).…”

“…DNA molecular markers were the most effective method of genetic analysis at the DNA level. In cotton, various molecular markers have been applied in genetic diversity analysis such as simple sequence repeats (SSRs), rapid amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism, inter-SSR, and sequence-related amplified polymorphism; the cotton species included Gossypium hirsutum (Xu et al, 2001;Rana et al, 2005;Zhang et al, 2005;Wu et al, 2010;Kalivas et al, 2011), G. barbadense (Westengen et al, 2005;El-Zanaty et al, 2011;Wang et al, 2011), and G. arboretum (Liu et al, 2006;Kantartzi et al, 2009). …”